Hmdb loader
Identification
HMDB Protein ID HMDBP01260
Secondary Accession Numbers
  • 6556
Name Homeodomain-interacting protein kinase 3
Synonyms
  1. ANPK
  2. Androgen receptor-interacting nuclear protein kinase
  3. FIST
  4. Fas-interacting serine/threonine-protein kinase
  5. Homolog of protein kinase YAK1
Gene Name HIPK3
Protein Type Enzyme
Biological Properties
General Function Involved in protein kinase activity
Specific Function Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors
Pathways Not Available
Reactions Not Available
GO Classification
Function
binding
catalytic activity
transferase activity
transferase activity, transferring phosphorus-containing groups
kinase activity
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
atp binding
protein kinase activity
protein serine/threonine kinase activity
Process
phosphorus metabolic process
phosphate metabolic process
metabolic process
cellular metabolic process
protein amino acid phosphorylation
phosphorylation
Cellular Location
  1. Nucleus
  2. Cytoplasm
Gene Properties
Chromosome Location Chromosome:1
Locus 11p13
SNPs HIPK3
Gene Sequence
>3648 bp
ATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTT
TGTAGTGTGAAGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTAT
CCACGGACCTATGTGAATGGTAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGT
GCTTTTCAGACAAAGATACCATTTAATAGACCTCGAGGACACAACTTTTCATTGCAGACA
AGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGGTCATAGCAGCTCAGGCACAG
CAAGCTCACGTGCAGGCACCTCAGATTGGGGTGTGGCGAAACAGATTGCATTTCCTAGAA
GGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCA
ATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAAT
CCAGTGACAGTTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGT
GACTATCAGTTAGTACAGCATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTT
GATTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAAT
GAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATA
GAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGA
GCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAA
AACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGG
CCCATTCTTCAACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTCAT
GCTGATCTCAAGCCAGAGAATATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTT
AAAGTAATAGACTTTGGGTCGGCCAGTCATGTATCAAAGACTGTTTGTTCAACATATCTA
CAATCTCGGTACTACAGAGCTCCAGAGATTATATTGGGGTTGCCATTTTGTGAAGCCATA
GACATGTGGTCATTGGGATGTGTGATTGCAGAATTATTTCTTGGATGGCCGCTCTACCCA
GGAGCCTTGGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTTGCCAGGAGAA
CAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTTGCAAAGAAACAGATATGTCT
CATTCTGGTTGGAGATTAAAGACATTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCT
AAAGAAGCCAGAAAATACATTTTCAACAGTCTGGATGATGTAGCGCATGTGAACACAGTG
ATGGATTTGGAAGGAAGTGATCTTTTGGCTGAGAAAGCTGATAGAAGAGAATTTGTTAGT
CTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAATTACTCCAGCTGAGACCCTG
AACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCCCTCATAGCAACCATGTAAAG
TCCTGTTTTCATATTATGGATATTTGTAAGTCCCACCTAAATTCATGTGACACAAATAAT
CACAACAAAACTTCACTTTTAAGACCAGTTGCTTCAAGCAGTACTGCTACACTGACTGCA
AATTTTACTAAAATCGGAACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTT
GTGCACCATGGAATACCTCTGCAGGCAGGAACTGCTCAGTTTGGTTGTGGTGATGCTTTT
CAGCAGACATTGATTATCTGTCCCCCAGCTATTCAAGGTATTCCTGCAACACATGGTAAA
CCCACCAGTTATTCAATAAGGGTAGATAATACAGTTCCACTTGTAACTCAGGCCCCAGCT
GTGCAGCCACTACAGATCCGACCAGGAGTTCTTTCTCAGACGTGGTCTGGTAGAACACAG
CAGATGCTGGTGCCTGCCTGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTA
ACTTCTGAGAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGATGATTTCATGC
AGCAATCATTATAACTCAGTGATGCCGCAGCCTCTTCTGACCAATCAGATAACTTTATCT
GCCCCTCAGCCAGTTAGTGTGGGGATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACC
AAGAAAAATAAACAGTGCCAGAACAGAGGTATTTTGGTAAAACTAATGGAATGGGAGCCA
GGAAGAGAGGAAATAAATGCTTTCAGTTGGAGTAATTCATTACAGAATACCAATATCCCA
CATTCAGCATTTATTTCTCCAAAGATAATTAATGGGAAAGATGTCGAGGAAGTAAGTTGT
ATAGAAACACAGGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGAAACATCT
ATCAGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGCAAACCATCATTATTGCC
GACTCCCCGAGTCCTGCAGTGAGTGTCATCACTATCAGCAGTGACACTGATGAGGAAGAG
ACTTCCCAGAGACATTCACTCAGAGAATGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAG
AGCACTTTGAATATTGATCGGATGTGTTCATTAAGTAGTCCTGATAGTACTCTGAGTACC
AGCTCCTCAGGGCAGTCCAGCCCATCCCCCTGCAAGAGACCGAATAGTATGTCAGATGAA
GAGCAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACATCTGACTCTTCCGGGCAT
GACAGTCCATTTGCAGAGAGCACTTTTGTGGAGGACACTCATGAAAACACAGAATTGGTA
TCCTCTGCTGACACAGAAACCAAGCCAGCTGTCTGTTCTGTTGTGGTGCCACCAGTGGAA
CTAGAAAATGGCTTAAATGCCGATGAGCATATGGCAAACACAGATTCTATATGCCAGCCA
TTAATAAAAGGACGATCTGCCCCTGGAAGATTAAACCAGCCTTCTGCAGTGGGTACTCGT
CAGCAAAAATTGACATCAGCATTCCAGCAGCAGCATTTGAACTTCAGTCAGGTTCAGCAC
TTTGGATCTGGGCATCAAGAGTGGAATGGAAACTTTGGGCACAGAAGACAGCAAGCTTAT
ATTCCTACTAGTGTTACCAGTAATCCATTCACTCTTTCTCATGGAAGTCCCAATCACACA
GCAGTGCATGCCCACCTGGCTGGAAATACACACCTCGGAGGACAGCCTACTCTACTTCCA
TACCCATCATCAGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGCCTCTCCG
TGTACCTCAAGACCTATGTTACAGCATCCAACTTATAATATCTCCCATCCCAGTGGCATA
GTTCACCAAGTCCCAGTGGGCTTAAATCCCCGTCTGTTACCATCCCCAACCATTCATCAG
ACTCAGTACAAACCAATCTTCCCACCACATTCTTACATTGCAGCATCACCTGCATATACT
GGATTTCCACTGAGTCCAACAAAACTCAGCCAGTATCCATATATGTGA
Protein Properties
Number of Residues 1215
Molecular Weight 133742.1
Theoretical pI 7.46
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence
>Homeodomain-interacting protein kinase 3
MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNYPRTYVNGRNFGNSHPPTKGS
AFQTKIPFNRPRGHNFSLQTSAVVLKNTAGATKVIAAQAQQAHVQAPQIGAWRNRLHFLE
GPQRCGLKRKSEELDNHSSAMQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTTGEG
DYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQI
EVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIR
PILQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCSTYL
QSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGE
QLLNVGTKSTRFFCKETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDVAHVNTV
MDLEGSDLLAEKADRREFVSLLKKMLLIDADLRITPAETLNHPFVNMKHLLDFPHSNHVK
SCFHIMDICKSHLNSCDTNNHNKTSLLRPVASSSTATLTANFTKIGTLRSQALTTSAHSV
VHHGIPLQAGTAQFGCGDAFQQTLIICPPAIQGIPATHGKPTSYSIRVDNTVPLVTQAPA
VQPLQIRPGVLSQTWSGRTQQMLVPAWQQVTPLAPATTTLTSESVAGSHRLGDWGKMISC
SNHYNSVMPQPLLTNQITLSAPQPVSVGIAHVVWPQPATTKKNKQCQNRGILVKLMEWEP
GREEINAFSWSNSLQNTNIPHSAFISPKIINGKDVEEVSCIETQDNQNSEGEARNCCETS
IRQDSDSSVSDKQRQTIIIADSPSPAVSVITISSDTDEEETSQRHSLRECKGSLDCEACQ
STLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKRPNSMSDEEQESSCDTVDGSPTSDSSGH
DSPFAESTFVEDTHENTELVSSADTETKPAVCSVVVPPVELENGLNADEHMANTDSICQP
LIKGRSAPGRLNQPSAVGTRQQKLTSAFQQQHLNFSQVQHFGSGHQEWNGNFGHRRQQAY
IPTSVTSNPFTLSHGSPNHTAVHAHLAGNTHLGGQPTLLPYPSSATLSSAAPVAHLLASP
CTSRPMLQHPTYNISHPSGIVHQVPVGLNPRLLPSPTIHQTQYKPIFPPHSYIAASPAYT
GFPLSPTKLSQYPYM
GenBank ID Protein 2627331
UniProtKB/Swiss-Prot ID Q9H422
UniProtKB/Swiss-Prot Entry Name HIPK3_HUMAN
PDB IDs Not Available
GenBank Gene ID AF004849
GeneCard ID HIPK3
GenAtlas ID HIPK3
HGNC ID HGNC:4915
References
General References
