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Showing Protein Ubiquitin-conjugating enzyme E2 N (HMDBP00654)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 4 metabolites
Identification
HMDB Protein ID HMDBP00654
Secondary Accession Numbers
  • 5926
Name Ubiquitin-conjugating enzyme E2 N
Synonyms
  1. Bendless-like ubiquitin-conjugating enzyme
  2. Ubc13
  3. Ubiquitin carrier protein N
  4. Ubiquitin-protein ligase N
  5. UbcH13
Gene Name UBE2N
Protein Type Enzyme
Biological Properties
General Function Involved in acid-amino acid ligase activity
Specific Function The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).
Pathways
  • protein ubiquitination
  • Ubiquitin mediated proteolysis
Reactions
Adenosine triphosphate + ubiquitin + protein lysine → Adenosine monophosphate + Pyrophosphate + protein N-ubiquityllysine details
GO Classification
Biological Process
protein K63-linked ubiquitination
DNA double-strand break processing
double-strand break repair via homologous recombination
T cell receptor signaling pathway
histone ubiquitination
nucleotide-binding oligomerization domain containing signaling pathway
positive regulation of histone modification
positive regulation of ubiquitin-protein ligase activity
regulation of histone ubiquitination
innate immune response
proteolysis
positive regulation of I-kappaB kinase/NF-kappaB cascade
positive regulation of DNA repair
cytokine-mediated signaling pathway
postreplication repair
MyD88-dependent toll-like receptor signaling pathway
Toll signaling pathway
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
positive regulation of NF-kappaB transcription factor activity
toll-like receptor 4 signaling pathway
Cellular Component
cytosol
nucleus
ubiquitin ligase complex
UBC13-MMS2 complex
UBC13-UEV1A complex
Function
catalytic activity
small conjugating protein ligase activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
acid-amino acid ligase activity
Molecular Function
ubiquitin binding
ubiquitin-protein ligase activity
ATP binding
Process
metabolic process
regulation of protein metabolic process
macromolecule metabolic process
biological regulation
regulation of biological process
regulation of metabolic process
regulation of macromolecule metabolic process
post-translational protein modification
macromolecule modification
protein modification process
Cellular Location
  1. Nucleus
  2. Cytoplasm
Gene Properties
Chromosome Location 12
Locus 12q22
SNPs UBE2N
Gene Sequence 
>459 bp
ATGGCCGGGCTGCCCCGCAGGATCATCAAGGAAACCCAGCGTTTGCTGGCAGAACCAGTT
CCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCCGTTATTTTCATGTGGTCATTGCT
GGCCCTCAGGATTCCCCCTTTGAGGGAGGGACTTTTAAACTTGAACTATTCCTTCCAGAA
GAATACCCAATGGCAGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTA
GACAAGTTGGGAAGAATATGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCACTGCAG
ATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAGTGCTCCCAATCCAGATGATCCA
TTAGCAAATGATGTAGCGGAGCAGTGGAAGACCAACGAAGCCCAAGCCATAGAAACAGCT
AGAGCATGGACTAGGCTATATGCCATGAATAATATTTAA
Protein Properties
Number of Residues 152
Molecular Weight 17137.625
Theoretical pI 6.567
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence 
>Ubiquitin-conjugating enzyme E2 N
MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPE
EYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP
LANDVAEQWKTNEAQAIETARAWTRLYAMNNI
GenBank ID Protein 12653255
UniProtKB/Swiss-Prot ID P61088
UniProtKB/Swiss-Prot Entry Name UBE2N_HUMAN
PDB IDs
GenBank Gene ID BC000396
GeneCard ID UBE2N
GenAtlas ID UBE2N
HGNC ID HGNC:12492
References
General References
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