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Record Information
Version5.0
StatusDetected and Quantified
Creation Date2005-11-16 15:48:42 UTC
Update Date2021-09-14 15:45:29 UTC
HMDB IDHMDB0001126
Secondary Accession Numbers
  • HMDB01126
Metabolite Identification
Common NameNornicotine
DescriptionNornicotine, also known as (+-)-nornicotine or DL-norcitine, belongs to the class of organic compounds known as pyrrolidinylpyridines. Pyrrolidinylpyridines are compounds containing a pyrrolidinylpyridine ring system, which consists of a pyrrolidine ring linked to a pyridine ring. In humans, nornicotine is involved in the nicotine metabolism pathway. Nornicotine has been detected, but not quantified in, a few different foods, such as anatidaes (Anatidae), chickens (Gallus gallus), and domestic pigs (Sus scrofa domestica). This could make nornicotine a potential biomarker for the consumption of these foods. Nornicotine is a primary metabolite. Primary metabolites are metabolically or physiologically essential metabolites. They are directly involved in an organism’s growth, development or reproduction. Based on a literature review a small amount of articles have been published on Nornicotine.
Structure
Data?1582752178
Synonyms
ValueSource
(+-)-1'-Demethyl-nicotineHMDB
(+-)-1'-DemethylnicotineHMDB
(+-)-3-(2-Pyrrolidinyl)pyridineHMDB
(+-)-NornicotineHMDB
(R)-3-(Pyrrolidin-2-yl)pyridineHMDB
(R,S)-NornicotineHMDB
(S)-2-(3-Pyridyl)pyrrolidineHMDB
2-(3-Pyridyl)pyrrolidineHMDB
3-(2-Pyrrolidinyl)pyridineHMDB
DL-NorcitineHMDB
L-Nor-nicotineHMDB
NornicotinHMDB
S-(-)-NornicotineHMDB
Nornicotine tartrate, (S)-(R-(r*,r*))-isomerHMDB
Nornicotine, (R)-isomerHMDB
Nornicotine, (S)-isomerHMDB
Chemical FormulaC9H12N2
Average Molecular Weight148.205
Monoisotopic Molecular Weight148.100048394
IUPAC Name3-(pyrrolidin-2-yl)pyridine
Traditional Name(+-)-nornicotine
CAS Registry Number494-97-3
SMILES
C1CNC(C1)C1=CC=CN=C1
InChI Identifier
InChI=1S/C9H12N2/c1-3-8(7-10-5-1)9-4-2-6-11-9/h1,3,5,7,9,11H,2,4,6H2
InChI KeyMYKUKUCHPMASKF-UHFFFAOYSA-N
Chemical Taxonomy
Description Belongs to the class of organic compounds known as pyrrolidinylpyridines. Pyrrolidinylpyridines are compounds containing a pyrrolidinylpyridine ring system, which consists of a pyrrolidine ring linked to a pyridine ring.
KingdomOrganic compounds
Super ClassOrganoheterocyclic compounds
ClassPyridines and derivatives
Sub ClassPyrrolidinylpyridines
Direct ParentPyrrolidinylpyridines
Alternative Parents
Substituents
  • Pyrrolidinylpyridine
  • Aralkylamine
  • Heteroaromatic compound
  • Pyrrolidine
  • Azacycle
  • Secondary amine
  • Secondary aliphatic amine
  • Organic nitrogen compound
  • Organopnictogen compound
  • Hydrocarbon derivative
  • Organonitrogen compound
  • Amine
  • Aromatic heteromonocyclic compound
Molecular FrameworkAromatic heteromonocyclic compounds
External Descriptors
Ontology
Disposition

Biological location

Source

Route of exposure

Process

Naturally occurring process

Physical Properties
StateLiquid
Experimental Molecular Properties
PropertyValueReference
Melting PointNot AvailableNot Available
Boiling Point270 °CNot Available
Water SolubilityNot AvailableNot Available
LogP0.17HANSCH,C ET AL. (1995)
Experimental Chromatographic PropertiesNot Available
Predicted Molecular Properties
PropertyValueSource
Water Solubility26 g/LALOGPS
logP10(0.66) g/LALOGPS
logP10(0.78) g/LChemAxon
logS10(-0.76) g/LALOGPS
pKa (Strongest Basic)10.21ChemAxon
Physiological Charge1ChemAxon
Hydrogen Acceptor Count2ChemAxon
Hydrogen Donor Count1ChemAxon
Polar Surface Area24.92 ŲChemAxon
Rotatable Bond Count1ChemAxon
Refractivity44.36 m³·mol⁻¹ChemAxon
Polarizability16.66 ųChemAxon
Number of Rings2ChemAxon
BioavailabilityYesChemAxon
Rule of FiveYesChemAxon
Ghose FilterNoChemAxon
Veber's RuleYesChemAxon
MDDR-like RuleNoChemAxon
Predicted Chromatographic Properties