  1. Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ: Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nat Biotechnol. 2005 Jan;23(1):94-101. Epub 2004 Dec 12. [PubMed:15592455 ]
  2. Oppermann FS, Gnad F, Olsen JV, Hornberger R, Greff Z, Keri G, Mann M, Daub H: Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics. 2009 Jul;8(7):1751-64. doi: 10.1074/mcp.M800588-MCP200. Epub 2009 Apr 15. [PubMed:19369195 ]
  3. Rikova K, Guo A, Zeng Q, Possemato A, Yu J, Haack H, Nardone J, Lee K, Reeves C, Li Y, Hu Y, Tan Z, Stokes M, Sullivan L, Mitchell J, Wetzel R, Macneill J, Ren JM, Yuan J, Bakalarski CE, Villen J, Kornhauser JM, Smith B, Li D, Zhou X, Gygi SP, Gu TL, Polakiewicz RD, Rush J, Comb MJ: Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. Cell. 2007 Dec 14;131(6):1190-203. [PubMed:18083107 ]
  4. Taylor TD, Noguchi H, Totoki Y, Toyoda A, Kuroki Y, Dewar K, Lloyd C, Itoh T, Takeda T, Kim DW, She X, Barlow KF, Bloom T, Bruford E, Chang JL, Cuomo CA, Eichler E, FitzGerald MG, Jaffe DB, LaButti K, Nicol R, Park HS, Seaman C, Sougnez C, Yang X, Zimmer AR, Zody MC, Birren BW, Nusbaum C, Fujiyama A, Hattori M, Rogers J, Lander ES, Sakaki Y: Human chromosome 11 DNA sequence and analysis including novel gene identification. Nature. 2006 Mar 23;440(7083):497-500. [PubMed:16554811 ]
  5. Heibeck TH, Ding SJ, Opresko LK, Zhao R, Schepmoes AA, Yang F, Tolmachev AV, Monroe ME, Camp DG 2nd, Smith RD, Wiley HS, Qian WJ: An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells. J Proteome Res. 2009 Aug;8(8):3852-61. doi: 10.1021/pr900044c. [PubMed:19534553 ]
  6. Greenman C, Stephens P, Smith R, Dalgliesh GL, Hunter C, Bignell G, Davies H, Teague J, Butler A, Stevens C, Edkins S, O'Meara S, Vastrik I, Schmidt EE, Avis T, Barthorpe S, Bhamra G, Buck G, Choudhury B, Clements J, Cole J, Dicks E, Forbes S, Gray K, Halliday K, Harrison R, Hills K, Hinton J, Jenkinson A, Jones D, Menzies A, Mironenko T, Perry J, Raine K, Richardson D, Shepherd R, Small A, Tofts C, Varian J, Webb T, West S, Widaa S, Yates A, Cahill DP, Louis DN, Goldstraw P, Nicholson AG, Brasseur F, Looijenga L, Weber BL, Chiew YE, DeFazio A, Greaves MF, Green AR, Campbell P, Birney E, Easton DF, Chenevix-Trench G, Tan MH, Khoo SK, Teh BT, Yuen ST, Leung SY, Wooster R, Futreal PA, Stratton MR: Patterns of somatic mutation in human cancer genomes. Nature. 2007 Mar 8;446(7132):153-8. [PubMed:17344846 ]
  7. Begley DA, Berkenpas MB, Sampson KE, Abraham I: Identification and sequence of human PKY, a putative kinase with increased expression in multidrug-resistant cells, with homology to yeast protein kinase Yak1. Gene. 1997 Oct 24;200(1-2):35-43. [PubMed:9373137 ]
  8. Rochat-Steiner V, Becker K, Micheau O, Schneider P, Burns K, Tschopp J: FIST/HIPK3: a Fas/FADD-interacting serine/threonine kinase that induces FADD phosphorylation and inhibits fas-mediated Jun NH(2)-terminal kinase activation. J Exp Med. 2000 Oct 16;192(8):1165-74. [PubMed:11034606 ]
  9. Moilanen AM, Karvonen U, Poukka H, Janne OA, Palvimo JJ: Activation of androgen receptor function by a novel nuclear protein kinase. Mol Biol Cell. 1998 Sep;9(9):2527-43. [PubMed:9725910 ]
  10. Minty A, Dumont X, Kaghad M, Caput D: Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1 interaction motif. J Biol Chem. 2000 Nov 17;275(46):36316-23. [PubMed:10961991 ]
  11. Curtin JF, Cotter TG: JNK regulates HIPK3 expression and promotes resistance to Fas-mediated apoptosis in DU 145 prostate carcinoma cells. J Biol Chem. 2004 Apr 23;279(17):17090-100. Epub 2004 Feb 6. [PubMed:14766760 ]