Hmdb loader
Identification
HMDB Protein ID HMDBP11880
Secondary Accession Numbers None
Name Histone lysine demethylase PHF8
Synonyms
  1. PHD finger protein 8
Gene Name PHF8
Protein Type Unknown
Biological Properties
General Function Not Available
Specific Function Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
Pathways Not Available
Reactions
Protein N(6),N(6)-dimethyl-L-lysine + Oxoglutaric acid + Oxygen → protein N(6)-methyl-L-lysine + Succinic acid + Formaldehyde + CO(2) details
Protein N(6)-methyl-L-lysine + Oxoglutaric acid + Oxygen → protein L-lysine + Succinic acid + Formaldehyde + CO(2) details
GO Classification
Biological Process
negative regulation of chromatin silencing at rDNA
G1/S transition of mitotic cell cycle
brain development
transcription, DNA-dependent
positive regulation of transcription from RNA polymerase I promoter
Cellular Component
nucleolus
Molecular Function
histone demethylase activity (H3-K27 specific)
histone demethylase activity (H3-K9 specific)
histone demethylase activity (H4-K20 specific)
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
iron ion binding
methylated histone residue binding
zinc ion binding
chromatin binding
histone demethylase activity (H3-K36 specific)
Cellular Location Not Available
Gene Properties
Chromosome Location X
Locus Xp11.22
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues Not Available
Molecular Weight 117862.955
Theoretical pI 8.718
Pfam Domain Function Not Available
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>>gi|296531349|ref|NP_001171825.1| histone lysine demethylase PHF8 isoform 1 [Homo sapiens]
MNRSRAIVQRGRVLPPPAPLDTTNLAGRRTLQGRAKMASVPVYCLCRLPYDVTRFMIECD
MCQDWFHGSC
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID Q9UPP1
UniProtKB/Swiss-Prot Entry Name Not Available
PDB IDs
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID HGNC:20672
References
General References Not Available