| Identification |
|---|
| HMDB Protein ID
| HMDBP02475 |
| Secondary Accession Numbers
| |
| Name
| Leucyl-cystinyl aminopeptidase |
| Synonyms
|
- Cystinyl aminopeptidase
- IRAP
- Insulin-regulated membrane aminopeptidase
- Insulin-responsive aminopeptidase
- Leucyl-cystinyl aminopeptidase, pregnancy serum form
- OTase
- Oxytocinase
- P-LAP
- Placental leucine aminopeptidase
|
| Gene Name
| LNPEP |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Involved in proteolysis |
| Specific Function
| Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Function |
| ion binding |
| cation binding |
| metal ion binding |
| binding |
| catalytic activity |
| hydrolase activity |
| transition metal ion binding |
| zinc ion binding |
| peptidase activity |
| peptidase activity, acting on l-amino acid peptides |
| metallopeptidase activity |
| Process |
| metabolic process |
| macromolecule metabolic process |
| protein metabolic process |
| proteolysis |
|
| Cellular Location
|
- Leucyl-cystinyl aminopeptidase
- pregnancy serum form:Secreted
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:5 |
| Locus
| 5q15 |
| SNPs
| LNPEP |
| Gene Sequence
|
>3078 bp
ATGGAGCCCTTCACCAATGATCGGCTTCAGCTCCCCAGGAATATGATTGAAAACAGCATG
TTTGAGGAAGAACCAGATGTGGTGGATTTAGCCAAAGAGCCTTGTTTACATCCTCTAGAG
CCTGATGAGGTGGAATATGAGCCCCGGGGTTCCCGACTGCTGGTGCGGGGTCTTGGTGAG
CATGAGATGGAGGAGGATGAAGAGGATTATGAGTCATCAGCAAAGCTGCTGGGCATGTCC
TTCATGAATAGAAGCTCAGGCCTTCGGAACAGTGCAACTGGTTACAGGCAGAGCCCAGAT
GGGGCTTGTTCAGTACCCTCTGCAAGGACCATGGTGGTCTGTGCTTTTGTCATCGTGGTT
GCTGTTTCTGTAATCATGGTGATTTACTTACTGCCCAGATGTACCTTTACCAAAGAAGGC
TGCCATAAAAAAAACCAGTCAATTGGACTAATTCAGCCATTTGCAACAAATGGGAAATTG
TTTCCATGGGCACAGATCAGGCTTCCCACTGCCGTTGTGCCACTACGCTATGAACTCAGC
CTACACCCGAACCTAACCTCGATGACATTCAGGGGTTCTGTGACAATTTCAGTTCAGGCT
CTTCAGGTCACATGGAATATCATTCTTCATAGCACAGGTCATAATATTTCAAGAGTGACC
TTTATGTCAGCAGTTTCAAGCCAAGAAAAACAAGCTGAGATCCTGGAATATGCATATCAT
GGACAGATCGCCATTGTTGCCCCCGAAGCCCTTCTAGCAGGGCACAATTATACGTTGAAG
ATAGAGTACTCGGCAAATATATCTAGTTCTTATTATGGGTTTTATGGCTTCTCCTACACA
GATGAAAGTAATGAGAAAAAGTACTTTGCAGCAACTCAGTTTGAACCCCTGGCAGCAAGA
TCTGCTTTTCCTTGTTTTGATGAACCAGCATTTAAAGCCACTTTTATCATCAAGATCATA
AGGGATGAGCAATACACCGCTTTATCAAATATGCCTAAGAAGTCATCAGTCGTTCTAGAT
GATGGACTTGTTCAGGATGAGTTTTCTGAGAGTGTGAAGATGAGCACTTACTTGGTTGCT
TTCATTGTGGGAGAGATGAAGAACCTGAGTCAGGACGTAAATGGAACCCTGGTTTCTATA
TATGCTGTACCAGAAAAGATTGGTCAAGTTCATTATGCCTTGGAAACAACTGTGAAGCTT
CTTGAGTTTTTTCAAAACTACTTTGAAATTCAGTACCCACTTAAGAAATTGGATTTGGTG
GCTATTCCTGACTTTGAAGCAGGAGCAATGGAAAATTGGGGTTTGCTCACCTTCCGAGAG
GAGACACTTCTGTATGACAGTAACACTTCTTCAATGGCGGATAGAAAGCTGGTGACTAAA
ATCATTGCTCATGAGCTGGCCCACCAGTGGTTTGGCAATCTGGTAACAATGAAGTGGTGG
AATGACCTATGGCTAAATGAAGGTTTTGCCACTTTCATGGAGTATTTCTCTTTGGAAAAA
ATATTCAAAGAGCTTTCTAGTTATGAAGATTTCTTAGATGCTCGATTTAAAACCATGAAG
