We are currently updating the database - data may be missing for the next 10 minutes. We apologize for any inconvenience.

Human Metabolome Database Version 2.5

 

Showing metabocard for 25-Hydroxycholesterol (HMDB06247)

Legend: metabolite field enzyme field

Version 2.5
Creation Date 2007-05-22 20:16:51
Update Date 2009-05-05 21:00:45
Accession Number HMDB06247
Secondary Accession Numbers Not Available
Common Name 25-Hydroxycholesterol
Description 25-Hydroxycholesterol is steroid derivative that suppresses the cleavage of sterol regulatory element binding proteins (SREBPs). It also induces apoptosis through down-regulation of Bcl-2 expression and activation of caspases. 25-Hydroxycholesterol also enhances Interleukin-1 beta (IL-1beta-induced) IL-8 production.(PMID: 17086498 ). 25-hydroxycholesterol is endogenously produced from cholesterol at early time intervals after cholesterol ingestion. It inhibits HMG-CoA reductase and so it also plays a significant role in the in vivo regulation of cholesterol biosynthesis after an acute dietary cholesterol challenge.
Synonyms
  1. (beta)-cholest-5-ene-3-25-diol
  2. 25-hydroxycholest-5-en-3-ol
  3. 25-hydroxy-cholesterol
  4. 5-cholestene-3beta-25-diol
  5. cholest-5-ene-3beta-25-diol
  6. cholest-5-en-3beta-25-diol
  7. Cholest-5-ene-3-beta,25-diol
  8. Cholest-5-ene-3-b,25-diol
Chemical IUPAC Name (3S,8S,9S,10R,13R,14S,17R)-17-[(2R)-6-hydroxy-6-methyl-heptan-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol
Chemical Formula C27H46O2
Chemical Structure Structure
Chemical Taxonomy
Kingdom
  • Organic
Super Class
  • Cholesterols and derivatives
Class
  • Steroids and Steroid Derivatives
Sub Class
  • Dihydroxy steroids
Family
  • Mammalian Metabolite
Species
  • secondary alcohol
  • tertiary alcohol
  • alkene
Biofunction
  • Hormones, Membrane component
Application
—
Source
  • Endogenous
Average Molecular Weight 402.653
Monoisotopic Molecular Weight 402.349792
Isomeric SMILES C[C@H](CCCC(C)(C)O)[C@H]1CCC2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)C3CC[C@]12C
Canonical SMILES CC(CCCC(C)(C)O)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C
KEGG Compound ID C15519 Link Image
BioCyc ID CPD-7241 Link Image
BiGG ID 2197441 Link Image
Wikipedia Link Not Available
NuGOwiki Link HMDB06247 Link Image
Metagene Link HMDB06247 Link Image
METLIN ID 3899 Link Image
PubChem Compound 65094 Link Image
PubChem Substance 14781420 Link Image
ChEBI ID 37616 Link Image
CAS Registry Number 2140-46-7
InChI Identifier InChI=1/C27H46O2/c1-18(7-6-14-25(2,3)29)22-10-11-23-21-9-8-19-17-20(28)12-15-26(19,4)24(21)13-16-27(22,23)5/h8,18,20-24,28-29H,6-7,9-17H2,1-5H3/t18-,20+,21+,22-,23+,24?,26+,27-/m1/s1
Synthesis Reference Ryer, August I.; Gebert, Wm. H. Steroids. (1954), US 2673206 19540323 CAN 49:8565 AN 1955:8565
Melting Point (Experimental) 172-173 oC
Experimental Water Solubility Not Available Source: PhysProp
