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Showing Protein Mitogen-activated protein kinase kinase kinase MLT (HMDBP08759)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 3 metabolites
Identification
HMDB Protein ID HMDBP08759
Secondary Accession Numbers
  • 14482
Name Mitogen-activated protein kinase kinase kinase MLT
Synonyms
  1. HCCS-4
  2. Human cervical cancer suppressor gene 4 protein
  3. Leucine zipper- and sterile alpha motif-containing kinase
  4. MLK-like mitogen-activated protein triple kinase
  5. MLK-related kinase
  6. MRK
  7. Mixed lineage kinase-related kinase
  8. Sterile alpha motif- and leucine zipper-containing kinase AZK
Gene Name MLTK
Protein Type Unknown
Biological Properties
General Function Involved in protein kinase activity
Specific Function Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro- apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'
Pathways Not Available
Reactions Not Available
GO Classification
Function
binding
catalytic activity
transferase activity
transferase activity, transferring phosphorus-containing groups
kinase activity
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
atp binding
protein kinase activity
protein serine/threonine kinase activity
Process
phosphorus metabolic process
phosphate metabolic process
metabolic process
cellular metabolic process
protein amino acid phosphorylation
phosphorylation
Cellular Location
  1. Nucleus
  2. Cytoplasm
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs MLTK
Gene Sequence 
>2403 bp
ATGTCGTCTCTCGGTGCCTCCTTTGTGCAAATTAAATTTGATGACTTGCAGTTTTTTGAA
AACTGCGGTGGAGGAAGTTTTGGGAGTGTTTATCGAGCCAAATGGATATCACAGGACAAG
GAGGTGGCTGTAAAGAAGCTCCTCAAAATAGAGAAAGAGGCAGAAATACTCAGTGTCCTC
AGTCACAGAAACATCATCCAGTTTTATGGAGTAATTCTTGAACCTCCCAACTATGGCATT
GTCACAGAATATGCTTCTCTGGGATCACTCTATGATTACATTAACAGTAACAGAAGTGAG
GAGATGGATATGGATCACATTATGACCTGGGCCACTGATGTAGCCAAAGGAATGCATTAT
TTACATATGGAGGCTCCTGTCAAGGTGATTCACAGAGACCTCAAGTCAAGAAACGTTGTT
ATAGCTGCTGATGGAGTATTGAAGATCTGTGACTTTGGTGCCTCTCGGTTCCATAACCAT
ACAACACACATGTCCTTGGTTGGAACTTTCCCATGGATGGCTCCAGAAGTTATCCAGAGT
CTCCCTGTGTCAGAAACTTGTGACACATATTCCTATGGTGTGGTTCTCTGGGAGATGCTA
ACAAGGGAGGTCCCCTTTAAAGGTTTGGAAGGATTACAAGTAGCTTGGCTTGTAGTGGAA
AAAAACGAGAGATTAACCATTCCAAGCAGTTGCCCCAGAAGTTTTGCTGAACTGTTACAT
CAGTGTTGGGAAGCTGATGCCAAGAAACGGCCATCATTCAAGCAAATCATTTCAATCCTG
GAGTCCATGTCAAATGACACGAGCCTTCCTGACAAGTGTAACTCATTCCTACACAACAAG
GCGGAGTGGAGGTGCGAAATTGAGGCAACTCTTGAGAGGCTAAAGAAACTAGAGCGTGAT
CTCAGCTTTAAGGAGCAGGAGCTTAAAGAACGAGAAAGACGTTTAAAGATGTGGGAGCAA
AAGCTGACAGAGCAGTCCAACACCCCGCTGCTGCCTTCCTTTGAGATTGGTGCATGGACG
GAAGACGATGTGTATTGTTGGGTTCAGCAGCTCGTCAGAAAAGGTGACTCTTCAGCAGAG
ATGAGTGTATATGCAAGCTTGTTTAAAGAAAACAACATTACAGGGAAGCGGCTGCTGCTG
CTGGAGGAAGAAGACCTGAAAGACATGGGCATTGTCTCCAAGGGGCATATCATTCACTTC
AAGTCAGCCATTGAGAAATTAACCCATGATTACATAAATTTGTTTCACTTCCCACCACTA
ATTAAGGACTCAGGAGGTGAACCTGAAGAAAATGAGGAAAAAATAGTGAACCTGGAACTG
GTTTTTGGTTTTCACTTGAAACCAGGAACTGGCCCACAGGATTGTAAGTGGAAAATGTAT
ATGGAGATGGATGGGGATGAAATTGCAATAACCTACATAAAAGATGTGACATTCAACACT
AACCTACCTGATGCGGAGATTTTAAAGATGACAAAGCCACCATTTGTAATGGAGAAGTGG
ATTGTAGGAATAGCAAAAAGTCAGACTGTGGAGTGCACTGTCACATATGAGAGTGATGTT
AGAACTCCAAAAAGCACTAAACATGTCCATTCGATTCAGTGGAGTAGAACAAAACCTCAG
GATGAAGTGAAAGCAGTCCAACTTGCCATTCAGACATTATTCACCAATTCAGATGGCAAC
CCTGGAAGCAGGTCCGACTCAAGTGCTGATTGCCAGTGGTTAGATACTCTGAGGATGCGG
CAGATTGCATCCAACACTTCTTTACAGCGTTCCCAGAGCAATCCTATTCTGGGGTCACCG
TTCTTCTCACACTTTGATGGCCAGGATTCCTACGCTGCTGCTGTGAGACGGCCCCAGGTG
CCCATTAAGTATCAACAGATTACACCTGTGAACCAGTCCAGAAGCTCGTCTCCTACTCAG
TATGGACTGACCAAAAACTTCTCTTCCCTACATCTCAACTCTAGGGACAGTGGCTTTTCC
AGTGGCAATACTGACACCTCTTCAGAGAGGGGTCGATACTCAGACAGAAGCAGGAACAAA
TATGGACGTGGTAGTATATCACTCAATTCTTCTCCTAGAGGAAGATACAGTGGAAAGAGT
CAGCATTCCACTCCTTCAAGAGGAAGATACCCTGGAAAGTTCTACAGGGTTTCTCAGTCA
GCACTCAATCCTCACCAGTCGCCTGACTTCAAGAGAAGCCCCAGGGACCTCCACCAACCC
AACACCATACCAGGGATGCCTTTGCACCCTGAGACTGACTCAAGAGCCAGTGAAGAGGAC
AGCAAAGTCAGCGAAGGGGGCTGGACAAAAGTGGAATACCGGAAAAAGCCCCACAGGCCA