Predicted Collision Cross Sections

PredictorAdduct TypeCCS Value (Å2)Reference
DarkChem[M+H]+132.05831661259
DarkChem[M-H]-129.55931661259
AllCCS[M+H]+131.87632859911
AllCCS[M-H]-134.50532859911
DeepCCS[M+H]+134.28730932474
DeepCCS[M-H]-130.86130932474
DeepCCS[M-2H]-168.12730932474
DeepCCS[M+Na]+143.66630932474

Predicted Kovats Retention Indices

Underivatized

MetaboliteSMILESKovats RI ValueColumn TypeReference
NornicotineC1CNC(C1)C1=CC=CN=C11963.0Standard polar33892256
NornicotineC1CNC(C1)C1=CC=CN=C11361.6Standard non polar33892256
NornicotineC1CNC(C1)C1=CC=CN=C11391.7Semi standard non polar33892256

Derivatized

Derivative Name / StructureSMILESKovats RI ValueColumn TypeReference
Nornicotine,1TMS,isomer #1C[Si](C)(C)N1CCCC1C1=CC=CN=C11549.8Semi standard non polar33892256
Nornicotine,1TMS,isomer #1C[Si](C)(C)N1CCCC1C1=CC=CN=C11515.2Standard non polar33892256
Nornicotine,1TMS,isomer #1C[Si](C)(C)N1CCCC1C1=CC=CN=C11924.5Standard polar33892256
Nornicotine,1TBDMS,isomer #1CC(C)(C)[Si](C)(C)N1CCCC1C1=CC=CN=C11777.0Semi standard non polar33892256
Nornicotine,1TBDMS,isomer #1CC(C)(C)[Si](C)(C)N1CCCC1C1=CC=CN=C11780.7Standard non polar33892256
Nornicotine,1TBDMS,isomer #1CC(C)(C)[Si](C)(C)N1CCCC1C1=CC=CN=C12134.6Standard polar33892256
Spectra

GC-MS Spectra

Spectrum TypeDescriptionSplash KeyDeposition DateSourceView
Experimental GC-MSGC-MS Spectrum - Nornicotine GC-EI-TOF (Non-derivatized)splash10-0006-1910000000-7aaa8177fe27a116f3e42017-09-12HMDB team, MONA, MassBankView Spectrum
Experimental GC-MSGC-MS Spectrum - Nornicotine GC-EI-TOF (Non-derivatized)splash10-0006-1910000000-7aaa8177fe27a116f3e42018-05-18HMDB team, MONA, MassBankView Spectrum
Predicted GC-MSPredicted GC-MS Spectrum - Nornicotine GC-MS (Non-derivatized) - 70eV, Positivesplash10-00xr-4900000000-b1dd2fa89d2e3a6004b72017-09-01Wishart LabView Spectrum
Predicted GC-MSPredicted GC-MS Spectrum - Nornicotine GC-MS (Non-derivatized) - 70eV, PositiveNot Available2021-10-12Wishart LabView Spectrum
Predicted GC-MSPredicted GC-MS Spectrum - Nornicotine GC-MS (Non-derivatized) - 70eV, PositiveNot Available2021-10-12Wishart LabView Spectrum

MS/MS Spectra

Spectrum TypeDescriptionSplash KeyDeposition DateSourceView
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QTOF , positive-QTOFsplash10-0002-0900000000-f2b0fa8b9c12821f2a962017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QTOF , positive-QTOFsplash10-001i-0900000000-f9d65ea20e70d97b17a72017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QTOF , positive-QTOFsplash10-00lr-0900000000-c15db03324adc5374c112017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QTOF , positive-QTOFsplash10-0159-0900000000-c74af7c19e9329720e4e2017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QTOF , positive-QTOFsplash10-0159-0900000000-128439754c8d0a69bec02017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-001j-0900000000-fbf9379b4dd1ba5915d12017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-001j-0900000000-3268f0d1161f7c767e392017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-001i-1900000000-641ce2610f3b89a028532017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-001i-2900000000-b3d63962e4ee5a7567ff2017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-001i-3900000000-73abee1b55e5f70341cb2017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-00lr-5900000000-df09cb3a6316364590042017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-00lr-9800000000-a45b0ff544bcd0281db02017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-0159-9300000000-13f626ee37faf55341532017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine LC-ESI-QFT , positive-QTOFsplash10-0fsc-9100000000-53e714e4328ef96344a92017-09-14HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine 40V, Positive-QTOFsplash10-0159-0900000000-c74af7c19e9329720e4e2021-09-20HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine 30V, Positive-QTOFsplash10-00lr-0900000000-c03060e07c24d91fe2b72021-09-20HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine 10V, Positive-QTOFsplash10-0002-0900000000-6ffb5e4450055085bf5f2021-09-20HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine 10V, Positive-QTOFsplash10-0002-0900000000-d4059a90a539229953c92021-09-20HMDB team, MONAView Spectrum
Experimental LC-MS/MSLC-MS/MS Spectrum - Nornicotine 20V, Positive-QTOFsplash10-001i-0900000000-dabe42a76f889cfb99382021-09-20HMDB team, MONAView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - Nornicotine 10V, Positive-QTOFsplash10-0002-0900000000-59737c2da8553c7ab03f2016-06-03Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - Nornicotine 20V, Positive-QTOFsplash10-0002-1900000000-c7de13e17b1e82a46e3f2016-06-03Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - Nornicotine 40V, Positive-QTOFsplash10-0v5c-9200000000-8eeb64be10e289cb93032016-06-03Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - Nornicotine 10V, Negative-QTOFsplash10-0002-0900000000-1f7637ef9abf28475e712016-08-03Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - Nornicotine 20V, Negative-QTOFsplash10-0002-3900000000-4580e77240a12f45be982016-08-03Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - Nornicotine 40V, Negative-QTOFsplash10-017l-9500000000-1af339f4c1a6a2b7ae6a2016-08-03Wishart LabView Spectrum

