Human Metabolome Database: Showing Protein Cytochrome P450 4F8 (HMDBP01564)

Identification Biological properties Gene properties Protein properties External links References XML Show 10 metabolites

Identification

HMDB Protein ID

HMDBP01564

Secondary Accession Numbers

6860

Name

Cytochrome P450 4F8

Synonyms

CYPIVF8

Gene Name

CYP4F8

Protein Type

Unknown

Biological Properties

General Function

Secondary metabolites biosynthesis, transport and catabolism

Specific Function

Hydroxylates arachidonic acid (20:4n-6) to (18R)-hydroxyarachidonate. Shows little activity against prostaglandin (PG) D2, PGE1, PGE2, PGF2alpha, and leukotriene B4. Catalyzes omega-2 and omega-3-hydroxylation of PGH1 and PGH2. Catalyzes epoxidation of 4,7,10,13,16,19-(Z)-docosahexaenoic acid (22:6n-3) and 7,10,13,16,19-(Z)-docosapentaenoic acid (22:5n-3) and omega-3-hydroxylation of 4,7,10,13,16-(Z)-docosapentaenoic acid (22:5n-6). Catalyzes hydroxylation of PGI2 and carbaprostacyclin.

Pathways

Acetaminophen Action Pathway
Acetylsalicylic Acid Action Pathway
Antipyrine Action Pathway
Antrafenine Action Pathway
Arachidonic acid metabolism
Arachidonic Acid Metabolism
Bromfenac Action Pathway
Carprofen Action Pathway
Celecoxib Action Pathway
Diclofenac Action Pathway
Diflunisal Action Pathway
Etodolac Action Pathway
Etoricoxib Action Pathway
Fenoprofen Action Pathway
Flurbiprofen Action Pathway
Ibuprofen Action Pathway
Indomethacin Action Pathway
Ketoprofen Action Pathway
Ketorolac Action Pathway
Leukotriene C4 Synthesis Deficiency
Lornoxicam Action Pathway
Lumiracoxib Action Pathway
Magnesium salicylate Action Pathway
Mefenamic Acid Action Pathway
Meloxicam Action Pathway
Nabumetone Action Pathway
Naproxen Action Pathway
Nepafenac Action Pathway
Oxaprozin Action Pathway
Phenylbutazone Action Pathway
Piroxicam Action Pathway
Rofecoxib Action Pathway
Salicylate-sodium Action Pathway
Salicylic Acid Action Pathway
Salsalate Action Pathway
Sulindac Action Pathway
Suprofen Action Pathway
Tenoxicam Action Pathway
Tiaprofenic Acid Action Pathway
Tolmetin Action Pathway
Trisalicylate-choline Action Pathway
Valdecoxib Action Pathway

Reactions

RH + reduced flavoprotein + Oxygen → ROH + oxidized flavoprotein + Water	details
Leukotriene B4 + NADPH + Hydrogen Ion + Oxygen → 20-Hydroxy-leukotriene B4 + NADP + Water	details
Arachidonic acid + Oxygen + NADPH + Hydrogen Ion → 20-Hydroxyeicosatetraenoic acid + NADP + Water	details

GO Classification

Biological Process
prostaglandin metabolic process
xenobiotic metabolic process
Cellular Component
endoplasmic reticulum membrane
integral to membrane
Function
ion binding
cation binding
metal ion binding
binding
catalytic activity
transition metal ion binding
electron carrier activity
iron ion binding
monooxygenase activity
heme binding
oxidoreductase activity
Molecular Function
electron carrier activity
aromatase activity
iron ion binding
heme binding
alkane 1-monooxygenase activity
Process
metabolic process
oxidation reduction

