| Identification |
|---|
| HMDB Protein ID
| HMDBP08170 |
| Secondary Accession Numbers
| |
| Name
| Rap guanine nucleotide exchange factor 6 |
| Synonyms
|
- PDZ domain-containing guanine nucleotide exchange factor 2
- PDZ-GEF2
- RA-GEF-2
|
| Gene Name
| RAPGEF6 |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Involved in protein binding |
| Specific Function
| Guanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Component |
| cell part |
| intracellular |
| Function |
| binding |
| enzyme regulator activity |
| protein binding |
| nucleoside-triphosphatase regulator activity |
| gtpase regulator activity |
| guanyl-nucleotide exchange factor activity |
| Process |
| biological regulation |
| regulation of biological process |
| regulation of cellular process |
| signal transduction |
| intracellular signal transduction |
| small gtpase mediated signal transduction |
| regulation of cell communication |
| regulation of signal transduction |
| regulation of small gtpase mediated signal transduction |
|
| Cellular Location
|
- Cell membrane
- Cytoplasm
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:5 |
| Locus
| 5q31.1 |
| SNPs
| RAPGEF6 |
| Gene Sequence
|
>4806 bp
ATGAACTCACCCGTGGACCCTGGCGCTAGGCAGGCGTTGAGGAAGAAGCCACCCGAGCGG
ACTCCCGAGGACTTAAATACTATTTATTCTTATCTTCATGGAATGGAAATATTATCAAAT
CTCAGGGAACATCAGCTTAGATTAATGTCTGCAAGAGCACGCTATGAGAGATACAGTGGC
AATCAGGTTCTCTTTTGTTCAGAAACGATTGCCAGATGTTGGTATATCCTACTTTCTGGA
TCTGTGCTTGTGAAAGGCTCCATGGTCTTGCCTCCTTGCAGTTTTGGTAAGCAGTTTGGA
GGAAAAAGAGGATGTGATTGTCTTGTATTAGAGCCTTCAGAAATGATTGTGGTAGAGAAT
GCCAAAGATAATGAAGATAGTATTCTACAAAGAGAAATTCCTGCCAGACAATCCCGAAGA
AGATTTCGGAAAATTAACTATAAAGGAGAGCGCCAAACCATTACTGATGATGTGGAGGTT
AACAGCTATCTTTCTCTTCCAGCTGATCTTACCAAGATGCATCTCACAGAAAACCCTCAT
CCACAGGTGACTCATGTGTCTTCTAGTCAGTCTGGTTGTAGCATTGCCAGTGACTCTGGA
AGCAGCAGTTTATCTGATATCTATCAGGCTACGGAGAGTGAGGTAGGAGATGTAGATTTG
ACACGTCTTCCAGAAGGACCTGTTGATTCTGAGGATGACGAAGAGGAAGATGAAGAGATT
GATCGAACAGATCCATTGCAGGGGCGAGATCTTGTTCGAGAATGTCTTGAAAAAGAACCT
GCAGACAAAACTGATGATGACATTGAACAATTGCTGGAGTTTATGCACCAGCTCCCTGCA
TTTGCAAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCAGTGATGATTTTTGAAGTG
GTAGAGCAGGCTGGAGCTATTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTT
ATTTTAAACGGCACTGTGGAAATCAGTCATCCAGATGGAAAAGTTGAAAATTTGTTTATG
GGAAATAGTTTTGGAATTACTCCCACTCTGGATAAGCAGTACATGCATGGAATTGTCAGG
ACTAAAGTAGATGATTGTCAGTTTGTCTGCATAGCCCAGCAAGATTATTGGAGAATTTTA
AACCATGTGGAAAAAAATACCCATAAAGTTGAGGAAGAGGGAGAAATTGTTATGGTACAT
GAGCATCGGGAACTAGACCGGAGTGGAACCAGGAAAGGACACATTGTGATCAAGGCAACA
CCTGAGCGTCTCATAATGCATTTAATAGAAGAACATTCCATCGTGGATCCAACTTATATA
GAAGATTTTCTATTAACTTACAGGACATTTCTTGAAAGTCCTTTGGATGTTGGGATCAAA
CTATTGGAATGGTTTAAGATCGACAGCTTAAGAGATAAGGTGACACGGATTGTATTATTA
TGGGTAAATAATCATTTTAATGATTTTGAAGGTGACCCTGCTATGACTCGATTTCTAGAG
GAATTTGAAAAAAATCTGGAAGATACAAAGATGAATGGTCATCTCCGGTTATTGAATATT
GCCTGTGCTGCAAAGGCTAAGTGGAGACAGGTTGTGCTGCAAAAGGCTTCCCGCGAGTCC
CCTCTACAATTCAGCCTTAATGGAGGGAGTGAGAAGGGATTTGGTATTTTTGTTGAAGGA
GTAGAACCTGGTAGCAAAGCTGCTGATTCAGGACTGAAACGTGGTGATCAGATTATGGAA
GTAAATGGACAAAACTTTGAGAATATTACATTTATGAAAGCCGTTGAAATTTTGAGGAAT
AATACTCATCTTGCACTTACTGTGAAGACCAACATTTTTGTGTTCAAAGAGTTACTTTTT
AGGACTGAACAAGAGAAATCTGGTGTTCCTCATATTCCCAAAATTGCTGAAAAAAAAAGT
AATCGCCATTCTATCCAGCATGTGCCAGGAGATATTGAACAGACATCACAGGAGAAAGGA
