Hmdb loader
Identification
HMDB Protein ID HMDBP09292
Secondary Accession Numbers
  • 15122
Name Ubiquitin-like modifier-activating enzyme 6
Synonyms
  1. E1-L2
  2. MOP-4
  3. Monocyte protein 4
  4. Ubiquitin-activating enzyme 6
  5. Ubiquitin-activating enzyme E1-like protein 2
Gene Name UBA6
Protein Type Enzyme
Biological Properties
General Function Involved in catalytic activity
Specific Function Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility
Pathways Not Available
Reactions Not Available
GO Classification
Function
binding
catalytic activity
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
atp binding
small protein activating enzyme activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
Cellular Location Not Available
Gene Properties
Chromosome Location Chromosome:4
Locus 4q13.2
SNPs UBA6
Gene Sequence
>3159 bp
ATGGAAGGATCCGAGCCTGTGGCCGCCCATCAGGGGGAAGAGGCGTCCTGTTCTTCCTGG
GGGACTGGCAGCACAAATAAAAATTTGCCCATTATGTCAACAGCATCTGTGGAAATCGAT
GATGCATTGTATAGTCGACAGAGGTACGTTCTTGGAGACACAGCAATGCAGAAGATGGCC
AAGTCCCATGTTTTCTTAAGTGGGATGGGTGGTCTTGGTTTGGAAATTGCAAAGAATCTT
GTTCTTGCAGGGATTAAGGCAGTTACAATTCATGATACAGAAAAATGCCAAGCATGGGAT
CTAGGAACCAACTTCTTTCTCAGTGAAGATGATGTTGTTAATAAGAGAAACAGGGCTGAA
GCTGTACTTAAACATATTGCAGAACTAAATCCATACGTTCATGTCACATCATCTTCTGTT
CCTTTCAATGAGACCACAGATCTCTCCTTTTTAGATAAATACCAGTGTGTAGTATTGACT
GAGATGAAACTTCCATTGCAGAAGAAGATCAATGACTTTTGCCGTTCTCAGTGCCCTCCA
ATTAAGTTTATCAGTGCAGATGTACATGGAATTTGGTCAAGGTTATTTTGTGATTTCGGT
GATGAATTTGAAGTTTTAGATACAACAGGAGAAGAACCAAAAGAAATTTTCATTTCAAAC
ATAACGCAAGCAAATCCTGGCATTGTTACTTGCCTTGAAAATCATCCTCACAAACTGGAG
ACAGGACAATTCCTAACATTTCGAGAAATTAATGGAATGACAGGTTTAAATGGATCTATA
CAACAAATAACGGTGATATCGCCATTTTCTTTTAGTATTGGTGACACCACAGAACTGGAA
CCATATTTACATGGAGGCATAGCTGTCCAAGTTAAGACTCCTAAAACAGTTTTTTTTGAA
TCACTGGAGAGGCAGTTAAAACATCCAAAGTGCCTTATTGTGGATTTTAGCAACCCTGAG
GCACCTTTAGAGATTCACACAGCTATGCTTGCCTTGGACCAGTTTCAGGAGAAATACAGT
CGCAAGCCAAATGTTGGATGCCAACAAGATTCAGAAGAACTGTTGAAACTAGCAACATCT
ATAAGTGAAACCTTGGAAGAGAAGCCTGATGTAAATGCTGACATTGTGCATTGGCTCTCT
TGGACTGCCCAAGGCTTTTTATCTCCACTTGCTGCAGCAGTAGGAGGTGTTGCCAGCCAA
GAAGTATTGAAAGCTGTAACAGGAAAATTTTCTCCTTTGTGCCAGTGGTTATATCTTGAA
GCAGCAGATATTGTTGAATCACTAGGCAAACCTGAATGTGAAGAATTTCTCCCACGAGGA
GATAGATATGATGCCTTAAGAGCTTGCATTGGAGACACTTTGTGTCAGAAACTGCAAAAT
TTAAACATCTTCTTAGTAGGGTGTGGAGCCATAGGCTGTGAAATGTTGAAAAATTTTGCT
TTACTTGGTGTTGGCACAAGCAAAGAGAAAGGAATGATTACAGTTACAGATCCTGACTTG
ATAGAGAAATCCAACTTAAATAGACAGTTCCTATTTCGTCCTCATCACATACAGAAACCT
AAAAGCTACACTGCTGCTGATGCTACTCTGAAAATAAATTCTCAAATAAAGATAGATGCA
CACCTGAACAAAGTATGTCCAACCACTGAGACCATTTACAATGATGAGTTCTATACTAAA
CAAGATGTAATTATTACAGCATTAGATAATGTGGAAGCCAGGAGATACGTAGACAGTCGT
TGCTTAGCAAATCTAAGGCCTCTTTTAGATTCTGGAACAATGGGCACTAAGGGACACACT
