| Identification |
|---|
| HMDB Protein ID
| HMDBP07654 |
| Secondary Accession Numbers
| |
| Name
| Double C2-like domain-containing protein beta |
| Synonyms
|
- Doc2-beta
|
| Gene Name
| DOC2B |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Involved in transporter activity |
| Specific Function
| Calcium sensor which positively regulates SNARE- dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Component |
| cytoplasmic vesicle |
| cytoplasmic membrane-bounded vesicle |
| coated vesicle |
| clathrin-coated vesicle |
| synaptic vesicle |
| membrane |
| cell part |
| organelle |
| vesicle |
| Function |
| transporter activity |
| Process |
| establishment of localization |
| transport |
|
| Cellular Location
|
- Cell membrane
- Cytoplasm
- Peripheral membrane protein
- Cytoplasmic granule
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:1 |
| Locus
| 17p13.3 |
| SNPs
| DOC2B |
| Gene Sequence
|
>1239 bp
ATGACCCTCCGGCGGCGCGGGGAGAAGGCGACCATCAGCATCCAGGAGCATATGGCCATC
GACGTGTGCCCCGGCCCCATCCGTCCCATCAAGCAGATCTCCGACTACTTCCCCCGCTTC
CCGCGGGGCCTGCCCCCGGACGCCGGGCCCCGAGCCGCTGCACCCCCGGACGCCCCCGCG
CGCCCGGCTGTGGCCGGTGCCGGCCGCCGCAGCCCCTCCGACGGCGCCCGCGAGGACGAC
GAGGATGTGGACCAGCTCTTCGGAGCCTACGGCTCCAGCCCGGGCCCCAGCCCGGGTCCC
AGCCCCGCGCGGCCGCCAGCCAAGCCGCCGGAGGACGAGCCGGACGCCGACGGCTACGAG
TCGGACGACTGCACTGCCCTGGGCACGCTGGACTTCAGCCTGCTGTATGACCAGGAGAAC
AACGCCCTCCACTGCACCATCACCAAGGCCAAGGGCCTGAAGCCAATGGACCACAATGGG
CTGGCAGACCCCTACGTCAAGCTGCACCTGCTGCCAGGAGCCAGTAAGGCAAATAAGCTC
AGAACAAAAACTCTCCGTAACACTCTGAACCCCACATGGAACGAGACCCTCACTTACTAC
GGGATCACAGATGAAGACATGATCCGCAAGACCCTGCGGATCTCTGTGTGTGACGAGGAC
AAATTCCGGCACAATGAGTTCATCGGGGAGACACGTGTGCCCCTGAAGAAGCTGAAACCC
AACCACACCAAGACCTTCAACATCTGCCTGGAGAAGCAGCTGCCGGTGGACAAGACTGAA
GACAAGTCCCTGGAGGAGCGGGGCCGCATCCTCATCTCCCTCAAGTACAGCTCACAGAAG
CAAGGCCTGCTGGTAGGCATCGTGCGGTGCGCCCACCTGGCCGCCATGGACGCCAACGGC
TACTCGGACCCCTACGTGAAAACATACCTGAGGCCAGATGTGGACAAGAAATCCAAACAT
AAGACAGCGGTGAAGAAAAAAACCCTGAACCCGGAGTTTAATGAGGAGTTCTGTTACGAG
ATCAAGCATGGGGACCTGGCCAAGAAGTCCCTGGAGGTCACCGTTTGGGATTACGACATT
GGAAAATCCAACGATTTCATTGGTGGTGTGGTTCTGGGCATCCACGCCAAGGGGGAGCGC
CTGAAGCACTGGTTTGACTGCCTGAAGAACAAGGACAAGCGCATCGAGCGCTGGCACACG
CTCACCAGCGAGCTCCCAGGGGCTGTGCTCAGCGACTGA
|
| Protein Properties |
|---|
| Number of Residues
| 412 |
| Molecular Weight
| 45948.7 |
| Theoretical pI
| 8.2 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Double C2-like domain-containing protein beta
MTLRRRGEKATISIQEHMAIDVCPGPIRPIKQISDYFPRFPRGLPPDAGPRAAAPPDAPA
RPAVAGAGRRSPSDGAREDDEDVDQLFGAYGSSPGPSPGPSPARPPAKPPEDEPDADGYE
SDDCTALGTLDFSLLYDQENNALHCTITKAKGLKPMDHNGLADPYVKLHLLPGASKANKL
RTKTLRNTLNPTWNETLTYYGITDEDMIRKTLRISVCDEDKFRHNEFIGETRVPLKKLKP
NHTKTFNICLEKQLPVDKTEDKSLEERGRILISLKYSSQKQGLLVGIVRCAHLAAMDANG
YSDPYVKTYLRPDVDKKSKHKTAVKKKTLNPEFNEEFCYEIKHGDLAKKSLEVTVWDYDI
GKSNDFIGGVVLGIHAKGERLKHWFDCLKNKDKRIERWHTLTSELPGAVLSD
|
| External Links |
|---|
| GenBank ID Protein
| 1235722 |
| UniProtKB/Swiss-Prot ID
| Q14184 |
| UniProtKB/Swiss-Prot Entry Name
| DOC2B_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| D70830 |
| GeneCard ID
| DOC2B |
| GenAtlas ID
| DOC2B |
| HGNC ID
| HGNC:2986 |
| References |
|---|
| General References
| - Sakaguchi G, Orita S, Maeda M, Igarashi H, Takai Y: Molecular cloning of an isoform of Doc2 having two C2-like domains. Biochem Biophys Res Commun. 1995 Dec 26;217(3):1053-61. [PubMed:8554557 ]
- Nagano F, Orita S, Sasaki T, Naito A, Sakaguchi G, Maeda M, Watanabe T, Kominami E, Uchiyama Y, Takai Y: Interaction of Doc2 with tctex-1, a light chain of cytoplasmic dynein. Implication in dynein-dependent vesicle transport. J Biol Chem. 1998 Nov 13;273(46):30065-8. [PubMed:9804756 ]
- Ke B, Oh E, Thurmond DC: Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis. J Biol Chem. 2007 Jul 27;282(30):21786-97. Epub 2007 Jun 4. [PubMed:17548353 ]
|
