Hmdb loader
Identification
HMDB Protein ID HMDBP08444
Secondary Accession Numbers
  • 14156
Name Translation factor GUF1, mitochondrial
Synonyms
  1. Elongation factor 4 homolog
  2. GTPase GUF1
  3. Ribosomal back-translocase
  4. EF-4
Gene Name GUF1
Protein Type Unknown
Biological Properties
General Function Involved in GTP binding
Specific Function Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner (By similarity).
Pathways Not Available
Reactions
Guanosine triphosphate + Water → Guanosine diphosphate + Phosphate details
GO Classification
Biological Process
translation
GTP catabolic process
Cellular Component
mitochondrial inner membrane
Function
purine nucleotide binding
binding
nucleotide binding
catalytic activity
hydrolase activity
guanyl nucleotide binding
guanyl ribonucleotide binding
gtp binding
gtpase activity
nucleoside-triphosphatase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
pyrophosphatase activity
Molecular Function
GTPase activity
GTP binding
Cellular Location
  1. Cytoplasmic
Gene Properties
Chromosome Location 4
Locus 4p12
SNPs GUF1
Gene Sequence
>2010 bp
ATGTGGACCCTCGTGGGTCGGGGCTGGGGGTGCGCACGCGCTCTCGCGCCACGAGCCACT
GGGGCCGCGCTTCTGGTGGCCCCGGGGCCCCGGTCCGCGCCGACCCTTGGGGCTGCTCCA
GAGTCCTGGGCTACCGACAGGCTCTACAGCTCCGCAGAATTCAAGGAAAAACCTGACATG
TCTAGGTTTCCTGTTGAAAATATTAGAAATTTCAGTATTGTTGCACACGTGGATCATGGC
AAAAGTACTTTAACTGACAGGCTCCTAGAACTTACAGGGACAATTGATAAAACAAAGAAT
AATAAGCAGGTTCTTGATAAATTGCAAGTGGAACGAGAAAGAGGAATCACTGTTAAAGCA
CAGACAGCATCTCTCTTTTACAATTGTGAAGGAAAGCAGTACCTTTTAAATCTCATTGAT
ACACCGGGCCATGTTGATTTTAGTTATGAAGTATCCAGGTCACTTTCTGCTTGCCAGGGT
GTTTTACTTGTGGTTGATGCAAATGAGGGAATTCAAGCCCAAACTGTAGCAAACTTCTTT
CTTGCCTTCGAAGCACAGCTATCGGTAATTCCAGTTATAAATAAGATAGATCTGAAGAAT
GCTGATCCTGAAAGGGTTGAAAACCAAATTGAGAAAGTGTTTGATATTCCAAGTGATGAA
TGTATTAAGATTTCTGCTAAACTTGGAACAAATGTTGAGAGTGTTCTTCAGGCAATTATT
GAAAGAATCCCCCCTCCTAAAGTGCATCGCAAAAATCCTCTGAGAGCTTTGGTATTTGAC
TCCACCTTTGACCAGTATAGAGGTGTGATAGCCAATGTAGCATTATTTGACGGAGTGGTT
TCCAAAGGAGATAAAATTGTATCTGCACATACTCAAAAGACATACGAAGTTAATGAAGTA
GGAGTCTTGAATCCTAATGAGCAGCCAACTCATAAATTATATGCAGGACAGGTGGGCTAT
CTGATTGCTGGGATGAAAGATGTCACTGAAGCGCAAATAGGAGATACATTATGTTTACAT
AAGCAACCAGTGGAGCCCTTGCCTGGGTTTAAATCAGCGAAACCAATGGTATTTGCAGGA
ATGTATCCTCTAGACCAATCTGAATATAACAATCTGAAGAGTGCTATAGAAAAACTGACT