AAAGATTCCTTAAATTCATCTCATCCAATATCATCATCTGTTCAGTCTTCAGAACAAATT
GAAGAAATGTTTGATTCTCTTTCCTATTTTAAGGGATCTTCTCTCTTGTTGATGTTGAAA
ACTTACCTTAGTGAAGATGTGTTTCAACATGCTGTTGTCCTTTACCTGCATAATCACAGC
TATGCATCTATTCAAAGTGATGATCTGTGGGATAGTTTTAATGAGGTCACAAACCAAACA
CTAGATGTAAAGAGAATGATGAAAACCTGGACCCTGCAGAAAGGATTTCCTTTAGTGACT
GTTCAAAAGAAAGGAAAGGAACTTTTTATACAACAAGAGAGATTCTTTTTAAATATGAAG
CCTGAAATTCAGCCTTCAGATACAAGCTACCTGTGGCATATTCCACTATCCTATGTCACT
GAAGGAAGAAATTATTCAAAATATCAATCGGTATCATTACTGGATAAGAAATCAGGTGTC
ATCAATCTTACAGAAGAAGTGCTGTGGGTCAAAGTGAATATAAACATGAATGGTTATTAT
ATTGTACACTATGCAGATGATGATTGGGAAGCACTAATCCATCAGTTGAAAATAAATCCT
TATGTTCTGAGTGACAAAGACCGAGCCAACCTTATCAACAACATCTTTGAACTTGCAGGC
CTAGGCAAGGTACCTCTCAAGAGGGCCTTTGATTTGATTAATTATCTTGGAAATGAGAAC
CATACTGCACCCATCACCGAAGCCCTGTTTCAGACAGACCTCATCTATAACCTCCTTGAA
AAACTGGGATACATGGATCTGGCCTCAAGACTGGTGACTAGGGTATTTAAATTACTTCAA
AACCAAATTCAACAACAAACTTGGACTGATGAGGGCACTCCATCTATGCGAGAGCTTCGG
TCAGCCCTGCTAGAGTTTGCTTGCACCCACAACCTGGGGAACTGCTCTACTACTGCCATG
AAACTGTTTGATGACTGGATGGCATCCAATGGAACTCAAAGCCTACCTACTGATGTCATG
ACAACTGTGTTCAAAGTTGGAGCAAAAACTGACAAAGGCTGGTCATTCCTTTTGGGCAAA
TACATTTCTATAGGCTCTGAAGCAGAGAAGAACAAAATACTAGAAGCACTTGCCAGCTCA
GAGGATGTGCGGAAGCTTTACTGGTTAATGAAAAGTAGCCTGAATGGAGATAACTTCCGA
ACACAGAAGCTGTCTTTTATCATTAGAACAGTGGGTCGACATTTTCCTGGACACTTACTG
GCATGGGATTTTGTCAAAGAGAACTGGAATAAGCTTGTACAGAAGTTCCCTCTGGGGTCC
TATACCATACAAAATATTGTTGCTGGATCAACTTACCTGTTTTCAACAAAGACACATTTA
TCTGAGGTTCAGGCATTCTTTGAAAATCAGTCAGAGGCAACCTTCCGGCTTCGTTGTGTC
CAGGAGGCTTTGGAAGTCATTCAGTTGAATATCCAGTGGATGGAGAAGAACCTCAAAAGT
CTCACATGGTGGCTGTAG
|
| Protein Properties |
|---|
| Number of Residues
| 1025 |
| Molecular Weight
| 117348.1 |
| Theoretical pI
| 5.5 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Leucyl-cystinyl aminopeptidase
MEPFTNDRLQLPRNMIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGE
HEMEEDEEDYESSAKLLGMSFMNRSSGLRNSATGYRQSPDGACSVPSARTMVVCAFVIVV
AVSVIMVIYLLPRCTFTKEGCHKKNQSIGLIQPFATNGKLFPWAQIRLPTAVVPLRYELS
LHPNLTSMTFRGSVTISVQALQVTWNIILHSTGHNISRVTFMSAVSSQEKQAEILEYAYH
GQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYGFSYTDESNEKKYFAATQFEPLAAR
SAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEFSESVKMSTYLVA
FIVGEMKNLSQDVNGTLVSIYAVPEKIGQVHYALETTVKLLEFFQNYFEIQYPLKKLDLV
AIPDFEAGAMENWGLLTFREETLLYDSNTSSMADRKLVTKIIAHELAHQWFGNLVTMKWW
NDLWLNEGFATFMEYFSLEKIFKELSSYEDFLDARFKTMKKDSLNSSHPISSSVQSSEQI
EEMFDSLSYFKGSSLLLMLKTYLSEDVFQHAVVLYLHNHSYASIQSDDLWDSFNEVTNQT
LDVKRMMKTWTLQKGFPLVTVQKKGKELFIQQERFFLNMKPEIQPSDTSYLWHIPLSYVT
EGRNYSKYQSVSLLDKKSGVINLTEEVLWVKVNINMNGYYIVHYADDDWEALIHQLKINP
YVLSDKDRANLINNIFELAGLGKVPLKRAFDLINYLGNENHTAPITEALFQTDLIYNLLE
KLGYMDLASRLVTRVFKLLQNQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAM
KLFDDWMASNGTQSLPTDVMTTVFKVGAKTDKGWSFLLGKYISIGSEAEKNKILEALASS
