Hmdb loader
Identification
HMDB Protein ID HMDBP09284
Secondary Accession Numbers
  • 15112
Name CCR4-NOT transcription complex subunit 4
Synonyms
  1. CCR4-associated factor 4
  2. E3 ubiquitin-protein ligase CNOT4
  3. Potential transcriptional repressor NOT4Hp
Gene Name CNOT4
Protein Type Enzyme
Biological Properties
General Function Involved in nucleotide binding
Specific Function Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex
Pathways Not Available
Reactions Not Available
GO Classification
Function
ion binding
cation binding
metal ion binding
binding
nucleotide binding
transition metal ion binding
zinc ion binding
protein binding
nucleic acid binding
Cellular Location
  1. Cytoplasm (Probable)
  2. Nucleus (Probable)
Gene Properties
Chromosome Location Chromosome:7
Locus 7q22-qter
SNPs CNOT4
Gene Sequence
>1728 bp
ATGTCTCGCAGTCCTGATGCGAAGGAAGACCCTGTGGAGTGCCCTCTTTGCATGGAGCCC
TTGGAGATAGATGATATCAACTTTTTCCCTTGCACCTGTGGCTACCAGATTTGCCGATTT
TGTTGGCATCGAATTCGCACTGATGAAAATGGGCTTTGTCCTGCATGTAGAAAGCCATAT
CCAGAAGACCCAGCAGTTTATAAACCACTCTCCCAGGAAGAGCTGCAAAGGATAAAGAAT
GAGAAAAAACAGAAACAAAATGAGAGAAAACAGAAAATATCAGAAAATCGCAAACATTTG
GCTAGTGTACGTGTCGTACAAAAAAACCTCGTCTTTGTTGTAGGTTTATCTCAGCGCCTA
GCAGACCCAGAGGTTTTAAAACGACCAGAATATTTTGGGAAGTTTGGTAAAATACATAAA
GTTGTCATCAATAATAGCACATCATATGCAGGCTCACAGGGTCCAAGTGCCAGTGCTTAT
GTAACCTATATCCGGTCAGAAGACGCTCTCAGAGCCATACAGTGTGTCAACAATGTGGTA
GTAGATGGCAGAACACTTAAGGCATCTCTAGGTACAACAAAATACTGCAGTTACTTCTTA
AAGAATATGCAGTGTCCAAAACCTGACTGCATGTATCTTCATGAATTGGGGGATGAGGCG
GCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAACACCAAGAATATGAACAGAAGCTA
CTTCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGGTTCAGTTGAT
AAAAATAAGAACAAAGTGACACCACTGCAGAGGTACGATACCCCCATTGACAAACCTTCA
GATTCTCTCAGTATAGGGAACGGTGATAATTCCCAGCAGATATCTAACAGTGATACGCCT
TCACCACCACCTGGTTTGTCAAAATCCAATCCAGTCATCCCCATCAGTTCATCCAATCAC
AGTGCACGGTCCCCTTTTGAAGGGGCAGTAACAGAGTCACAGTCGTTATTCTCAGACAAT
TTTCGCCATCCCAACCCTATCCCAAGTGGGCTTCCTCCTTTCCCCAGCTCCCCACAGACA
TCCAGTGACTGGCCTACAGCACCAGAACCACAGAGCCTCTTCACATCAGAAACAATCCCA
GTATCATCCTCTACAGACTGGCAAGCAGCTTTTGGCTTTGGTTCTTCTAAACAACCAGAG
GATGACTTGGGTTTTGATCCCTTCGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAG
AAGGAACTGTCCGTTCAAGACCAACCTTCCCTTTCGCCCACATCTCTTCAGAACTCCTCT
TCACACACTACAACCGCCAAAGGTCCAGGCTCTGGATTCCTGCATCCTGCTGCAGCTACA
AATGCCAATTCTCTCAATAGTACCTTTTCAGTCTTGCCCCAGAGGTTCCCTCAATTTCAG
CAGCACCGAGCGGTTTATAATTCATTCAGTTTTCCAGGCCAGGCAGCCCGCTATCCTTGG
ATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACACAGCAAACCCCACCTCAAAT
AGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCCT
GTAGCAGGGGAAGAAGAGGTGAAGGTTTCGACCATGCCACTGTCAACCTCTTCCCATTCA
TTACAACAAGGACAGCAGCCTACAAGTCTCCACACTACTGTGGCCTGA
Protein Properties
Number of Residues 575
Molecular Weight 63509.5
Theoretical pI 7.04
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence
>CCR4-NOT transcription complex subunit 4
MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPY
PEDPAVYKPLSQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRL
ADPEVLKRPEYFGKFGKIHKVVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVV
VDGRTLKASLGTTKYCSYFLKNMQCPKPDCMYLHELGDEAASFTKEEMQAGKHQEYEQKL