Predicted Water Solubility 2.44e-04 mg/mL [Predicted by ALOGPS] Calculated using ALOGPS
Physiological Charge 0
State Solid
Experimental LogP/Hydrophobicity Not Available Source: PhysProp
Predicted LogP/Hydrophobicity 5.95 [Predicted by ALOGPS]; 6.7 [Predicted by PubChem via XLOGP] Calculated using ALOGPS
Material Safety Data Sheet (MSDS) Not Available
MOL File Show
SDF File Show
PDB File Show
2D Structure
3D Structure
Experimental PDB ID Not Available
Experimental 1H NMR Spectrum Not Available
Experimental 13C NMR Spectrum Not Available
Experimental 13C HSQC Spectrum Not Available
Predicted 1H NMR Spectrum Show Image
Show Peaklist
Predicted 13C NMR Spectrum Show Image
Show Peaklist
Mass Spectrum Not Available
Simplified TOCSY Spectrum Not Available
BMRB Spectrum Not Available
Cellular Location
  • endoplasmic reticulum
Biofluid Location Not Available
Tissue Location Not Available
Concentrations (Normal) Not Available
Concentrations (Abnormal) Not Available
Associated Disorders Not Available
OMIM ID Not Available
Pathways
Name SMPDB Link KEGG Link
Bile Acid Biosynthesis SMP00035 Link Image map00120 Link Image
General References Not Available
Metabolic Enzymes
  1. Cytochrome P450 7B1
  2. Oxysterol-binding protein 1
  3. Cholesterol 25-hydroxylase
  4. Cholesterol 25-hydroxylase
  5. Cytochrome P450, family 7, subfamily B, polypeptide 1
Enzyme 1 [top]
Enzyme 1 ID 6952
Enzyme 1 Name Cytochrome P450 7B1
Enzyme 1 Synonyms
  1. 25-hydroxycholesterol 7-alpha- hydroxylase
  2. Oxysterol 7-alpha-hydroxylase
Enzyme 1 Gene Name CYP7B1
Enzyme 1 Protein Sequence >Cytochrome P450 7B1 
MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVL
NLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLE
KAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAEL
YPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKS
IREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPT
MFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEAL
RLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEEFRYDRFIE
DGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILLTYFDLEIIDDKPI
GLNYSRLLFGIQYPDSDVLFRYKVKS
Enzyme 1 Number of Residues 506
Enzyme 1 Molecular Weight 58257
Enzyme 1 Theoretical pI 8.13
Enzyme 1 GO Classification
Function
  • binding
  • catalytic activity
  • cation binding
  • heme binding
  • ion binding
  • iron ion binding
  • monooxygenase activity
  • oxidoreductase activity
  • tetrapyrrole binding
  • transition metal ion binding
Process
  • cellular metabolism
  • electron transport
  • generation of precursor metabolites and energy
  • metabolism
  • physiological process
Component
—
Enzyme 1 General Function Secondary metabolites biosynthesis, transport and catabolism
Enzyme 1 Specific Function Cholest-5-ene-3-beta,25-diol + NADPH + O(2) = cholest-5-ene-3-beta,7-alpha,25-triol + NADP(+) + H(2)O
Enzyme 1 Pathways Not Available