EDVRKLYWLMKSSLNGDNFRTQKLSFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGS
YTIQNIVAGSTYLFSTKTHLSEVQAFFENQSEATFRLRCVQEALEVIQLNIQWMEKNLKS
LTWWL
|
| External Links |
|---|
| GenBank ID Protein
| 61742777 |
| UniProtKB/Swiss-Prot ID
| Q9UIQ6 |
| UniProtKB/Swiss-Prot Entry Name
| LCAP_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| NM_005575.2 |
| GeneCard ID
| LNPEP |
| GenAtlas ID
| LNPEP |
| HGNC ID
| HGNC:6656 |
| References |
|---|
| General References
| - Wollscheid B, Bausch-Fluck D, Henderson C, O'Brien R, Bibel M, Schiess R, Aebersold R, Watts JD: Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins. Nat Biotechnol. 2009 Apr;27(4):378-86. doi: 10.1038/nbt.1532. Epub 2009 Apr 6. [PubMed:19349973 ]
- Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [PubMed:18669648 ]
- Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM: Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks. Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5860-5. Epub 2007 Mar 26. [PubMed:17389395 ]
- Rogi T, Tsujimoto M, Nakazato H, Mizutani S, Tomoda Y: Human placental leucine aminopeptidase/oxytocinase. A new member of type II membrane-spanning zinc metallopeptidase family. J Biol Chem. 1996 Jan 5;271(1):56-61. [PubMed:8550619 ]
- Laustsen PG, Rasmussen TE, Petersen K, Pedraza-Diaz S, Moestrup SK, Gliemann J, Sottrup-Jensen L, Kristensen T: The complete amino acid sequence of human placental oxytocinase. Biochim Biophys Acta. 1997 May 2;1352(1):1-7. [PubMed:9177475 ]
- Rasmussen TE, Pedraza-Diaz S, Hardre R, Laustsen PG, Carrion AG, Kristensen T: Structure of the human oxytocinase/insulin-regulated aminopeptidase gene and localization to chromosome 5q21. Eur J Biochem. 2000 Apr;267(8):2297-306. [PubMed:10759854 ]
- Matsumoto H, Nagasaka T, Hattori A, Rogi T, Tsuruoka N, Mizutani S, Tsujimoto M: Expression of placental leucine aminopeptidase/oxytocinase in neuronal cells and its action on neuronal peptides. Eur J Biochem. 2001 Jun;268(11):3259-66. [PubMed:11389728 ]
- Falk K, Rotzschke O, Stevanovic S, Jung G, Rammensee HG: Pool sequencing of natural HLA-DR, DQ, and DP ligands reveals detailed peptide motifs, constraints of processing, and general rules. Immunogenetics. 1994;39(4):230-42. [PubMed:8119729 ]
- Tsujimoto M, Mizutani S, Adachi H, Kimura M, Nakazato H, Tomoda Y: Identification of human placental leucine aminopeptidase as oxytocinase. Arch Biochem Biophys. 1992 Feb 1;292(2):388-92. [PubMed:1731608 ]
- Albiston AL, McDowall SG, Matsacos D, Sim P, Clune E, Mustafa T, Lee J, Mendelsohn FA, Simpson RJ, Connolly LM, Chai SY: Evidence that the angiotensin IV (AT(4)) receptor is the enzyme insulin-regulated aminopeptidase. J Biol Chem. 2001 Dec 28;276(52):48623-6. Epub 2001 Nov 13. [PubMed:11707427 ]
|