TTAAATGATTCCAGTGTGACCGTTCATCGGGATAGTAGCCTTGCTCTGGGTGCTGGCTGG
AGGCTAGGATTTCTTGGACTTTTGCACATGGAAGTTTTCAACCAGCGACCGGAGCAAGAA
TATAATGCTTCTGTTATTTTAACAACCCCTACTGTTCCATATAAAGCTGTACTGTCATCA
TCAAAATTGATAAAGGAACATAGAGAAAAAGAAATTACAATTATCAATCCTGCACAATTC
CCCGATAAATCAAAAGTAACAGAATATTTGGAGCCAGTTGTTTTGGGCACTATTATCACA
CCAGATGAATACACTGGAAAAATAATGATGCTTTGCGAGGCTCGAAGAGCAGTTCAGAAG
AATATGATATTTATTGATCAAAATAGAGTTATGCTTAAATATCTCTTTCCTTTGAATGAA
ATTGTGGTAGATTTTTATGACTCTTTGAAATCCCTATCTTCTGGATATGCTAGTTTTGAT
TACGAAGATGCAGGCTACCAGACTGCAGAACTTGTAAAAATGGATATTCTACTGAATGGA
AATACTGTAGAGGAGCTAGTAACTGTTGTACACAAAGACAAAGCTCATTCAATTGGCAAA
GCCATATGTGAACGGCTGAAGGATTCTCTTCCTAGGCAACTGTTTGAGATAGCAATTCAA
GCTGCTATTGGAAGTAAAATCATTGCGAGAGAAACAGTGAAAGCCTATAGGAAAAACGTT
TTGGCAAAATGTTGTGGTGGTGATATTACCCGAAAAATGAAGCTTTTGAAGAGACAAGCA
GAAGGGAAAAAAAAGCTGAGGAAAATTGGCAACGTTGAAGTTCCAAAAGATGCTTTCATA
AAAGTTCTGAAAACACAATCTTCTAAATAA
Protein Properties
Number of Residues 669
Molecular Weight 74327.2
Theoretical pI 8.59
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>GTP-binding protein GUF1 homolog
MWTLVGRGWGCARALAPRATGAALLVAPGPRSAPTLGAAPESWATDRLYSSAEFKEKLDM
SRFPVENIRNFSIVAHVDHGKSTLADRLLELTGTIDKTKNNKQVLDKLQVERERGITVKA
QTASLFYNCEGKQYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVANFF
LAFEAQLSVIPVINKIDLKNADPERVENQIEKVFDIPSDECIKISAKLGTNVESVLQAII
ERIPPPKVHRKNPLRALVFDSTFDQYRGVIANVALFDGVVSKGDKIVSAHTQKTYEVNEV
GVLNPNEQPTHKLYAGQVGYLIAGMKDVTEAQIGDTLCLHKQPVEPLPGFKSAKPMVFAG
MYPLDQSEYNNLKSAIEKLTLNDSSVTVHRDSSLALGAGWRLGFLGLLHMEVFNQRLEQE
YNASVILTTPTVPYKAVLSSSKLIKEHREKEITIINPAQFPDKSKVTEYLEPVVLGTIIT
PDEYTGKIMMLCEARRAVQKNMIFIDQNRVMLKYLFPLNEIVVDFYDSLKSLSSGYASFD
YEDAGYQTAELVKMDILLNGNTVEELVTVVHKDKAHSIGKAICERLKDSLPRQLFEIAIQ
AAIGSKIIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKLRKIGNVEVPKDAFI
KVLKTQSSK
GenBank ID Protein 10435150
UniProtKB/Swiss-Prot ID Q8N442
UniProtKB/Swiss-Prot Entry Name GUF1_HUMAN
PDB IDs Not Available
GenBank Gene ID AK023282
GeneCard ID GUF1
GenAtlas ID GUF1
HGNC ID HGNC:25799
References
General References
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  3. Vasilescu J, Zweitzig DR, Denis NJ, Smith JC, Ethier M, Haines DS, Figeys D: The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells. J Proteome Res. 2007 Jan;6(1):298-305. [PubMed:17203973 ]