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Showing Protein Carbohydrate sulfotransferase 5 (HMDBP03239)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 1 metabolite
Identification
HMDB Protein ID HMDBP03239
Secondary Accession Numbers
  • 8811
Name Carbohydrate sulfotransferase 5
Synonyms
  1. GST4-alpha
  2. Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha
  3. GlcNAc6ST-3
  4. Gn6st-3
  5. I-GlcNAc6ST
  6. Intestinal GlcNAc-6-sulfotransferase
  7. Intestinal N-acetylglucosamine-6-O-sulfotransferase
  8. N-acetylglucosamine 6-O-sulfotransferase 3
  9. hIGn6ST
Gene Name CHST5
Protein Type Enzyme
Biological Properties
General Function Involved in sulfotransferase activity
Specific Function Catalyzes the transfer of sulfate to position 6 of non- reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N- acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for L-selectin. Its substrate specificity may be influenced by its subcellular location
Pathways Not Available
Reactions Not Available
GO Classification
Component
golgi membrane
membrane
cell part
organelle membrane
Function
catalytic activity
transferase activity
transferase activity, transferring sulfur-containing groups
sulfotransferase activity
Process
metabolic process
primary metabolic process
carbohydrate metabolic process
Cellular Location
  1. Golgi apparatus membrane
  2. Single-pass type II membrane protein (Probable)
Gene Properties
Chromosome Location Chromosome:1
Locus 16q22.3
SNPs CHST5
Gene Sequence 
>1236 bp
ATGGGCATGAGGGCCCGGGTCCCTAAAGTTGCCCACTCCACCCGAAGGCCCCCAGCCGCC
CGCATGTGGCTGCCACGGTTCTCCAGCAAGACAGTGACAGTGCTCCTCCTGGCACAGACC
ACCTGCCTCCTGCTCTTCATCATCTCCCGGCCAGGGCCCTCATCCCCAGCCGGCGGCGAG
GATCGTGTGCACGTGCTGGTGCTGTCCTCGTGGCGCTCGGGCTCATCCTTCTTGGGCCAG
CTCTTCAGCCAGCACCCCGACGTCTTCTACCTGATGGAGCCCGCGTGGCATGTGTGGACC
ACCCTGTCGCAGGGCAGCGCGGCAACGCTGCACATGGCCGTGCGCGACCTGATGCGCTCT
ATCTTTTTGTGCGACATGGACGTGTTTGATGCCTACATGCCACAGAGCCGAAACCTGTCC
GCCTTTTTCAACTGGGCAACGAGCCGCGCGCTGTGCTCGCCGCCCGCCTGCAGCGCCTTT
CCCCGAGGCACCATCAGCAAGCAGGACGTATGCAAGACACTGTGCACGCGGCAGCCATTC
AGCCTGGCCCGGGAGGCCTGCCGCTCCTACAGCCACGTGGTGCTCAAGGAGGTGCGCTTC
TTCAACCTGCAGGTGCTCTACCCGCTGCTCAGCGACCCCGCGCTCAACCTGCGCATCGTG
CACCTGGTGCGCGACCCGCGGGCCGTGCTGCGCTCCCGGGAGGCGGCGGGCCCGATACTG
GCACGCGACAACGGCATCGTGCTGGGCACCAACGGCAAGTGGGTGGAGGCCGACCCTCAC
CTGCGCCTGATTCGCGAGGTGTGCCGCAGCCACGTGCGCATCGCCGAGGCCGCCACACTC
AAGCCGCCACCCTTCCTGCGCGGCCGCTACCGCCTGGTGCGCTTCGAGGACCTGGCGCGG
GAGCCGCTGGCAGAGATCCGCGCACTCTACGCCTTCACCGGCCTGACCCTCACGCCACAG
CTCGAGGCCTGGATCCACAACATCACCCACGGGTCGGGGATCGGCAAGCCAATCGAGGCC
TTCCATACTTCGTCTAGGAATGCGCGCAACGTCTCCCAGGCCTGGCGCCACGCGTTGCCC
TTCACTAAGATCCTGCGCGTGCAGGAGGTGTGCGCCGGCGCGCTGCAGCTGCTGGGCTAC
CGGCCTGTGTACTCTGCGGACCAGCAGCGTGACCTCACCCTGGATCTGGTGCTGCCACGA
GGCCCAGACCACTTCAGCTGGGCATCGCCTGACTGA
Protein Properties
Number of Residues 411
Molecular Weight 46160.0
Theoretical pI 10.5
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • 31-48
Protein Sequence 
>Carbohydrate sulfotransferase 5
MGMRARVPKVAHSTRRPPAARMWLPRFSSKTVTVLLLAQTTCLLLFIISRPGPSSPAGGE
DRVHVLVLSSWRSGSSFLGQLFSQHPDVFYLMEPAWHVWTTLSQGSAATLHMAVRDLMRS
IFLCDMDVFDAYMPQSRNLSAFFNWATSRALCSPPACSAFPRGTISKQDVCKTLCTRQPF
SLAREACRSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAVLRSREAAGPIL
ARDNGIVLGTNGKWVEADPHLRLIREVCRSHVRIAEAATLKPPPFLRGRYRLVRFEDLAR
EPLAEIRALYAFTGLTLTPQLEAWIHNITHGSGIGKPIEAFHTSSRNARNVSQAWRHALP
FTKILRVQEVCAGALQLLGYRPVYSADQQRDLTLDLVLPRGPDHFSWASPD
GenBank ID Protein 21362052
UniProtKB/Swiss-Prot ID Q9GZS9
UniProtKB/Swiss-Prot Entry Name CHST5_HUMAN
PDB IDs Not Available
GenBank Gene ID NM_024533.3
GeneCard ID CHST5
GenAtlas ID CHST5
HGNC ID HGNC:1973
References
General References
  1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  2. Akama TO, Nishida K, Nakayama J, Watanabe H, Ozaki K, Nakamura T, Dota A, Kawasaki S, Inoue Y, Maeda N, Yamamoto S, Fujiwara T, Thonar EJ, Shimomura Y, Kinoshita S, Tanigami A, Fukuda MN: Macular corneal dystrophy type I and type II are caused by distinct mutations in a new sulphotransferase gene. Nat Genet. 2000 Oct;26(2):237-41. [PubMed:11017086 ]
  3. Bartes A, Bhakta S, Hemmerich S: Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta). Biochem Biophys Res Commun. 2001 Apr 13;282(4):928-33. [PubMed:11352640 ]
  4. Akama TO, Misra AK, Hindsgaul O, Fukuda MN: Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate. J Biol Chem. 2002 Nov 8;277(45):42505-13. Epub 2002 Sep 5. [PubMed:12218059 ]
  5. Lee JK, Bhakta S, Rosen SD, Hemmerich S: Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue. Biochem Biophys Res Commun. 1999 Sep 24;263(2):543-9. [PubMed:10491328 ]
  6. Seko A, Nagata K, Yonezawa S, Yamashita K: Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma. Glycobiology. 2002 Jun;12(6):379-88. [PubMed:12107080 ]
  7. Lee JK, Bistrup A, van Zante A, Rosen SD: Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications. Glycobiology. 2003 Apr;13(4):245-54. Epub 2002 Nov 26. [PubMed:12626414 ]
  8. de Graffenried CL, Bertozzi CR: Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis. J Biol Chem. 2003 Oct 10;278(41):40282-95. Epub 2003 Jul 10. [PubMed:12855678 ]