GAAGTTATTGTACCGCATTTGACTGAGTCTTACAATAGTCATCGGGATCCCCCAGAAGAG
GAAATACCATTTTGTACTCTAAAATCCTTTCCAGCTGCTATTGAACATACCATACAGTGG
GCAAGAGATAAGTTTGAAAGTTCCTTTTCCCACAAACCTTCATTGTTTAACAAATTTTGG
CAAACCTATTCATCTGCAGAAGAAGTCTTACAGAAGATACAGAGTGGACACAGTTTAGAA
GGCTGTTTTCAAGTTATAAAGTTACTTAGCAGAAGACCTAGAAATTGGTCCCAGTGTGTA
GAATTAGCAAGATTAAAGTTTGAAAAATATTTTAACCATAAGGCTCTTCAGCTTCTTCAC
TGTTTCCCTCTGGACATACGATTAAAAGATGGCAGTTTATTTTGGCAGTCACCAAAGAGG
CCACCCTCTCCAATAAAATTTGATTTAAATGAGCCTTTGCACCTCAGTTTCCTTCAGAAT
GCTGCAAAACTATATGCTACAGTATATTGTATTCCATTTGCAGAAGAGGACTTATCAGCA
GATGCCCTCTTGAATATTCTTTCAGAAGTAAAGATTCAGGAATTCAAGCCTTCCAATAAG
GTTGTTCAAACAGATGAAACTGCAAGGAAACCAGACCATGTTCCTATTAGCAGTGAAGAT
GAGAGGAATGCAATTTTCCAACTAGAAAAGGCTATTTTATCTAATGAAGCCACCAAAAGT
GACCTTCAGATGGCAGTGCTTTCATTTGAAAAAGATGATGATCATAATGGACACATAGAT
TTCATCACAGCTGCATCAAATCTTCGTGCCAAAATGTACAGCATTGAACCAGCTGACCGT
TTCAAAACAAAGCGCATAGCTGGTAAAATTATACCTGCTATAGCAACAACCACTGCTACA
GTTTCTGGCTTGGTTGCCTTGGAGATGATCAAAGTAACTGGTGGCTATCCATTTGAAGTT
TACAAAAATTGTTTTCTTAACTTAGCCATTCCAATTGTAGTATTTACAGAGACAACTGAA
GTAAGGAAAACTAAAATCAGAAATGGAATATCATTTACAATTTGGGATCGATGGACCGTA
CATGGAAAAGAAGATTTCACCCTCTTGGATTTCATAAATGCAGTCAAAGAGAAGTATGGA
ATTGAGCCAACAATGGTGGTACAGGGAGTCAAAATGCTTTATGTTCCTGTAATGCCTGGT
CATGCAAAAAGATTGAAGTTAACAATGCATAAACTTGTAAAACCTACTACTGAAAAGAAA
TATGTGGATCTTACTGTGTCATTTGCTCCAGACATTGATGGAGATGAAGATTTGCCGGGA
CCTCCAGTAAGATACTACTTCAGTCATGACACTGATTAA
Protein Properties
Number of Residues 1052
Molecular Weight 117968.9
Theoretical pI 6.05
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence
>Ubiquitin-like modifier-activating enzyme 6
MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMA
KSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAE
AVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPP
IKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLE
TGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFE
SLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATS
ISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLE
AADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFA
LLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDA
HLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHT
EVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFW
QTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLH
CFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEEDLSA
DALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKS
DLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTAT
VSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTV
HGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKK
YVDLTVSFAPDIDGDEDLPGPPVRYYFSHDTD
GenBank ID Protein 11990422
UniProtKB/Swiss-Prot ID A0AVT1
UniProtKB/Swiss-Prot Entry Name UBA6_HUMAN