Cellular Location

Endoplasmic reticulum membrane
Single-pass membrane protein
Microsome membrane

Gene Properties

Chromosome Location

Locus

19p13.1

SNPs

CYP4F8

Gene Sequence

>1563 bp
ATGTCGCTGCTGAGCCTGTCTTGGCTGGGCCTCAGGCCGGTGGCAGCATCCCCGTGGCTG
CTCCTGCTGGTGGTCGGGGCCTCCTGGCTCCTGGCCCGCATCCTGGCCTGGACCTATGCC
TTCTATCACAACGGCCGCCGCCTCCGGTGTTTCCCGCAGCCCCGGAAACAGAACTGGTTC
TTGGGTCACCTGGGCCTGGTCACTCCCACAGAGGAGGGCTTGAGGGTCCTGACCCAGCTG
GTGGCCACCTACCCCCAGGGCTTTGTGAGGTGGTTGGGCCCCATCACTCCCATCATCAAC
TTGTGCCACCCTGACATCGTCCGATCTGTCATCAATACCTCAGATGCCATTACAGACAAG
GACATAGTCTTCTACAAGACCCTGAAGCCCTGGCTGGGGGATGGGCTCTTGTTAAGTGTT
GGTGACAAGTGGAGACACCACCGTCGCTTGCTGACGCCTGCCTTCCATTTCAACATCCTG
AAGCCCTATATAAAGATTTTCAGCAAGAGTGCAAACATCATGCATGCCAAGTGGCAACGC
CTGGCCATGGAGGGCAGCACCTGTCTGGATGTGTTTGAGCACATCAGCCTTATGACCCTG
GACAGTCTGCAGAAATGCATCTTCAGCTTTGACAGCAATTGTCAGGAGAAGCCCAGTGAA
TATATTACTGCGATCATGGAGCTCAGTGCCCTTGTAGTGAAACGGAATAACCAGTTCTTC
CGGTACAAGGACTTCCTGTACTTCCTCACTCCCTGTGGACGGCGCTTCCACAGGGCCTGC
AGACTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGCACCCTCACTAGC
CAGGGTGTTGATGACTTCCTCCAAGCCAAGGCCAAGTCCAAGACTTTGGACTTTATTGAT
GTGCTCCTGCTGAGCGAGGATAAAAATGGTAAAGAGTTGTCAGATGAGGACATAAGAGCA
GAAGCTGACACTTTCATGTTTGGAGGCCATGACACCACGGCCAGTGGCCTCTCCTGGGTC
TTGTACAACCTCGCGAGGCACCCAGAATACCAAGAACGCTGCCGGCAGGAGGTGCAAGAG
CTTCTGAAGGACCGTGAGCCTAAAGAGATTGAATGGGACGACCTGGCCCAGTTGCCCTTC
CTGACCATGTGCCTGAAGGAGAGCCTGCGGTTGCATCCCCCAATCCCTACATTCGCCCGC
GGCTGCACCCAGGACGTGGTGCTCCCAGACAGCCGAGTCATCCCCAAAGGGAATGTCTGT
AACATCAACATCTTCGCAATCCATCACAACCCCTCAGTCTGGCCAGACCCTGAGGTCTAT
GACCCCTTCCGCTTCGACCCCGAAAACGCCCAGAAGAGGTCACCTATGGCTTTTATTCCT
TTCTCGGCGGGGCCCAGGAACTGCATCGGGCAGAAGTTCGCGATGGCAGAGATGAAGGTG
GTCCTGGCGCTCACGCTGCTGCGCTTCCGCATCCTGCCCGACCACAGGGAGCCACGCAGG
ACGCCGGAGATTGTTTTGCGTGCGGAGGACGGACTTTGGCTGCGAGTAGAACCCCTGGGC
TGA

Protein Properties

Number of Residues

520

Molecular Weight

59993.995

Theoretical pI

8.429

Pfam Domain Function

p450 (PF00067 )

Signals

Not Available

Transmembrane Regions

Not Available

Protein Sequence

>Cytochrome P450 4F8
MSLLSLSWLGLRPVAASPWLLLLVVGASWLLARILAWTYAFYHNGRRLRCFPQPRKQNWF
LGHLGLVTPTEEGLRVLTQLVATYPQGFVRWLGPITPIINLCHPDIVRSVINTSDAITDK
DIVFYKTLKPWLGDGLLLSVGDKWRHHRRLLTPAFHFNILKPYIKIFSKSANIMHAKWQR
LAMEGSTCLDVFEHISLMTLDSLQKCIFSFDSNCQEKPSEYITAIMELSALVVKRNNQFF
RYKDFLYFLTPCGRRFHRACRLVHDFTDAVIQERRRTLTSQGVDDFLQAKAKSKTLDFID
VLLLSEDKNGKELSDEDIRAEADTFMFGGHDTTASGLSWVLYNLARHPEYQERCRQEVQE
LLKDREPKEIEWDDLAQLPFLTMCLKESLRLHPPIPTFARGCTQDVVLPDSRVIPKGNVC
NINIFAIHHNPSVWPDPEVYDPFRFDPENAQKRSPMAFIPFSAGPRNCIGQKFAMAEMKV
VLALTLLRFRILPDHREPRRTPEIVLRAEDGLWLRVEPLG

External Links

GenBank ID Protein

5733409

UniProtKB/Swiss-Prot ID

P98187

UniProtKB/Swiss-Prot Entry Name

CP4F8_HUMAN

PDB IDs

Not Available

GenBank Gene ID

AF133298

GeneCard ID

CYP4F8

GenAtlas ID

Not Available

HGNC ID

HGNC:2648

References

General References

Bylund J, Finnstrom N, Oliw EH: Gene expression of a novel cytochrome P450 of the CYP4F subfamily in human seminal vesicles. Biochem Biophys Res Commun. 1999 Jul 22;261(1):169-74. [PubMed:10405341 ]
Bylund J, Hidestrand M, Ingelman-Sundberg M, Oliw EH: Identification of CYP4F8 in human seminal vesicles as a prominent 19-hydroxylase of prostaglandin endoperoxides. J Biol Chem. 2000 Jul 21;275(29):21844-9. [PubMed:10791960 ]
Stark K, Torma H, Cristea M, Oliw EH: Expression of CYP4F8 (prostaglandin H 19-hydroxylase) in human epithelia and prominent induction in epidermis of psoriatic lesions. Arch Biochem Biophys. 2003 Jan 1;409(1):188-96. [PubMed:12464258 ]
Stark K, Wongsud B, Burman R, Oliw EH: Oxygenation of polyunsaturated long chain fatty acids by recombinant CYP4F8 and CYP4F12 and catalytic importance of Tyr-125 and Gly-328 of CYP4F8. Arch Biochem Biophys. 2005 Sep 15;441(2):174-81. [PubMed:16112640 ]
Stark K, Bylund J, Torma H, Sahlen G, Oliw EH: On the mechanism of biosynthesis of 19-hydroxyprostaglandins of human seminal fluid and expression of cyclooxygenase-2, PGH 19-hydroxylase (CYP4F8) and microsomal PGE synthase-1 in seminal vesicles and vas deferens. Prostaglandins Other Lipid Mediat. 2005 Jan;75(1-4):47-64. [PubMed:15789615 ]