Enzyme 1 Reactions Not Available
Enzyme 1 Pfam Domain Function
Enzyme 1 Signals
  • 1-40
Enzyme 1 Transmembrane Regions
  • 287-309
Enzyme 1 Essentiality Not Available
Enzyme 1 GenBank ID Protein 3702794 Link Image
Enzyme 1 UniProtKB/Swiss-Prot ID O75881 Link Image
Enzyme 1 UniProtKB/Swiss-Prot Entry Name CP7B1_HUMAN Link Image
Enzyme 1 PDB ID Not Available
Enzyme 1 Cellular Location Not Available
Enzyme 1 Gene Sequence >1521 bp 
ATGGCAGGAGAAGTGTCCGCGGCCACGGGCCGCTTTTCGCTGGAGCGGTTGGGCCTCCCG
GGCCTGGCCCTCGCCGCGGCCCTGCTGCTCCTGGCCCTCTGCTTGCTTGTCCGGCGCACC
AGGAGACCCGGTGAGCCTCCATTGATAAAAGGTTGGCTTCCTTATCTTGGAGTGGTCCTG
AACTTACGAAAAGACCCCTTAAGGTTCATGAAAACACTTCAAAAGCAACATGGTGACACT
TTCACAGTTCTTCTTGGTGGAAAGTACATAACATTTATCCTGGACCCCTTCCAGTACCAG
CTAGTGATAAAAAATCATAAACAATTAAGCTTTCGAGTATTTTCTAATAAATTATTAGAG
AAAGCATTTAGCATCAGTCAGTTGCAAAAAAATCATGACATGAATGATGAGCTTCACCTC
TGCTATCAATTTTTGCAAGGCAAATCTTTGGACATACTCTTGGAAAGCATGATGCAGAAT
CTAAAACAAGTTTTTGAACCCCAGCTGTTAAAAACCACAAGTTGGGACACGGCAGAACTG
TATCCATTCTGCAGCTCAATAATATTTGAGATCACATTTACAACTATATATGGAAAAGTT
ATTGTTTGTGACAACAACAAATTTATTAGTGAGCTAAGAGATGATTTTTTAAAATTTGAT
GACAAGTTTGCATATTTAGTATCCAACATACCCATTGAGCTTCTAGGAAATGTCAAGTCT
ATTAGAGAGAAAATTATAAAATGCTTCTCATCAGAAAAGTTAGCCAAGATGCAAGGATGG
TCAGAAGTTTTTCAAAGCAGGCAAGATGTCCTGGAGAAATATTATGTGCACGAGGACCTT
GAAATAGGAGCACATCATTTAGGCTTTCTCTGGGCCTCTGTGGCAAACACTATTCCAACT
ATGTTCTGGGCAATGTATTATCTTCTGCGGCACCCAGAAGCTATGGCAGCAGTGCGTGAC
GAAATTGACCATTTGCTGCAGTCAACAGGTCAAAAGAAAGGGTCTGGATTTCCCATCCAC
CTCACCAGAGAACAATTGGACAGCCTAATCTGCCTAGAAAGCAGCATTTTTGAAGCTTTA
CGACTGTCCTCATATTCAACCACCATTCGTTTTGTTGAGGAGGATTTGACTCTCAGTTCA
GAGACCGGGGACTACTGTGTGCGAAAGGGAGACTTGGTAGCCATCTTTCCTCCAGTCCTA
CATGGTGACCCTGAAATCTTTGAAGCTCCAGAGGAGTTTAGATATGATCGTTTTATAGAA
GATGGTAAGAAGAAAACCACCTTTTTCAAAAGAGGGAAAAAGCTGAAGTGTTACCTAATG
CCGTTTGGAACTGGAACCAGCAAATGTCCAGGCCGATTTTTTGCACTTATGGAAATAAAA
CAATTGTTGGTTATACTTTTAACTTATTTTGATTTAGAAATAATTGATGATAAGCCCATA
GGACTAAACTACAGCCGCTTGTTGTTTGGTATTCAGTATCCAGATTCTGATGTTTTATTT
AGATACAAAGTGAAATCTTAG
Enzyme 1 GenBank Gene ID AF029403 Link Image
Enzyme 1 GeneCard ID CYP7B1 Link Image
Enzyme 1 GenAtlas ID CYP7B1 Link Image
Enzyme 1 HGNC ID HGNC:2652 Link Image
Enzyme 1 Chromosome Location 8
Enzyme 1 Locus 8q21.3
Enzyme 1 SNPs SNPJam Report Link Image
Enzyme 1 General References
  1. Setchell KD, Schwarz M, O'Connell NC, Lund EG, Davis DL, Lathe R, Thompson HR, Weslie Tyson R, Sokol RJ, Russell DW: Identification of a new inborn error in bile acid synthesis: mutation of the oxysterol 7alpha-hydroxylase gene causes severe neonatal liver disease. J Clin Invest. 1998 Nov 1;102(9):1690-703. [PubMed Link Image]
  2. Wu Z, Martin KO, Javitt NB, Chiang JY: Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1. J Lipid Res. 1999 Dec;40(12):2195-203. [PubMed Link Image]
Enzyme 1 Metabolite References Not Available
Enzyme 2 [top]
Enzyme 2 ID 8336
Enzyme 2 Name Oxysterol-binding protein 1