PDB IDs Not Available
GenBank Gene ID AB014773
GeneCard ID UBA6
GenAtlas ID UBA6
HGNC ID HGNC:25581
References
General References
  1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [PubMed:14702039 ]
  2. Hillier LW, Graves TA, Fulton RS, Fulton LA, Pepin KH, Minx P, Wagner-McPherson C, Layman D, Wylie K, Sekhon M, Becker MC, Fewell GA, Delehaunty KD, Miner TL, Nash WE, Kremitzki C, Oddy L, Du H, Sun H, Bradshaw-Cordum H, Ali J, Carter J, Cordes M, Harris A, Isak A, van Brunt A, Nguyen C, Du F, Courtney L, Kalicki J, Ozersky P, Abbott S, Armstrong J, Belter EA, Caruso L, Cedroni M, Cotton M, Davidson T, Desai A, Elliott G, Erb T, Fronick C, Gaige T, Haakenson W, Haglund K, Holmes A, Harkins R, Kim K, Kruchowski SS, Strong CM, Grewal N, Goyea E, Hou S, Levy A, Martinka S, Mead K, McLellan MD, Meyer R, Randall-Maher J, Tomlinson C, Dauphin-Kohlberg S, Kozlowicz-Reilly A, Shah N, Swearengen-Shahid S, Snider J, Strong JT, Thompson J, Yoakum M, Leonard S, Pearman C, Trani L, Radionenko M, Waligorski JE, Wang C, Rock SM, Tin-Wollam AM, Maupin R, Latreille P, Wendl MC, Yang SP, Pohl C, Wallis JW, Spieth J, Bieri TA, Berkowicz N, Nelson JO, Osborne J, Ding L, Meyer R, Sabo A, Shotland Y, Sinha P, Wohldmann PE, Cook LL, Hickenbotham MT, Eldred J, Williams D, Jones TA, She X, Ciccarelli FD, Izaurralde E, Taylor J, Schmutz J, Myers RM, Cox DR, Huang X, McPherson JD, Mardis ER, Clifton SW, Warren WC, Chinwalla AT, Eddy SR, Marra MA, Ovcharenko I, Furey TS, Miller W, Eichler EE, Bork P, Suyama M, Torrents D, Waterston RH, Wilson RK: Generation and annotation of the DNA sequences of human chromosomes 2 and 4. Nature. 2005 Apr 7;434(7034):724-31. [PubMed:15815621 ]
  3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  4. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M: Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science. 2009 Aug 14;325(5942):834-40. doi: 10.1126/science.1175371. Epub 2009 Jul 16. [PubMed:19608861 ]
  5. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. [PubMed:19413330 ]
  6. Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. BMC Genomics. 2007 Oct 31;8:399. [PubMed:17974005 ]
  7. Zhu H, Zhou ZM, Huo R, Huang XY, Lu L, Lin M, Wang LR, Zhou YD, Li JM, Sha JH: Identification and characteristics of a novel E1 like gene nUBE1L in human testis. Acta Biochim Biophys Sin (Shanghai). 2004 Mar;36(3):227-34. [PubMed:15202508 ]
  8. Jin J, Li X, Gygi SP, Harper JW: Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature. 2007 Jun 28;447(7148):1135-8. [PubMed:17597759 ]
  9. Chiu YH, Sun Q, Chen ZJ: E1-L2 activates both ubiquitin and FAT10. Mol Cell. 2007 Sep 21;27(6):1014-23. [PubMed:17889673 ]