| Identification |
|---|
| HMDB Protein ID
| HMDBP03016 |
| Secondary Accession Numbers
| |
| Name
| Xylosyltransferase 1 |
| Synonyms
|
- Peptide O-xylosyltransferase 1
- XT-I
- XylT-I
- Xylosyltransferase I
|
| Gene Name
| XYLT1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in acetylglucosaminyltransferase activity |
| Specific Function
| Catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Component |
| membrane |
| cell part |
| Function |
| catalytic activity |
| transferase activity |
| acetylglucosaminyltransferase activity |
| udp-glycosyltransferase activity |
| transferase activity, transferring glycosyl groups |
|
| Cellular Location
|
- Golgi apparatus membrane
- Endoplasmic reticulum membrane
- Single-pass type II membrane protein
- Single-pass type II membrane protein
- Secreted (Probable)
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:1 |
| Locus
| 16p12.3 |
| SNPs
| XYLT1 |
| Gene Sequence
|
>2880 bp
ATGGTGGCGGCGCCGTGCGCCCGGAGGCTGGCCCGGCGCTCGCACTCGGCGCTGCTCGCG
GCGCTCACGGTGCTGCTGCTGCAGACGCTGGTCGTGTGGAATTTCAGCAGCCTCGACTCC
GGGGCCGGGGAGCGCCGCGGGGGCGCAGCGGTCGGCGGCGGGGAGCAGCCGCCCCCGGCC
CCGGCCCCGCGCCGGGAGCGCCGGGACCTGCCCGCCGAGCCGGCTGCAGCCCGAGGAGGA
GGAGGAGGCGGCGGCGGAGGAGGAGGAGGACGGGGGCCCCAGGCGCGGGCGCGGGGAGGC
GGCCCCGGAGAACCGCGGGGACAGCAGCCGGCCAGCCGGGGGGCACTGCCCGCCCGGGCT
CTGGATCCACACCCAAGTCCGCTCATCACCCTGGAGACTCAGGATGGCTACTTTTCTCAT
CGGCCGAAAGAGAAAGTGCGAACAGACAGCAACAACGAGAACTCTGTCCCCAAAGACTTT
GAGAATGTCGACAACAGCAACTTCGCACCCAGGACTCAAAAGCAGAAGCACCAGCCTGAG
TTGGCGAAGAAGCCACCGAGTAGACAGAAGGAGCTTTTGAAAAGGAAGCTGGAACAGCAG
GAGAAAGGAAAAGGACATACATTCCCTGGGAAAGGCCCCGGTGAGGTGCTGCCTCCCGGG
GACAGAGCCGCAGCCAACAGCAGCCACGGGAAGGATGTGTCCAGACCGCCTCATGCCAGG
AAAACTGGGGGCAGCTCCCCCGAGACCAAGTATGACCAGCCCCCTAAGTGTGACATCTCA
GGCAAGGAGGCCATCTCTGCCCTGTCCCGTGCTAAGTCCAAGCACTGCCGCCAGGAGATT
GGGGAGACTTACTGCCGCCACAAGTTAGGGCTGCTGATGCCTGAGAAGGTGACTCGGTTC
TGCCCCCTCGAGGGTAAAGCCAACAAGAACGTGCAGTGGGACGAGGACTCCGTGGAGTAC
ATGCCAGCCAACCCGGTCAGAATCGCCTTTGTCCTGGTGGTCCACGGCCGTGCCTCTCGG
CAGTTGCAGCGCATGTTCAAGGCCATCTACCACAAAGACCACTTCTACTACATCCACGTG
GACAAGCGCTCTAATTACCTGCATCGGCAAGTGCTCCAGGTCTCCAGGCAGTACAGCAAT
GTCCGCGTCACCCCCTGGAGAATGGCCACCATCTGGGGAGGAGCCAGCCTCCTGTCCACC
TACCTGCAGAGCATGCGGGACCTCCTGGAGATGACCGACTGGCCCTGGGACTTCTTCATC
AACCTGAGTGCGGCCGACTACCCCATCAGGACAAATGACCAGTTGGTGGCGTTTCTCTCC