NMR Spectra

Spectrum TypeDescriptionDeposition DateSourceView
Predicted 1D NMR13C NMR Spectrum (1D, 100 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 100 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 1000 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 1000 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 200 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 200 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 300 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 300 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 400 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 400 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 500 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 500 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 600 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 600 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 700 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 700 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 800 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 800 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR13C NMR Spectrum (1D, 900 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Predicted 1D NMR1H NMR Spectrum (1D, 900 MHz, D2O, predicted)2021-09-29Wishart LabView Spectrum
Biological Properties
Cellular Locations
  • Cytoplasm
Biospecimen Locations
  • Blood
  • Urine
Tissue Locations
  • Kidney
  • Liver
Pathways
Normal Concentrations
BiospecimenStatusValueAgeSexConditionReferenceDetails
BloodExpected but not QuantifiedNot QuantifiedNot AvailableNot AvailableNormal
      Not Available
details
Abnormal Concentrations
BiospecimenStatusValueAgeSexConditionReferenceDetails
UrineDetected and Quantified0.20 (0.01-0.40) umol/mmol creatinineAdult (>18 years old)BothActive tobacco user details
Associated Disorders and Diseases
Disease References
Smoking
  1. Moyer TP, Charlson JR, Enger RJ, Dale LC, Ebbert JO, Schroeder DR, Hurt RD: Simultaneous analysis of nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine by tandem mass spectrometry, with clinically relevant metabolic profiles. Clin Chem. 2002 Sep;48(9):1460-71. [PubMed:12194923 ]
Associated OMIM IDsNone
DrugBank IDNot Available
Phenol Explorer Compound IDNot Available
FooDB IDFDB022439
KNApSAcK IDC00042792
Chemspider ID21108497
KEGG Compound IDC06524
BioCyc IDNot Available
BiGG IDNot Available
Wikipedia LinkNornicotine
METLIN ID1534
PubChem Compound412
PDB IDNot Available
ChEBI ID28313
Food Biomarker OntologyNot Available
VMH IDNot Available
MarkerDB IDNot Available
Good Scents IDNot Available
References
Synthesis ReferencePapadopoulos, Nick M. Formation of nornicotine and other metabolites from nicotine in vitro and in vivo. Canadian Journal of Biochemistry and Physiology (1964), 42(4), 435-42.
Material Safety Data Sheet (MSDS)Download (PDF)
General References
  1. Moyer TP, Charlson JR, Enger RJ, Dale LC, Ebbert JO, Schroeder DR, Hurt RD: Simultaneous analysis of nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine by tandem mass spectrometry, with clinically relevant metabolic profiles. Clin Chem. 2002 Sep;48(9):1460-71. [PubMed:12194923 ]
  2. Idris AM, Prokopczyk B, Hoffmann D: Toombak: a major risk factor for cancer of the oral cavity in Sudan. Prev Med. 1994 Nov;23(6):832-9. [PubMed:7855117 ]
  3. Schweinsberg F, Sander J, Schweinsberg E, Kollat P: [Examinations of the possibilities to nitrosate nicotine and nornicotine and of the formation of N-nitrosonornicotine in the stomach of smokers (author's transl)]. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1975 Sep 22;84(1):81-7. [PubMed:127438 ]
  4. Jacob P 3rd, Yu L, Liang G, Shulgin AT, Benowitz NL: Gas chromatographic-mass spectrometric method for determination of anabasine, anatabine and other tobacco alkaloids in urine of smokers and smokeless tobacco users. J Chromatogr. 1993 Sep 8;619(1):49-61. [PubMed:8245163 ]
  5. Xu X, Iba MM, Weisel CP: Simultaneous and sensitive measurement of anabasine, nicotine, and nicotine metabolites in human urine by liquid chromatography-tandem mass spectrometry. Clin Chem. 2004 Dec;50(12):2323-30. Epub 2004 Oct 7. [PubMed:15472033 ]