TCTCCCGCCAAAACCAATAAAGAGAGAGCCAGAGGGGACCACCGTGGATGGAGAAACTTT
TGA
Protein Properties
Number of Residues 800
Molecular Weight 91180.4
Theoretical pI 7.95
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence 
>Mitogen-activated protein kinase kinase kinase MLT
MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVL
SHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHY
LHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQS
LPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLH
QCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERD
LSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQLVRKGDSSAE
MSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPL
IKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNT
NLPDAEILKMTKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHLIQWSRTKPQ
DEVKAVQLAIQTLFTNSDGNPGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSP
FFSHFDGQDSYAAAVRRPQVPIKYQQITPVNQSRSSSPTQYGLTKNFSSLHLNSRDSGFS
SGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKSQHSTPSRGRYPGKFYRVSQS
ALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTKVEYRKKPHRP
SPAKTNKERARGDHRGWRNF
GenBank ID Protein 9927293
UniProtKB/Swiss-Prot ID Q9NYL2
UniProtKB/Swiss-Prot Entry Name MLTK_HUMAN
PDB IDs Not Available
GenBank Gene ID AB030034
GeneCard ID MLTK
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
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  4. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [PubMed:18669648 ]
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  6. Daub H, Olsen JV, Bairlein M, Gnad F, Oppermann FS, Korner R, Greff Z, Keri G, Stemmann O, Mann M: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell. 2008 Aug 8;31(3):438-48. doi: 10.1016/j.molcel.2008.07.007. [PubMed:18691976 ]
  7. Oppermann FS, Gnad F, Olsen JV, Hornberger R, Greff Z, Keri G, Mann M, Daub H: Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics. 2009 Jul;8(7):1751-64. doi: 10.1074/mcp.M800588-MCP200. Epub 2009 Apr 15. [PubMed:19369195 ]
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  9. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. [PubMed:19413330 ]
  10. Beausoleil SA, Villen J, Gerber SA, Rush J, Gygi SP: A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol. 2006 Oct;24(10):1285-92. Epub 2006 Sep 10. [PubMed:16964243 ]
  11. Cantin GT, Yi W, Lu B, Park SK, Xu T, Lee JD, Yates JR 3rd: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res. 2008 Mar;7(3):1346-51. doi: 10.1021/pr0705441. Epub 2008 Jan 26. [PubMed:18220336 ]
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  14. Tosti E, Waldbaum L, Warshaw G, Gross EA, Ruggieri R: The stress kinase MRK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. J Biol Chem. 2004 Nov 12;279(46):47652-60. Epub 2004 Sep 1. [PubMed:15342622 ]
  15. Liu TC, Huang CJ, Chu YC, Wei CC, Chou CC, Chou MY, Chou CK, Yang JJ: Cloning and expression of ZAK, a mixed lineage kinase-like protein containing a leucine-zipper and a sterile-alpha motif. Biochem Biophys Res Commun. 2000 Aug 11;274(3):811-6. [PubMed:10924358 ]
  16. Gotoh I, Adachi M, Nishida E: Identification and characterization of a novel MAP kinase kinase kinase, MLTK. J Biol Chem. 2001 Feb 9;276(6):4276-86. Epub 2000 Oct 19. [PubMed:11042189 ]
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  19. Yang JJ: A novel zinc finger protein, ZZaPK, interacts with ZAK and stimulates the ZAK-expressing cells re-entering the cell cycle. Biochem Biophys Res Commun. 2003 Jan 31;301(1):71-7. [PubMed:12535642 ]
  20. Takahashi M, Gotoh Y, Isagawa T, Nishimura T, Goyama E, Kim HS, Mukai H, Ono Y: Regulation of a mitogen-activated protein kinase kinase kinase, MLTK by PKN. J Biochem. 2003 Feb;133(2):181-7. [PubMed:12761180 ]
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  22. Choi HS, Choi BY, Cho YY, Zhu F, Bode AM, Dong Z: Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha. J Biol Chem. 2005 Apr 8;280(14):13545-53. Epub 2005 Jan 31. [PubMed:15684425 ]