LQELYKLNPNFLQLSTGSVDKNKNKVTPLQRYDTPIDKPSDSLSIGNGDNSQQISNSDTP
SPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPPFPSSPQT
SSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIE
KELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQ
QHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIP
VAGEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA
GenBank ID Protein 300069013
UniProtKB/Swiss-Prot ID O95628
UniProtKB/Swiss-Prot Entry Name CNOT4_HUMAN
PDB IDs
GenBank Gene ID NM_001190848.1
GeneCard ID CNOT4
GenAtlas ID CNOT4
HGNC ID HGNC:7880
References
General References
  1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [PubMed:14702039 ]
  2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  3. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [PubMed:18669648 ]
  4. Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. [PubMed:19690332 ]
  5. Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science. 2007 May 25;316(5828):1160-6. [PubMed:17525332 ]
  6. Hillier LW, Fulton RS, Fulton LA, Graves TA, Pepin KH, Wagner-McPherson C, Layman D, Maas J, Jaeger S, Walker R, Wylie K, Sekhon M, Becker MC, O'Laughlin MD, Schaller ME, Fewell GA, Delehaunty KD, Miner TL, Nash WE, Cordes M, Du H, Sun H, Edwards J, Bradshaw-Cordum H, Ali J, Andrews S, Isak A, Vanbrunt A, Nguyen C, Du F, Lamar B, Courtney L, Kalicki J, Ozersky P, Bielicki L, Scott K, Holmes A, Harkins R, Harris A, Strong CM, Hou S, Tomlinson C, Dauphin-Kohlberg S, Kozlowicz-Reilly A, Leonard S, Rohlfing T, Rock SM, Tin-Wollam AM, Abbott A, Minx P, Maupin R, Strowmatt C, Latreille P, Miller N, Johnson D, Murray J, Woessner JP, Wendl MC, Yang SP, Schultz BR, Wallis JW, Spieth J, Bieri TA, Nelson JO, Berkowicz N, Wohldmann PE, Cook LL, Hickenbotham MT, Eldred J, Williams D, Bedell JA, Mardis ER, Clifton SW, Chissoe SL, Marra MA, Raymond C, Haugen E, Gillett W, Zhou Y, James R, Phelps K, Iadanoto S, Bubb K, Simms E, Levy R, Clendenning J, Kaul R, Kent WJ, Furey TS, Baertsch RA, Brent MR, Keibler E, Flicek P, Bork P, Suyama M, Bailey JA, Portnoy ME, Torrents D, Chinwalla AT, Gish WR, Eddy SR, McPherson JD, Olson MV, Eichler EE, Green ED, Waterston RH, Wilson RK: The DNA sequence of human chromosome 7. Nature. 2003 Jul 10;424(6945):157-64. [PubMed:12853948 ]
  7. Winkler GS, Albert TK, Dominguez C, Legtenberg YI, Boelens R, Timmers HT: An altered-specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair. J Mol Biol. 2004 Mar 12;337(1):157-65. [PubMed:15001359 ]
  8. Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT: Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. Nucleic Acids Res. 2000 Feb 1;28(3):809-17. [PubMed:10637334 ]
  9. Albert TK, Hanzawa H, Legtenberg YI, de Ruwe MJ, van den Heuvel FA, Collart MA, Boelens R, Timmers HT: Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J. 2002 Feb 1;21(3):355-64. [PubMed:11823428 ]
  10. Hanzawa H, de Ruwe MJ, Albert TK, van Der Vliet PC, Timmers HT, Boelens R: The structure of the C4C4 ring finger of human NOT4 reveals features distinct from those of C3HC4 RING fingers. J Biol Chem. 2001 Mar 30;276(13):10185-90. Epub 2000 Nov 21. [PubMed:11087754 ]