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Identification
HMDB Protein ID HMDBP12767
Secondary Accession Numbers None
Name Methyl-CpG-binding domain protein 3
Synonyms
  1. Methyl-CpG-binding protein MBD3
Gene Name MBD3
Protein Type Unknown
Biological Properties
General Function Not Available
Specific Function Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
Pathways Not Available
Reactions Not Available
GO Classification
Biological Process
histone acetylation
methylation-dependent chromatin silencing
embryonic organ development
in utero embryonic development
heart development
negative regulation of transcription from RNA polymerase II promoter
ATP-dependent chromatin remodeling
brain development
response to nutrient levels
aging
response to estradiol stimulus
regulation of DNA methylation
tissue development
regulation of signal transduction by p53 class mediator
Cellular Component
heterochromatin
protein-containing complex
cytoplasm
nucleoplasm
NuRD complex
chromatin
Molecular Function
methyl-CpG binding
chromatin binding
DNA binding
Cellular Location Not Available
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues 291
Molecular Weight 32843.85
Theoretical pI 5.334
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence Not Available
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID O95983
UniProtKB/Swiss-Prot Entry Name MBD3_HUMAN
PDB IDs
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
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  3. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M: Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science. 2009 Aug 14;325(5942):834-40. doi: 10.1126/science.1175371. Epub 2009 Jul 16. [PubMed:19608861 ]
  4. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [PubMed:18669648 ]
  5. Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. [PubMed:19690332 ]
  6. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. [PubMed:17081983 ]
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  8. Hendrich B, Bird A: Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol. 1998 Nov;18(11):6538-47. [PubMed:9774669 ]
  9. Tatematsu KI, Yamazaki T, Ishikawa F: MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase. Genes Cells. 2000 Aug;5(8):677-88. [PubMed:10947852 ]
  10. Humphrey GW, Wang Y, Russanova VR, Hirai T, Qin J, Nakatani Y, Howard BH: Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1. J Biol Chem. 2001 Mar 2;276(9):6817-24. Epub 2000 Dec 1. [PubMed:11102443 ]
  11. Brackertz M, Boeke J, Zhang R, Renkawitz R: Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3. J Biol Chem. 2002 Oct 25;277(43):40958-66. Epub 2002 Aug 14. [PubMed:12183469 ]
  12. Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475. [PubMed:20068231 ]
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  15. Hendriks IA, Lyon D, Young C, Jensen LJ, Vertegaal AC, Nielsen ML: Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat Struct Mol Biol. 2017 Mar;24(3):325-336. doi: 10.1038/nsmb.3366. Epub 2017 Jan 23. [PubMed:28112733 ]
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  17. Saito M, Ishikawa F: The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2. J Biol Chem. 2002 Sep 20;277(38):35434-9. doi: 10.1074/jbc.M203455200. Epub 2002 Jul 17. [PubMed:12124384 ]
  18. Feng Q, Cao R, Xia L, Erdjument-Bromage H, Tempst P, Zhang Y: Identification and functional characterization of the p66/p68 components of the MeCP1 complex. Mol Cell Biol. 2002 Jan;22(2):536-46. doi: 10.1128/MCB.22.2.536-546.2002. [PubMed:11756549 ]
  19. Fujita N, Jaye DL, Geigerman C, Akyildiz A, Mooney MR, Boss JM, Wade PA: MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation. Cell. 2004 Oct 1;119(1):75-86. doi: 10.1016/j.cell.2004.09.014. [PubMed:15454082 ]
  20. Morey L, Brenner C, Fazi F, Villa R, Gutierrez A, Buschbeck M, Nervi C, Minucci S, Fuks F, Di Croce L: MBD3, a component of the NuRD complex, facilitates chromatin alteration and deposition of epigenetic marks. Mol Cell Biol. 2008 Oct;28(19):5912-23. doi: 10.1128/MCB.00467-08. Epub 2008 Jul 21. [PubMed:18644863 ]
  21. Gunther K, Rust M, Leers J, Boettger T, Scharfe M, Jarek M, Bartkuhn M, Renkawitz R: Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences. Nucleic Acids Res. 2013 Mar 1;41(5):3010-21. doi: 10.1093/nar/gkt035. Epub 2013 Jan 29. [PubMed:23361464 ]
  22. Shimbo T, Du Y, Grimm SA, Dhasarathy A, Mav D, Shah RR, Shi H, Wade PA: MBD3 localizes at promoters, gene bodies and enhancers of active genes. PLoS Genet. 2013;9(12):e1004028. doi: 10.1371/journal.pgen.1004028. Epub 2013 Dec 26. [PubMed:24385926 ]
  23. Cramer JM, Scarsdale JN, Walavalkar NM, Buchwald WA, Ginder GD, Williams DC Jr: Probing the dynamic distribution of bound states for methylcytosine-binding domains on DNA. J Biol Chem. 2014 Jan 17;289(3):1294-302. doi: 10.1074/jbc.M113.512236. Epub 2013 Dec 4. [PubMed:24307175 ]