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Showing Protein Heparan sulfate glucosamine 3-O-sulfotransferase 2 (HMDBP00352)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 3 metabolites
Identification
HMDB Protein ID HMDBP00352
Secondary Accession Numbers
  • 5588
Name Heparan sulfate glucosamine 3-O-sulfotransferase 2
Synonyms
  1. 3-OST-2
  2. Heparan sulfate 3-O-sulfotransferase 2
  3. Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2
  4. h3-OST-2
Gene Name HS3ST2
Protein Type Enzyme
Biological Properties
General Function Involved in sulfotransferase activity
Specific Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in GlcA2S-GlcNS. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.
Pathways
  • Glycosaminoglycan biosynthesis - heparan sulfate / heparin
Reactions
Phosphoadenosine phosphosulfate + [heparan sulfate]-glucosamine → Adenosine 3',5'-diphosphate + [heparan sulfate]-glucosamine 3-sulfate details
GO Classification
Biological Process
small molecule metabolic process
glycosaminoglycan biosynthetic process
carbohydrate metabolic process
circadian rhythm
Cellular Component
integral to membrane
Golgi membrane
Function
catalytic activity
transferase activity
transferase activity, transferring sulfur-containing groups
sulfotransferase activity
Molecular Function
[heparan sulfate]-glucosamine 3-sulfotransferase 2 activity
sulfotransferase activity
Cellular Location
  1. Golgi apparatus membrane
  2. Single-pass type II membrane protein (Probable)
Gene Properties
Chromosome Location 16
Locus 16p12
SNPs HS3ST2
Gene Sequence 
>1104 bp
ATGGCCTATAGGGTCCTGGGCCGCGCGGGGCCACCTCAGCCGCGGAGGGCGCGCAGGCTG
CTCTTCGCCTTCACGCTCTCGCTCTCCTGCACTTACCTGTGTTACAGCTTCCTGTGCTGC
TGCGACGACCTGGGTCGGAGCCGCCTCCTCGGCGCGCCTCGCTGCCTCCGCGGCCCCAGC
GCGGGCGGCCAGAAACTTCTCCAGAAGTCCCGCCCCTGTGATCCCTCCGGGCCGACGCCC
AGCGAGCCCAGCGCTCCCAGCGCGCCCGCCGCCGCCGTGCCCGCCCCTCGCCTCTCCGGT
TCCAACCACTCCGGCTCACCCAAGCTGGGTACCAAGCGGTTGCCCCAAGCCCTCATTGTG
GGCGTGAAGAAGGGGGGCACCCGGGCCGTGCTGGAGTTTATCCGAGTACACCCGGACGTG
CGGGCCTTGGGCACGGAACCCCACTTCTTTGACAGGAACTACGGCCGCGGGCTGGATTGG
TACAGGAGCCTGATGCCCAGGACCCTCGAGAGCCAGATCACGCTGGAGAAGACGCCCAGC
TACTTTGTCACTCAAGAGGCTCCTCGACGCATCTTCAACATGTCCCGAGACACCAAGCTG
ATCGTGGTTGTGCGGAACCCTGTGACCCGTGCCATCTCTGATTACACGCAGACACTCTCC
AAGAAGCCCGACATCCCGACCTTTGAGGGCCTCTCCTTCCGCAACCGCACCCTGGGCCTG
GTGGACGTGTCGTGGAACGCCATCCGCATCGGCATGTACGTGCTGCACCTGGAGAGCTGG
CTGCAGTACTTCCCGCTAGCTCAGATTCACTTCGTCAGTGGCGAGCGACTCATCACTGAC
CCGGCCGGCGAGATGGGGCGAGTCCAGGACTTCCTGGGCATTAAGAGATTCATCACGGAC
AAGCACTTCTATTTCAACAAGACCAAAGGATTCCCTTGCTTGAAAAAAACAGAATCGAGC
CTCCTGCCTCGATGCTTGGGCAAATCAAAAGGGAGAACTCATGTACAGATTGATCCTGAA
GTGATAGACCAGCTCCGAGAATTTTATAGACCGTATAATATCAAATTTTATGAAACCGTT
GGGCAGGACTTCAGGTGGGAATAA
Protein Properties
Number of Residues 367
Molecular Weight 41500.57
Theoretical pI 9.895
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence 
>Heparan sulfate glucosamine 3-O-sulfotransferase 2
MAYRVLGRAGPPQPRRARRLLFAFTLSLSCTYLCYSFLCCCDDLGRSRLLGAPRCLRGPS
AGGQKLLQKSRPCDPSGPTPSEPSAPSAPAAAVPAPRLSGSNHSGSPKLGTKRLPQALIV
GVKKGGTRAVLEFIRVHPDVRALGTEPHFFDRNYGRGLDWYRSLMPRTLESQITLEKTPS
YFVTQEAPRRIFNMSRDTKLIVVVRNPVTRAISDYTQTLSKKPDIPTFEGLSFRNRTLGL
VDVSWNAIRIGMYVLHLESWLQYFPLAQIHFVSGERLITDPAGEMGRVQDFLGIKRFITD
KHFYFNKTKGFPCLKKTESSLLPRCLGKSKGRTHVQIDPEVIDQLREFYRPYNIKFYETV
GQDFRWE
GenBank ID Protein 4835719
UniProtKB/Swiss-Prot ID Q9Y278
UniProtKB/Swiss-Prot Entry Name HS3S2_HUMAN
PDB IDs Not Available
GenBank Gene ID AF105374
GeneCard ID HS3ST2
GenAtlas ID HS3ST2
HGNC ID HGNC:5195
References
General References
  1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  2. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A: The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res. 2003 Oct;13(10):2265-70. Epub 2003 Sep 15. [PubMed:12975309 ]
  3. Shworak NW, Liu J, Petros LM, Zhang L, Kobayashi M, Copeland NG, Jenkins NA, Rosenberg RD: Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase. Isolation, characterization, and expression of human cdnas and identification of distinct genomic loci. J Biol Chem. 1999 Feb 19;274(8):5170-84. [PubMed:9988767 ]
  4. Liu J, Shworak NW, Sinay P, Schwartz JJ, Zhang L, Fritze LM, Rosenberg RD: Expression of heparan sulfate D-glucosaminyl 3-O-sulfotransferase isoforms reveals novel substrate specificities. J Biol Chem. 1999 Feb 19;274(8):5185-92. [PubMed:9988768 ]