| Identification |
|---|
| HMDB Protein ID
| HMDBP00349 |
| Secondary Accession Numbers
| |
| Name
| Heparan sulfate glucosamine 3-O-sulfotransferase 4 |
| Synonyms
|
- 3-OST-4
- Heparan sulfate 3-O-sulfotransferase 4
- Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4
- h3-OST-4
|
| Gene Name
| HS3ST4 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in sulfotransferase activity |
| Specific Function
| Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity).
|
| Pathways
|
Not Available
|
| Reactions
|
| Phosphoadenosine phosphosulfate + [heparan sulfate]-glucosamine → Adenosine 3',5'-diphosphate + [heparan sulfate]-glucosamine 3-sulfate |
details
|
|
| GO Classification
|
| Biological Process |
| heparan sulfate proteoglycan metabolic process |
| Cellular Component |
| extracellular region |
| integral to membrane |
| Golgi membrane |
| Function |
| catalytic activity |
| transferase activity |
| transferase activity, transferring sulfur-containing groups |
| sulfotransferase activity |
| Molecular Function |
| [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity |
|
| Cellular Location
|
- Golgi apparatus membrane
- Single-pass type II membrane protein (Probable)
|
| Gene Properties |
|---|
| Chromosome Location
| 16 |
| Locus
| 16p11.2 |
| SNPs
| HS3ST4 |
| Gene Sequence
|
>1371 bp
ATGGCCCGGTGGCCCGCACCTCCTCCGCCTCCGCCTCCGCCTCCACCTCTGGCCGCGCCG
CCGCCGCCCGGCGCCTCTGCTAAGGGGCCGCCGGCGCGCAAGCTGCTTTTTATGTGCACC
TTGTCCCTGTCTGTCACCTACCTGTGCTACAGCCTCCTGGGCGGCTCGGGCTCCCTGCAA
TTCCCTCTGGCGCTGCAGGAGTCGCCGGGCGCCGCCGCCGAGCCCCCGCCGAGCCCGCCG
CCACCCTCTCTGCTGCCTACCCCCGTGCGCCTCGGCGCCCCCTCGCAGCCGCCCGCGCCG
CCGCCGCTGGACAACGCGAGCCACGGGGAGCCGCCCGAGCCCCCAGAGCAGCCAGCCGCC
CCCGGGACCGACGGCTGGGGGCTGCCGAGCGGCGGCGGAGGCGCCCGGGACGCCTGGCTC
CGGACCCCGCTGGCCCCCAGCGAGATGATCACGGCTCAGAGCGCGCTGCCGGAGAGGGAA
GCGCAGGAGTCCAGCACCACCGACGAGGATCTCGCAGGCCGGAGAGCGGCCAACGGGAGC
AGCGAGAGGGGCGGCGCCGTCAGCACCCCCGACTATGGGGAGAAGAAGCTGCCACAGGCG
CTCATCATCGGGGTCAAGAAAGGAGGGACCCGCGCGCTGCTGGAGGCGATCCGCGTGCAC
CCGGACGTGCGGGCGGTGGGCGTAGAGCCGCACTTCTTCGACAGGAACTACGAAAAGGGG
TTGGAGTGGTACAGAAATGTGATGCCCAAGACTTTGGATGGGCAAATAACCATGGAGAAG
ACTCCAAGTTACTTTGTGACAAATGAGGCTCCCAAGCGCATTCACTCCATGGCCAAGGAC
ATCAAACTGATTGTGGTGGTGAGAAACCCCGTGACCAGGGCCATCTCTGACTACACGCAG
ACACTGTCAAAGAAACCCGAGATCCCCACCTTTGAGGTGCTGGCCTTCAAAAACCGGACC
CTCGGGCTGATCGATGCTTCCTGGAGTGCCATTCGAATAGGGATCTATGCGCTGCATCTG
GAAAACTGGCTCCAGTATTTCCCCCTCTCCCAGATCCTCTTTGTCAGTGGTGAGCGACTC
ATTGTGGACCCCGCCGGGGAAATGGCCAAAGTACAGGATTTTCTAGGCCTCAAACGTGTT
GTGACTAAGAAGCATTTCTATTTCAACAAAACCAAGGGGTTCCCTTGCCTAAAGAAGCCA
GAAGACAGCAGTGCCCCGAGGTGCTTAGGCAAGAGCAAAGGTCGGACTCATCCTCGCATT
GACCCAGATGTCATCCACAGACTGAGGAAATTCTACAAACCCTTCAACTTGATGTTTTAC
CAAATGACTGGTCAAGATTTTCAGTGGGAACAGGAAGAGGGTGATAAATGA
|
| Protein Properties |
|---|
| Number of Residues
| 456 |
| Molecular Weight
| 49798.645 |
| Theoretical pI
| 8.684 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Heparan sulfate glucosamine 3-O-sulfotransferase 4
MARWPAPPPPPPPPPPLAAPPPPGASAKGPPARKLLFMCTLSLSVTYLCYSLLGGSGSLQ
FPLALQESPGAAAEPPPSPPPPSLLPTPVRLGAPSQPPAPPPLDNASHGEPPEPPEQPAA
PGTDGWGLPSGGGGARDAWLRTPLAPSEMITAQSALPEREAQESSTTDEDLAGRRAANGS
SERGGAVSTPDYGEKKLPQALIIGVKKGGTRALLEAIRVHPDVRAVGVEPHFFDRNYEKG
LEWYRNVMPKTLDGQITMEKTPSYFVTNEAPKRIHSMAKDIKLIVVVRNPVTRAISDYTQ
TLSKKPEIPTFEVLAFKNRTLGLIDASWSAIRIGIYALHLENWLQYFPLSQILFVSGERL
IVDPAGEMAKVQDFLGLKRVVTKKHFYFNKTKGFPCLKKPEDSSAPRCLGKSKGRTHPRI
DPDVIHRLRKFYKPFNLMFYQMTGQDFQWEQEEGDK
|
| External Links |
|---|
| GenBank ID Protein
| 46398191 |
| UniProtKB/Swiss-Prot ID
| Q9Y661 |
| UniProtKB/Swiss-Prot Entry Name
| HS3S4_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| AF105378 |
| GeneCard ID
| HS3ST4 |
| GenAtlas ID
| HS3ST4 |
| HGNC ID
| HGNC:5200 |
| References |
|---|
| General References
| - Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. BMC Genomics. 2007 Oct 31;8:399. [PubMed:17974005 ]
- Shworak NW, Liu J, Petros LM, Zhang L, Kobayashi M, Copeland NG, Jenkins NA, Rosenberg RD: Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase. Isolation, characterization, and expression of human cdnas and identification of distinct genomic loci. J Biol Chem. 1999 Feb 19;274(8):5170-84. [PubMed:9988767 ]
|