Enzyme 2 Synonyms Not Available
Enzyme 2 Gene Name OSBP
Enzyme 2 Protein Sequence >Oxysterol-binding protein 1 
MAATELRGVVGPGPAAIAALGGGGAGPPVVGGGGGRGDAGPGSGAASGTVVAAAAGGPGP
GAGGVAAAGPAPAPPTGGSGGSGAGGSGSAREGWLFKWTNYIKGYQRRWFVLSNGLLSYY
RSKAEMRHTCRGTINLATANITVEDSCNFIISNGGAQTYHLKASSEVERQRWVTALELAK
AKAVKMLAESDESGDEESVSQTDKTELQNTLRTLSSKVEDLSTCNDLIAKHGTALQRSLS
ELESLKLPAESNEKIKQVNERATLFRITSNAMINACRDFLMLAQTHSKKWQKSLQYERDQ
RIRLEETLEQLAKQHNHLERAFRGATVLPANTPGNVGSGKDQCCSGKGDMSDEDDENEFF
DAPEIITMPENLGHKRTGSNISGASSDISLDEQYKHQLEETKKEKRTRIPYKPNYSLNLW
SIMKNCIGKELSKIPMPVNFNEPLSMLQRLTEDLEYHELLDRAAKCENSLEQLCYVAAFT
VSSYSTTVFRTSKPFNPLLGETFELDRLEENGYRSLCEQVSHHPPAAAHHAESKNGWTLR
QEIKITSKFRGKYLSIMPLGTIHCIFHATGHHYTWKKVTTTVHNIIVGKLWIDQSGEIDI
VNHKTGDKCNLKFVPYSYFSRDVARKVTGEVTDPSGKVHFALLGTWDEKMECFKVQPVIG
ENGGDARQRGHEAEESRVMLWKRNPLPKNAENMYYFSELALTLNAWESGTAPTDSRLRPD
QRLMENGRWDEANAEKQRLEEKQRLSRKKREAEAMKATEDGTPYDPYKALWFERKKDPVT
KELTHIYRGEYWECKEKQDWSSCPDIF
Enzyme 2 Number of Residues 807
Enzyme 2 Molecular Weight 89421
Enzyme 2 Theoretical pI 7.31
Enzyme 2 GO Classification
Function
—
Process
  • cellular lipid metabolism
  • lipid metabolism
  • metabolism
  • physiological process
  • primary metabolism
  • steroid metabolism
Component
—
Enzyme 2 General Function Not Available
Enzyme 2 Specific Function May play a role in the regulation of sterol metabolism. Binds a range of oxysterols
Enzyme 2 Pathways Not Available
Enzyme 2 Reactions Not Available
Enzyme 2 Pfam Domain Function
Enzyme 2 Signals
  • None
Enzyme 2 Transmembrane Regions
  • None
Enzyme 2 Essentiality Not Available
Enzyme 2 GenBank ID Protein 189403 Link Image
Enzyme 2 UniProtKB/Swiss-Prot ID P22059 Link Image
Enzyme 2 UniProtKB/Swiss-Prot Entry Name OSBP1_HUMAN Link Image
Enzyme 2 PDB ID Not Available
Enzyme 2 Cellular Location Not Available
Enzyme 2 Gene Sequence >2424 bp 
ATGGCGGCGACGGAGCTGAGAGGAGTGGTGGGGCCAGGCCCGGCAGCCATTGCAGCACTT
GGCGGCGGCGGCGCCGGTCCCCCAGTGGTGGGAGGAGGCGGCGGCCGCGGAGATGCGGGG
CCAGGCTCCGGGGCCGCGTCAGGGACGGTGGTCGCGGCGGCGGCGGGAGGCCCGGGCCCG
GGGGCCGGGGGAGTGGCGGCGGCTGGCCCGGCCCCTGCGCCGCCGACTGGGGGCTCGGGC
GGCTCGGGCGCTGGGGGTTCGGGCTCGGCTCGAGAGGGCTGGCTCTTCAAATGGACCAAT
TATATCAAAGGCTACCAGCGGCGATGGTTCGTGCTGAGCAACGGGCTCCTGAGCTACTAC
AGATCAAAGGCAGAAATGAGACATACCTGCCGTGGTACCATCAACCTCGCCACAGCCAAC
ATCACCGTGGAGGACTCCTGCAACTTCATCATTTCCAATGGGGGTGCTCAGACCTACCAT
CTGAAAGCTAGTTCAGAAGTTGAGCGGCAGCGCTGGGTGACGGCCCTGGAACTGGCCAAG
GCCAAAGCTGTGAAGATGCTGGCAGAGTCAGATGAATCAGGAGATGAAGAGTCTGTCTCA
CAAACTGACAAGACTGAGCTGCAGAATACCCTTCGGACCCTCTCTAGCAAAGTAGAGGAC
TTGAGCACGTGCAATGACTTGATAGCTAAGCATGGCACAGCTCTGCAGCGTTCTCTCAGT
GAGCTGGAGTCCCTGAAGTTGCCTGCTGAGAGCAATGAAAAGATCAAACAGGTCAACGAA
CGAGCCACACTCTTTAGGATAACATCCAATGCCATGATCAACGCCTGCAGAGATTTCCTC
ATGTTAGCCCAGACCCATAGTAAAAAATGGCAAAAGTCACTACAGTATGAAAGAGACCAG
CGTATCCGACTGGAAGAAACCCTCGAGCAGCTGGCGAAGCAGCATAATCACCTGGAGAGG
GCCTTCCGAGGAGCCACGGTGCTGCCGGCAAACACTCCTGGCAATGTGGGTTCTGGTAAA