AGTAAGAAAGTTAAAGCAAATACTGTTTCAGGTGGAAGAAACAAAATCAGGAAGATTTTG
GATAAAACACGATTTAGTATCTTGCCTCCAAAGCTATTTAGTGATGGAGGCCTAAGCCAA
TCACAAGATGACAGCATTGTGGGAACAAGGCACTGTAGGCATAGTCTGGCTATAATGCCC
ATCCCTGGAACACTCTCATCCAGCAGCCCTGATCTCCTGCAGCCTACCACCAGTATGTTG
GATTTTTCCAATCCTTCAGATATCCCTGATCAAGTTATAAGAGTTTTCAAAGTGGATCAG
CAAAGTTGCTACATTATCATCAGTAAAGACACCACAGCTAAAGAAGTAGTTTTTCATGCT
GTTCATGAATTTGGTTTGACCGGTGCATCCGACACATATTCTCTCTGTGAAGTTTCTGTT
ACTCCTGAGGGTGTCATAAAACAGAGAAGACTTCCAGATCAGTTCTCCAAATTAGCTGAT
AGAATTCAACTCAATGGAAGGTATTACTTAAAAAATAACATGGAAACAGAAACCTTATGT
TCAGATGAAGATGCTCAAGAACTAGTTAAGGAAAGCCAGCTATCCATGCTGCAGCTCAGT
ACCATTGAGGTGGCCACCCAGCTGTCAATGAGGGACTTTGATTTGTTTCGTAATATTGAA
CCGACTGAGTACATCGATGACCTTTTTAAGTTAAATTCCAAAACAGGAAATACTCATTTG
AAGAGGTTTGAGGACATTGTAAACCAAGAGACATTCTGGGTTGCCTCAGAAATTTTAACT
GAAGCAAATCAGCTCAAACGAATGAAGATTATTAAGCATTTTATTAAAATTGCACTTCAT
TGTCGAGAATGTAAGAACTTCAATTCCATGTTTGCAATAATAAGTGGCTTGAACCTGGCA
TCTGTAGCAAGACTCAGAGGAACTTGGGAAAAGTTACCAAGCAAATACGAGAAACATCTT
CAAGATCTACAAGACATTTTTGATCCATCTAGAAACATGGCAAAGTATAGAAATATTCTT
AGTAGTCAAAGTATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATG
ACATTTCTACATGAAGGAAATGACTCCAAAGTAGATGGTTTAGTAAACTTTGAGAAGTTA
AGAATGATTTCCAAGGAAATCCGCCAAGTTGTTCGAATGACTTCTGCTAACATGGACCCA
GCTATGATGTTTCGACAGAGGTCACTGAGTCAAGGAAGCACAAATTCAAACATGCTGGAT
GTTCAGGGAGGTGCTCACAAAAAAAGGGCACGCCGCAGCTCTCTGCTTAATGCCAAGAAG
CTATATGAGGATGCCCAAATGGCAAGGAAGGTGAAGCAGTATCTTTCCAGTCTCGATGTA
GAGACAGATGAGGAGAAGTTCCAGATGATGTCATTACAGTGGGAGCCTGCATATGGTACC
TTGACCAAGAATTTAAGTGAGAAAAGATCAGCCAAATCATCTGAAATGTCTCCAGTGCCT
ATGAGGTCAGCTGGCCAAACAACTAAAGCCCACTTGCATCAACCCCACAGAGTAAGCCAG
GTGCTTCAGGTGCCAGCTGTTAATTTGCACCCCATCAGGAAGAAGGGACAAACAAAAGAC
CCTGCACTGAATACAAGTTTACCTCAGAAAGTTTTAGGAACAACTGAAGAAATAAGTGGT
AAGAAGCATACAGAAGACACTATTTCTGTGGCGTCATCTTTACATTCTAGTCCTCCTGCA
TCTCCTCAAGGCTCCCCTCACAAAGGTTACACACTTATTCCATCAGCTAAATCTGACAAC
TTGTCTGACTCCAGCCATAGTGAGATTTCTTCACGGTCCAGCATCGTGAGCAATTGTTCT
GTTGACTCCATGTCTGCAGCTCTACAGGATGAACGGTGTTCCTCTCAGGCCCTGGCAGTC
CCTGAATCCACTGGGGCATTGGAAAAGACAGAGCACGCTTCAGGGATAGGAGATCATAGT
CAACATGGCCCTGGGTGGACACTCTTGAAGCCATCTCTAATCAAGTGTTTAGCTGTCTCA
TCGTCTGTGAGCAATGAAGAGATTTCTCAAGAGCATATCATTATAGAAGCAGCTGACAGT
GGTCGTGGAAGTTGGACTTCGTGTTCAAGCAGCTCCCATGACAACTTCCAAAGCCTTCCA
AACCCAAAAAGCTGGGATTTTTTGAACTCTTACAGACATACCCATTTGGATGACCCCATT
GCTGAAGTTGAACCCACTGACTCTGAGCCCTATTCCTGTTCTAAAAGCTGCTCTAGAACT
TGTGGGCAGTGTAAAGGAAGCCTAGAGAGAAAGAGTTGGACCTCCTCCAGTTCTCTGTCT
GACACGTATGAACCAAACTATGGGACAGTTAAACAGAGAGTATTGGAGAGCACCCCAGCT
GAGTCATCTGAAGGCTTGGACCCCAAGGATGCCACTGACCCAGTTTATAAAACTGTCACT
TCAAGTACAGAAAAGGGCTTGATTGTGTACTGTGTCACCTCACCCAAGAAGGACGATAGG
TATAGGGAGCCACCTCCCACTCCTCCAGGATATTTGGGGATTTCTTTAGCGGACCTAAAG
GAAGGACCCCACACACACCTAAAACCTCCAGATTATAGTGTGGCAGTGCAGAGGTCAAAG
ATGATGCATAACAGCCTCTCTAGACTGCCACCAGCTTCTCTCAGTAGCAACCTCGTGGCC
TGTGTTCCATCGAAGATTGTAACTCAGCCTCAGAGGCATAATTTGCAGCCATTCCATCCT
AAACTAGGAGATGTGACTGATGCAGATAGCGAAGCAGATGAAAATGAACAAGTTTCAGCA
GTCTAG
|
| Protein Properties |
|---|
| Number of Residues
| 1601 |
| Molecular Weight
| 179405.2 |
| Theoretical pI
| 6.4 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Rap guanine nucleotide exchange factor 6
MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSG
NQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVEN
AKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPH
PQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEI
DRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEV
VEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVR
TKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKAT
PERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLL
WVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRES
PLQFSLNGGSEKGFGIFVEGVEPGSEAADSGLKRGDQIMEVNGQNFENITFMKAVEILRN
NTHLALTVKTNIFVFKELPFRTEQEKSGVPHIPKIAKKKSNRHSIQHVPGDIEQTSQEKG
SKKVKANTASGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMP
IPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHA
VHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLC
SDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHL
KRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLA
SVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDM
TFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLD
VQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGT
LTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKD
PALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDN
LSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHS
QHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLP
NPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLS
DTYEPNYGTVKRRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIVYCVTSPKKDDR
YREPPPTPPGYLGISLADLKEGPHTHLKPPDYSVAVQRSKMMHNSLSRLPPASLSSNLVA
CVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV
|
| External Links |
|---|
| GenBank ID Protein
| 256600194 |
| UniProtKB/Swiss-Prot ID
| Q8TEU7 |
| UniProtKB/Swiss-Prot Entry Name
| RPGF6_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| NM_016340.5 |
| GeneCard ID
| RAPGEF6 |
| GenAtlas ID
| RAPGEF6 |
| HGNC ID
| HGNC:20655 |
| References |
|---|
| General References
| - Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [PubMed:18669648 ]
- Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. [PubMed:19690332 ]
- Daub H, Olsen JV, Bairlein M, Gnad F, Oppermann FS, Korner R, Greff Z, Keri G, Stemmann O, Mann M: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell. 2008 Aug 8;31(3):438-48. doi: 10.1016/j.molcel.2008.07.007. [PubMed:18691976 ]
- Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, Cohn MA, Cantley LC, Gygi SP: Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12130-5. Epub 2004 Aug 9. [PubMed:15302935 ]
- Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. [PubMed:17081983 ]
- Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. [PubMed:19413330 ]
- Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science. 2007 May 25;316(5828):1160-6. [PubMed:17525332 ]
- Kuiperij HB, de Rooij J, Rehmann H, van Triest M, Wittinghofer A, Bos JL, Zwartkruis FJ: Characterisation of PDZ-GEFs, a family of guanine nucleotide exchange factors specific for Rap1 and Rap2. Biochim Biophys Acta. 2003 Feb 17;1593(2-3):141-9. [PubMed:12581858 ]
- Gao X, Satoh T, Liao Y, Song C, Hu CD, Kariya Ki K, Kataoka T: Identification and characterization of RA-GEF-2, a Rap guanine nucleotide exchange factor that serves as a downstream target of M-Ras. J Biol Chem. 2001 Nov 9;276(45):42219-25. Epub 2001 Aug 27. [PubMed:11524421 ]
|
