| Identification |
|---|
| HMDB Protein ID
| HMDBP12316 |
| Secondary Accession Numbers
| None |
| Name
| Dioscorin DB3S |
| Synonyms
|
- Tuber storage protein DB3S
|
| Gene Name
| DB3S |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Storage protein of tuber. Involved in protection against oxidative stress (Probable). Has carbonate dehydratase and weak trypsin inhibitor activity detected by measuring the dehydration of sodium bicarbonate and the inhibition of trypsin-catalyzed hydrolysis of N-benzoyl-L-arginine-4-nitro anilide, respectively (PubMed:10552514). Contrarily, no carbonate dehydratase or trypsin inhibitor activity detected by measuring the hydrolysis of 4-nitrophenyl acetate or the inhibition of bovine trypsin-catalyzed hydrolysis of N-benzoyl-L-arginine ethyl ester, respectively (PubMed:15047697). Has dehydroascorbate (DHA) reductase and monodehydroascorbate (MDA) reductase activities (Ref.5). Catalyzes the reactions of carbonate dehydratase and DHA reductase independently of zinc and glutathione (GSH). The coupled reaction is capable of recycling a plant antioxidant ascorbate using ubiquitous compounds H(2)O and CO(2) (By similarity). Exhibits antioxidant activity. Able to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radicals (PubMed:11600050). Exhibits immunomodulatory activity. Activates Toll-like receptor 4 signaling pathways by up-regulating the gene expression of proinflammatory cytokines, such as tumor necrosis factor alpha, interleukin-1 beta and interleukin-6, and chemokines RANTES and MCP-1, in mouse RAW 264.7 macrophages. Stimulates the phagocytosis of E.coli by the LPS-treated mouse macrophages (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| response to oxygen radical |
| carbon utilization |
| cellular oxidant detoxification |
| cellular response to carbon dioxide |
| negative regulation of endopeptidase activity |
| positive regulation of phagocytosis |
| positive regulation of gene expression |
| Molecular Function |
| antioxidant activity |
| monodehydroascorbate reductase (NADH) activity |
| L-ascorbic acid binding |
| nutrient reservoir activity |
| serine-type endopeptidase inhibitor activity |
| zinc ion binding |
| protein homodimerization activity |
| carbonate dehydratase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 30460.195 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| Q75N34 |
| UniProtKB/Swiss-Prot Entry Name
| DB3S_DIOPO |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Gaidamashvili M, Ohizumi Y, Iijima S, Takayama T, Ogawa T, Muramoto K: Characterization of the yam tuber storage proteins from Dioscorea batatas exhibiting unique lectin activities. J Biol Chem. 2004 Jun 18;279(25):26028-35. doi: 10.1074/jbc.M402139200. Epub 2004 Mar 26. [PubMed:15047697 ]
- Hur GY, Park HJ, Kim HA, Ye YM, Park HS: Identification of Dioscorea batatas (sanyak) allergen as an inhalant and oral allergen. J Korean Med Sci. 2008 Feb;23(1):72-6. doi: 10.3346/jkms.2008.23.1.72. [PubMed:18303202 ]
- Hou WC, Liu JS, Chen HJ, Chen TE, Chang CF, Lin YH: Dioscorin, the major tuber storage protein of yam (Dioscorea batatas decne) with carbonic anhydrase and trypsin inhibitor activities. J Agric Food Chem. 1999 May;47(5):2168-72. doi: 10.1021/jf980738o. [PubMed:10552514 ]
- Xu YY, Yin J: Identification of a thermal stable allergen in yam (Dioscorea opposita) to cause anaphylaxis. Asia Pac Allergy. 2018 Jan 12;8(1):e4. doi: 10.5415/apallergy.2018.8.e4. eCollection 2018 Jan. [PubMed:29423371 ]
- Hou WC, Lee MH, Chen HJ, Liang WL, Han CH, Liu YW, Lin YH: Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J Agric Food Chem. 2001 Oct;49(10):4956-60. doi: 10.1021/jf010606m. [PubMed:11600050 ]
|