GATCAGTGCTGCTCTGGCAAAGGGGACATGAGCGATGAAGATGATGAGAATGAATTTTTT
GATGCACCTGAGATCATCACCATGCCTGAAAATTTGGGCCACAAACGTACTGGCAGCAAT
ATCAGTGGAGCCAGCAGTGACATCAGCCTTGATGAACAGTACAAGCATCAGCTGGAGGAG
ACCAAAAAGGAAAAGAGAACCAGAATACCATACAAGCCAAACTATAGCCTCAATTTATGG
AGCATCATGAAGAACTGCATTGGAAAAGAACTCTCTAAGATCCCCATGCCGGTAAACTTT
AATGAGCCCTTGTCCATGCTTCAGCGCCTTACTGAAGATCTGGAATACCATGAGCTGTTA
GACCGAGCTGCAAAATGTGAGAATTCTCTAGAACAGCTCTGTTATGTTGCAGCTTTCACC
GTGTCCTCCTACTCCACTACTGTCTTCCGCACCAGTAAGCCATTCAACCCACTGCTTGGG
GAGACCTTTGAGCTGGACCGATTAGAGGAGAATGGGTACCGATCCCTCTGTGAACAGGTG
AGTCATCATCCCCCTGCTGCTGCGCACCATGCTGAGTCCAAAAATGGCTGGACATTGCGT
CAGGAAATCAAAATCACCAGCAAGTTTCGAGGCAAATACCTCTCCATTATGCCCCTCGGT
ACCATTCATTGTATTTTCCATGCAACTGGGCACCACTACACTTGGAAGAAAGTTACCACA
ACTGTACACAACATTATTGTGGGCAAGTTGTGGATAGATCAGTCTGGCGAAATTGATATT
GTGAATCACAAGACAGGAGACAAGTGTAATCTTAAATTTGTTCCTTATAGCTACTTCTCT
CGGGATGTAGCAAGAAAGGTGACGGGGGAAGTGACAGATCCATCAGGAAAAGTCCACTTT
GCTCTTCTGGGGACGTGGGATGAGAAAATGGAATGTTTCAAAGTACAGCCAGTCATTGGG
GAAAATGGGGGTGATGCTCGACAGAGAGGCCATGAAGCAGAGGAAAGCAGGGTCATGCTG
TGGAAAAGGAATCCTTTACCGAAGAATGCAGAAAACATGTACTACTTCTCAGAGCTTGCT
CTGACTCTCAATGCTTGGGAAAGTGGCACTGCCCCCACAGACAGCCGGTTACGACCTGAC
CAGAGACTGATGGAAAATGGACGCTGGGATGAAGCAAATGCGGAGAAGCAGCGCCTGGAG
GAAAAACAAAGACTTTCCAGAAAGAAGAGAGAAGCGGAAGCTATGAAAGCCACAGAGGAT
GGCACACCATATGATCCCTATAAGGCACTGTGGTTTGAGCGGAAGAAGGACCCTGTTACC
AAGGAGTTAACCCATATTTATAGGGGAGAATACTGGGAGTGTAAAGAAAAACAGGACTGG
AGCTCATGCCCGGACATTTTCTGA
Enzyme 2 GenBank Gene ID M86917 Link Image
Enzyme 2 GeneCard ID OSBP Link Image
Enzyme 2 GenAtlas ID OSBP Link Image
Enzyme 2 HGNC ID HGNC:8503 Link Image
Enzyme 2 Chromosome Location 11
Enzyme 2 Locus 11q12-q13
Enzyme 2 SNPs SNPJam Report Link Image
Enzyme 2 General References
  1. Levanon D, Hsieh CL, Francke U, Dawson PA, Ridgway ND, Brown MS, Goldstein JL: cDNA cloning of human oxysterol-binding protein and localization of the gene to human chromosome 11 and mouse chromosome 19. Genomics. 1990 May;7(1):65-74. [PubMed Link Image]
  2. Moreira EF, Jaworski C, Li A, Rodriguez IR: Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina. J Biol Chem. 2001 May 25;276(21):18570-8. Epub 2001 Jan 26. [PubMed Link Image]
Enzyme 2 Metabolite References Not Available
Enzyme 3 [top]
Enzyme 3 ID 8578
Enzyme 3 Name Cholesterol 25-hydroxylase
Enzyme 3 Synonyms
  1. Cholesterol 25- monooxygenase
  2. h25OH
Enzyme 3 Gene Name CH25H
Enzyme 3 Protein Sequence >Cholesterol 25-hydroxylase 
MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDI
LCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPE
LLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELF
SLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHD
LHHSHFNCNFAPYFTHWDKILGTLRTASVPAR
Enzyme 3 Number of Residues 272
Enzyme 3 Molecular Weight 31745
Enzyme 3 Theoretical pI 7.26
Enzyme 3 GO Classification
Function
  • catalytic activity
Process
  • metabolism
  • physiological process
Component
—
