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Identification
HMDB Protein ID HMDBP09373
Secondary Accession Numbers
  • 15237
Name Hyaluronidase PH-20
Synonyms
  1. SubName: Sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), isoform CRA_c
  2. Hyal-PH20
  3. Hyaluronoglucosaminidase PH-20
  4. Sperm adhesion molecule 1
  5. Sperm surface protein PH-20
Gene Name SPAM1
Protein Type Enzyme
Biological Properties
General Function Involved in catalytic activity
Specific Function Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Pathways
  • Glycosaminoglycan degradation
Reactions
+ Water → DWA-2 + N-Acetylgalactosamine details
+ Water → DWA-2 + N-Acetylgalactosamine 4-sulphate details
GO Classification
Biological Process
fusion of sperm to egg plasma membrane
cell adhesion
carbohydrate metabolic process
binding of sperm to zona pellucida
Cellular Component
anchored to membrane
plasma membrane
Function
hyalurononglucosaminidase activity
hexosaminidase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing o-glycosyl compounds
Molecular Function
hyalurononglucosaminidase activity
Process
cellular process involved in reproduction
cellular process involved in reproduction in multicellular organism
fusion of sperm to egg plasma membrane
metabolic process
primary metabolic process
carbohydrate metabolic process
cellular process
Cellular Location
  1. Cytoplasmic
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs SPAM1
Gene Sequence
>1536 bp
ATGGGAGTGCTAAAATTCAAGCACATCTTTTTCAGAAGCTTTGTTAAATCAAGTGGAGTA
TCCCAGATAGTTTTCACCTTCCTTCTGATTCCATGTTGCTTGACTCTGAATTTCAGAGCA
CCTCCTGTTATTCCAAATGTGCCTTTCCTCTGGGCCTGGAATGCCCCAAGTGAATTTTGT
CTTGGAAAATTTGATGAGCCACTAGATATGAGCCTCTTCTCTTTCATAGGAAGCCCCCGA
ATAAACGCCACCGGGCAAGGTGTTACAATATTTTATGTTGATAGACTTGGCTACTATCCT
TACATAGATTCAATCACAGGAGTAACTGTGAATGGAGGAATCCCCCAGAAGATTTCCTTA
CAAGACCATCTGGACAAAGCTAAGAAAGACATTACATTTTATATGCCAGTAGACAATTTG
GGAATGGCTGTTATTGACTGGGAAGAATGGAGACCCACTTGGGCAAGAAACTGGAAACCT
AAAGATGTTTACAAGAATAGGTCTATTGAATTGGTTCAGCAACAAAATGTACAACTTAGT
CTCACAGAGGCCACTGAGAAAGCAAAACAAGAATTTGAAAAAGCAGGGAAGGATTTCCTG
GTAGAGACTATAAAATTGGGAAAATTACTTCGGCCAAATCACTTGTGGGGTTATTATCTT
TTTCCGGATTGTTACAACCATCACTATAAGAAACCCGGTTACAATGGAAGTTGCTTCAAT
GTAGAAATAAAAAGAAATGATGATCTCAGCTGGTTGTGGAATGAAAGCACTGCTCTTTAC
CCATCCATTTATTTGAACACTCAGCAGTCTCCTGTAGCTGCTACACTCTATGTGCGCAAT
CGAGTTCGGGAAGCCATCAGAGTTTCCAAAATACCTGATGCAAAAAGTCCACTTCCGGTT
TTTGCATATACCCGCATAGTTTTTACTGATCAAGTTTTGAAATTCCTTTCTCAAGATGAA
CTTGTGTATACATTTGGCGAAACTGTTGCTCTGGGTGCTTCTGGAATTGTAATATGGGGA
ACCCTCAGTATAATGCGAAGTATGAAATCTTGCTTGCTCCTAGACAATTACATGGAGACT
ATACTGAATCCTTACATAATCAACGTCACACTAGCAGCCAAAATGTGTAGCCAAGTGCTT
TGCCAGGAGCAAGGAGTGTGTATAAGGAAAAACTGGAATTCAAGTGACTATCTTCACCTC
AACCCAGATAATTTTGCTATTCAACTTGAGAAAGGTGGAAAGTTCACAGTACGTGGAAAA
CCGACACTTGAAGACCTGGAGCAATTTTCTGAAAAATTTTATTGCAGCTGTTATAGCACC
TTGAGTTGTAAGGAGAAAGCTGATGTAAAAGACACTGATGCTGTTGATGTGTGTATTGCT
GATGGTGTCTGTATAGATGCTTTTCTAAAACCTCCCATGGAGACAGAAGAACCTCAAATT
TTCTACAATGCTTCACCCTCCACACTATCTGCCACAATGTTCATTTGGAGGCTGGAAGTC
TGGGATCAAGGTATTAGCAGAATTGGTTTCTTCTGA
Protein Properties
Number of Residues 511
Molecular Weight Not Available
Theoretical pI Not Available
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>Sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFC
LGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISL
QDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLS
LTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFN
VEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPV
FAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMET
ILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGK
PTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQI
FYNASPSTLSATMFIWRLEVWDQGISRIGFF
GenBank ID Protein 20072878
UniProtKB/Swiss-Prot ID P38567
UniProtKB/Swiss-Prot Entry Name Q8TC30_HUMAN
PDB IDs Not Available
GenBank Gene ID BC026163
GeneCard ID SPAM1
GenAtlas ID SPAM1
HGNC ID HGNC:11217
References
General References
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