CGATACCGAGATATGAATTTCTTGAAGTCACACGGCCGGGACAATGCAAGGTTCATTCGG
AAGCAGGGCCTGGATCGGCTCTTCCTGGAGTGCGACGCTCACATGTGGCGCCTGGGAGAT
CGGCGGATCCCAGAGGGCATTGCCGTGGATGGCGGTTCGGACTGGTTCCTGCTGAACCGG
AGGTTTGTAGAATATGTGACCTTCTCCACAGACGATCTGGTGACCAAGATGAAACAGTTC
TACTCCTACACCCTGCTTCCTGCTGAGTCCTTCTTCCATACGGTCCTGGAGAACAGCCCC
CACTGCGACACCATGGTGGACAACAACCTGCGCATCACCAACTGGAATCGCAAGCTGGGC
TGCAAGTGCCAGTACAAGCACATCGTGGACTGGTGCGGCTGCTCCCCCAATGACTTCAAG
CCGCAGGACTTCCACCGCTTCCAGCAGACAGCCCGGCCTACCTTCTTTGCCCGCAAGTTT
GAAGCCGTGGTGAATCAGGAAATCATTGGGCAGCTGGACTATTACCTGTACGGGAACTAC
CCTGCAGGTACCCCGGGCCTGCGCTCCTACTGGGAGAATGTCTACGATGAGCCTGACGGC
ATCCACAGCCTGAGCGACGTGACACTCACCTTGTACCACTCCTTTGCCCGCCTGGGTCTT
CGACGGGCCGAGACGTCCCTGCACACGGATGGGGAGAACAGCTGCCGATACTACCCAATG
GGCCACCCAGCATCTGTGCACCTCTACTTCCTTGCTGACCGCTTCCAGGGCTTTCTGATC
AAGCATCATGCTACCAATCTGGCTGTGAGCAAACTAGAGACTCTGGAGACCTGGGTGATG
CCGAAAAAAGTCTTCAAGATCGCAAGCCCACCCAGTGACTTTGGGAGGCTTCAGTTTTCC
GAGGTCGGCACTGACTGGGATGCCAAGGAGAGGCTATTCCGCAACTTTGGGGGTCTTCTG
GGGCCCATGGATGAGCCGGTGGGTATGCAGAAGTGGGGGAAGGGACCTAATGTGACCGTG
ACCGTCATTTGGGTGGATCCCGTCAATGTCATCGCAGCCACCTACGACATCCTCATTGAG
TCCACTGCCGAATTCACACACTACAAGCCCCCTTTGAACTTGCCCCTGAGGCCTGGGGTC
TGGACAGTGAAAATTCTCCACCACTGGGTGCCAGTTGCAGAGACCAAATTCCTCGTTGCG
CCTCTGACCTTCTCGAACAGGCAGCCCATCAAACCTGAGGAGGCACTGAAGCTGCACAAT
GGGCCCCTCCGCAATGCCTACATGGAGCAGAGCTTCCAGAGCCTAAACCCCGTCCTCAGC
CTGCCCATCAACCCCGCCCAGGTGGAACAGGCACGGAGGAACGCAGCCTCCACGGGCACA
GCGCTGGAGGGATGGCTGGACTCGTTGGTGGGCGGGATGTGGACTGCCATGGACATCTGT
GCCACGGGCCCCACAGCCTGCCCGGTCATGCAGACCTGCAGCCAGACGGCCTGGAGCTCC
TTCAGCCCTGACCCCAAGTCGGAGCTGGGGGCAGTCAAACCTGATGGCCGGCTCAGGTAG
|
| Protein Properties |
|---|
| Number of Residues
| 959 |
| Molecular Weight
| 107568.4 |
| Theoretical pI
| 9.61 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Xylosyltransferase 1
MVAAPCARRLARRSHSALLAALTVLLLQTLVVWNFSSLDSGAGERRGGAAVGGGEQPPPA
PAPRRERRDLPAEPAAARGGGGGGGGGGGGRGPQARARGGGPGEPRGQQPASRGALPARA
LDPHPSPLITLETQDGYFSHRPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPE
LAKKPPSRQKELLKRKLEQQEKGKGHTFPGKGPGEVLPPGDRAAANSSHGKDVSRPPHAR
KTGGSSPETKYDQPPKCDISGKEAISALSRAKSKHCRQEIGETYCRHKLGLLMPEKVTRF
CPLEGKANKNVQWDEDSVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHV
DKRSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFI
NLSAADYPIRTNDQLVAFLSRYRDMNFLKSHGRDNARFIRKQGLDRLFLECDAHMWRLGD
RRIPEGIAVDGGSDWFLLNRRFVEYVTFSTDDLVTKMKQFYSYTLLPAESFFHTVLENSP
HCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFHRFQQTARPTFFARKF
EAVVNQEIIGQLDYYLYGNYPAGTPGLRSYWENVYDEPDGIHSLSDVTLTLYHSFARLGL
RRAETSLHTDGENSCRYYPMGHPASVHLYFLADRFQGFLIKHHATNLAVSKLETLETWVM
PKKVFKIASPPSDFGRLQFSEVGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGPNVTV
TVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVA
PLTFSNRQPIKPEEALKLHNGPLRNAYMEQSFQSLNPVLSLPINPAQVEQARRNAASTGT
ALEGWLDSLVGGMWTAMDICATGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR
|
| External Links |
|---|
| GenBank ID Protein
| 28269693 |
| UniProtKB/Swiss-Prot ID
| Q86Y38 |
| UniProtKB/Swiss-Prot Entry Name
| XYLT1_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| NM_022166.3 |
| GeneCard ID
| XYLT1 |
| GenAtlas ID
| XYLT1 |
| HGNC ID
| HGNC:15516 |
| References |
|---|
| General References
| - Muller S, Schottler M, Schon S, Prante C, Brinkmann T, Kuhn J, Gotting C, Kleesiek K: Human xylosyltransferase I: functional and biochemical characterization of cysteine residues required for enzymic activity. Biochem J. 2005 Mar 1;386(Pt 2):227-36. [PubMed:15461586 ]
- Gotting C, Kuhn J, Zahn R, Brinkmann T, Kleesiek K: Molecular cloning and expression of human UDP-d-Xylose:proteoglycan core protein beta-d-xylosyltransferase and its first isoform XT-II. J Mol Biol. 2000 Dec 8;304(4):517-28. [PubMed:11099377 ]
- Kuhn J, Gotting C, Schnolzer M, Kempf T, Brinkmann T, Kleesiek K: First isolation of human UDP-D-xylose: proteoglycan core protein beta-D-xylosyltransferase secreted from cultured JAR choriocarcinoma cells. J Biol Chem. 2001 Feb 16;276(7):4940-7. Epub 2000 Nov 21. [PubMed:11087729 ]
- Gotting C, Sollberg S, Kuhn J, Weilke C, Huerkamp C, Brinkmann T, Krieg T, Kleesiek K: Serum xylosyltransferase: a new biochemical marker of the sclerotic process in systemic sclerosis. J Invest Dermatol. 1999 Jun;112(6):919-24. [PubMed:10383739 ]
- Gotting C, Kuhn J, Brinkmann T, Kleesiek K: Xylosyltransferase activity in seminal plasma of infertile men. Clin Chim Acta. 2002 Mar;317(1-2):199-202. [PubMed:11814476 ]
- Gotting C, Muller S, Schottler M, Schon S, Prante C, Brinkmann T, Kuhn J, Kleesiek K: Analysis of the DXD motifs in human xylosyltransferase I required for enzyme activity. J Biol Chem. 2004 Oct 8;279(41):42566-73. Epub 2004 Aug 4. [PubMed:15294915 ]
|