Enzyme 3 General Function Lipid transport and metabolism
Enzyme 3 Specific Function Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25- hydroxycholesterol by macrophages may play a role in Leydig cell differentiation
Enzyme 3 Pathways Not Available
Enzyme 3 Reactions Not Available
Enzyme 3 Pfam Domain Function
Enzyme 3 Signals Not Available
Enzyme 3 Transmembrane Regions
  • 38-58 84-104 121-141
Enzyme 3 Essentiality Not Available
Enzyme 3 GenBank ID Protein 4038304 Link Image
Enzyme 3 UniProtKB/Swiss-Prot ID O95992 Link Image
Enzyme 3 UniProtKB/Swiss-Prot Entry Name CH25H_HUMAN Link Image
Enzyme 3 PDB ID Not Available
Enzyme 3 Cellular Location Not Available
Enzyme 3 Gene Sequence >819 bp 
ATGAGCTGCCACAACTGCTCCGACCCCCAGGTCCTTTGCAGCTCCGGGCAGCTGTTCCTG
CAGCCCCTCTGGGACCACCTGAGGAGCTGGGAGGCCCTCCTACAGTCGCCCTTCTTCCCG
GTCATCTTCTCCATCACCACATACGTGGGCTTTTGCCTGCCCTTCGTGGTCCTGGATATC
CTGTGCTCCTGGGTGCCCGCCCTGCGGCGCTACAAGATCCACCCTGACTTCTCGCCATCC
GCGCAGCAGCTGCTACCTTGCCTGGGGCAGACCCTCTACCAGCATGTGATGTTTGTGTTC
CCCGTGACGCTGCTGCATTGGGCCCGCAGCCCGGCCCTCCTGCCCCACGAAGCTCCCGAG
CTGCTCCTGCTGCTGCACCACATCCTGTTCTGCCTGCTACTCTTCGACATGGAGTTCTTC
GTGTGGCACCTGCTGCACCACAAGGTGCCCTGGCTGTACCGCACCTTCCACAAGGTGCAC
CACCAGAACTCGTCCTCGTTCGCGCTGGCAACGCAGTATATGAGCGTCTGGGAACTGTTT
TCTTTGGGCTTCTTCGACATGATGAACGTCACACTGCTCGGGTGCCACCCGCTCACCACC
CTGACCTTCCACGTGGTCAACATCTGGCTTTCCGTGGAGGACCACTCCGGCTACAACTTC
CCTTGGTCCACTCACAGACTGGTGCCCTTCGGGTGGTACGGGGGTGTGGTGCACCACGAC
CTGCATCACTCTCACTTTAACTGCAACTTCGCTCCGTACTTTACACACTGGGACAAAATA
CTGGGAACGCTGCGGACTGCATCTGTCCCAGCGCGGTGA
Enzyme 3 GenBank Gene ID AF059212 Link Image
Enzyme 3 GeneCard ID CH25H Link Image
Enzyme 3 GenAtlas ID CH25H Link Image
Enzyme 3 HGNC ID HGNC:1907 Link Image
Enzyme 3 Chromosome Location 10
Enzyme 3 Locus 10q23
Enzyme 3 SNPs SNPJam Report Link Image
Enzyme 3 General References
  1. Lund EG, Kerr TA, Sakai J, Li WP, Russell DW: cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism. J Biol Chem. 1998 Dec 18;273(51):34316-27. [PubMed Link Image]
Enzyme 3 Metabolite References Not Available
Enzyme 4 [top]
Enzyme 4 ID 16474
Enzyme 4 Name Cholesterol 25-hydroxylase
Enzyme 4 Synonyms
  1. SubName: cDNA, FLJ95765, Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA
Enzyme 4 Gene Name CH25H
Enzyme 4 Protein Sequence >Cholesterol 25-hydroxylase 
MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDI
LCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPE
LLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELF
SLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHD
LHHSHFNCNFAPYFTHWDKILGTLRTASVPAR
Enzyme 4 Number of Residues 272
Enzyme 4 Molecular Weight 31745
Enzyme 4 Theoretical pI 7.26
Enzyme 4 GO Classification Not Available
Enzyme 4 General Function Lipid transport and metabolism
Enzyme 4 Specific Function Not Available
Enzyme 4 Pathways Not Available
Enzyme 4 Reactions Not Available
Enzyme 4 Pfam Domain Function
Enzyme 4 Signals
  • None
Enzyme 4 Transmembrane Regions
  • None
Enzyme 4 Essentiality Not Available
Enzyme 4 GenBank ID Protein Not Available
Enzyme 4 UniProtKB/Swiss-Prot ID B2RBY3 Link Image
Enzyme 4 UniProtKB/Swiss-Prot Entry Name B2RBY3_HUMAN Link Image
Enzyme 4 PDB ID Not Available
Enzyme 4 Cellular Location Not Available
Enzyme 4 Gene Sequence Not Available
Enzyme 4 GenBank Gene ID AK314865 Link Image
Enzyme 4 GeneCard ID B2RBY3 Link Image
Enzyme 4 GenAtlas ID Not Available
Enzyme 4 HGNC ID Not Available
Enzyme 4 Chromosome Location 10
Enzyme 4 Locus 10q23
Enzyme 4 SNPs SNPJam Report Link Image
Enzyme 4 General References Not Available
Enzyme 4 Metabolite References Not Available
Enzyme 5 [top]
Enzyme 5 ID 16524
Enzyme 5 Name Cytochrome P450, family 7, subfamily B, polypeptide 1
Enzyme 5 Synonyms Not Available
Enzyme 5 Gene Name CYP7B1
Enzyme 5 Protein Sequence >Cytochrome P450, family 7, subfamily B, polypeptide 1 
MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVL
NLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLE
KAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAEL
YPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKS
IREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPT
MFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEAL
RLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEEFRYDRFIE
DGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILLTYFDLEIIDDKPI
GLNYSRLLFGIQYPDSDVLFRYKVKS
Enzyme 5 Number of Residues 506
Enzyme 5 Molecular Weight 58257
Enzyme 5 Theoretical pI 8.13
Enzyme 5 GO Classification
Function
  • binding
  • catalytic activity
  • cation binding
  • heme binding
  • ion binding
  • iron ion binding
  • monooxygenase activity
  • oxidoreductase activity
  • tetrapyrrole binding
  • transition metal ion binding
Process
  • cellular metabolism
  • electron transport
  • generation of precursor metabolites and energy
  • metabolism
  • physiological process
Component
—
Enzyme 5 General Function Secondary metabolites biosynthesis, transport and catabolism
Enzyme 5 Specific Function Not Available
Enzyme 5 Pathways Not Available
Enzyme 5 Reactions Not Available
Enzyme 5 Pfam Domain Function
Enzyme 5 Signals
  • None
Enzyme 5 Transmembrane Regions
  • None
Enzyme 5 Essentiality Not Available
Enzyme 5 GenBank ID Protein Not Available
Enzyme 5 UniProtKB/Swiss-Prot ID B2RN07 Link Image
Enzyme 5 UniProtKB/Swiss-Prot Entry Name B2RN07_HUMAN Link Image
Enzyme 5 PDB ID Not Available
Enzyme 5 Cellular Location Not Available
Enzyme 5 Gene Sequence Not Available
Enzyme 5 GenBank Gene ID BC136574 Link Image
Enzyme 5 GeneCard ID B2RN07 Link Image
Enzyme 5 GenAtlas ID Not Available
Enzyme 5 HGNC ID Not Available
Enzyme 5 Chromosome Location Not Available
Enzyme 5 Locus Not Available
Enzyme 5 SNPs SNPJam Report Link Image
Enzyme 5 General References Not Available
Enzyme 5 Metabolite References Not Available