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Showing Protein Polyamine transporter 1 (HMDBP12805)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 1 metabolite
Identification
HMDB Protein ID HMDBP12805
Secondary Accession Numbers None
Name Polyamine transporter 1
Synonyms Not Available
Gene Name TPO1
Protein Type Unknown
Biological Properties
General Function Not Available
Specific Function Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Catalyzes polyamine uptake at alkaline pH and excretion at acidic pH. Recognizes spermidine, spermine and putrescine, the polyamine analogs methylglyoxal bis(guanylhydrazone) (MGBG) and paraquat, the antimalarial drug quinidine, and cycloheximide. Confers resistance to the non-steroidal anti-inflammatory drug indomethacin.
Pathways Not Available
Reactions Not Available
GO Classification
Biological Process
polyamine transmembrane transport
putrescine transport
spermidine transport
spermine transport
xenobiotic detoxification by transmembrane export across the plasma membrane
Cellular Component
plasma membrane
cellular bud membrane
fungal-type vacuole membrane
cell periphery
integral to membrane
Molecular Function
xenobiotic transporter activity
spermidine transmembrane transporter activity
spermine transmembrane transporter activity
antiporter activity
Cellular Location Not Available
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues 586
Molecular Weight 64271.595
Theoretical pI 6.879
Pfam Domain Function
Signals Not Available
Transmembrane Regions
  • 146-166;182-202;213-233;243-263;272-292;302-322;377-397;415-435;455-475;484-504;506-526;529-549;550-570;
Protein Sequence Not Available
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID Q07824
UniProtKB/Swiss-Prot Entry Name TPO1_YEAST
PDB IDs Not Available
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
  1. Engel SR, Dietrich FS, Fisk DG, Binkley G, Balakrishnan R, Costanzo MC, Dwight SS, Hitz BC, Karra K, Nash RS, Weng S, Wong ED, Lloyd P, Skrzypek MS, Miyasato SR, Simison M, Cherry JM: The reference genome sequence of Saccharomyces cerevisiae: then and now. G3 (Bethesda). 2014 Mar 20;4(3):389-98. doi: 10.1534/g3.113.008995. [PubMed:24374639 ]
  2. Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS: Global analysis of protein expression in yeast. Nature. 2003 Oct 16;425(6959):737-41. doi: 10.1038/nature02046. [PubMed:14562106 ]
  3. Johnston M, Hillier L, Riles L, Albermann K, Andre B, Ansorge W, Benes V, Bruckner M, Delius H, Dubois E, Dusterhoft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, Kotter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. Nature. 1997 May 29;387(6632 Suppl):87-90. [PubMed:9169871 ]
  4. Tomitori H, Kashiwagi K, Sakata K, Kakinuma Y, Igarashi K: Identification of a gene for a polyamine transport protein in yeast. J Biol Chem. 1999 Feb 5;274(6):3265-7. doi: 10.1074/jbc.274.6.3265. [PubMed:9920864 ]
  5. Tomitori H, Kashiwagi K, Asakawa T, Kakinuma Y, Michael AJ, Igarashi K: Multiple polyamine transport systems on the vacuolar membrane in yeast. Biochem J. 2001 Feb 1;353(Pt 3):681-8. doi: 10.1042/0264-6021:3530681. [PubMed:11171066 ]
  6. do Valle Matta MA, Jonniaux JL, Balzi E, Goffeau A, van den Hazel B: Novel target genes of the yeast regulator Pdr1p: a contribution of the TPO1 gene in resistance to quinidine and other drugs. Gene. 2001 Jul 11;272(1-2):111-9. doi: 10.1016/s0378-1119(01)00558-3. [PubMed:11470516 ]
  7. Kim H, Melen K, von Heijne G: Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions. J Biol Chem. 2003 Mar 21;278(12):10208-13. doi: 10.1074/jbc.M300163200. Epub 2003 Jan 10. [PubMed:12524434 ]
  8. Albertsen M, Bellahn I, Kramer R, Waffenschmidt S: Localization and function of the yeast multidrug transporter Tpo1p. J Biol Chem. 2003 Apr 11;278(15):12820-5. doi: 10.1074/jbc.M210715200. Epub 2003 Jan 31. [PubMed:12562762 ]
  9. Shepard KA, Gerber AP, Jambhekar A, Takizawa PA, Brown PO, Herschlag D, DeRisi JL, Vale RD: Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11429-34. doi: 10.1073/pnas.2033246100. Epub 2003 Sep 17. [PubMed:13679573 ]
  10. Aranda A, del Olmo ML: Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively. Appl Environ Microbiol. 2004 Apr;70(4):1913-22. doi: 10.1128/AEM.70.4.1913-1922.2004. [PubMed:15066780 ]
  11. Uemura T, Tachihara K, Tomitori H, Kashiwagi K, Igarashi K: Characteristics of the polyamine transporter TPO1 and regulation of its activity and cellular localization by phosphorylation. J Biol Chem. 2005 Mar 11;280(10):9646-52. doi: 10.1074/jbc.M410274200. Epub 2005 Jan 6. [PubMed:15637075 ]
  12. Kim H, Melen K, Osterberg M, von Heijne G: A global topology map of the Saccharomyces cerevisiae membrane proteome. Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11142-7. doi: 10.1073/pnas.0604075103. Epub 2006 Jul 17. [PubMed:16847258 ]
  13. Mima S, Ushijima H, Hwang HJ, Tsutsumi S, Makise M, Yamaguchi Y, Tsuchiya T, Mizushima H, Mizushima T: Identification of the TPO1 gene in yeast, and its human orthologue TETRAN, which cause resistance to NSAIDs. FEBS Lett. 2007 Apr 3;581(7):1457-63. doi: 10.1016/j.febslet.2007.03.001. Epub 2007 Mar 8. [PubMed:17362938 ]
  14. Li X, Gerber SA, Rudner AD, Beausoleil SA, Haas W, Villen J, Elias JE, Gygi SP: Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae. J Proteome Res. 2007 Mar;6(3):1190-7. doi: 10.1021/pr060559j. [PubMed:17330950 ]
  15. Albuquerque CP, Smolka MB, Payne SH, Bafna V, Eng J, Zhou H: A multidimensional chromatography technology for in-depth phosphoproteome analysis. Mol Cell Proteomics. 2008 Jul;7(7):1389-96. doi: 10.1074/mcp.M700468-MCP200. Epub 2008 Apr 11. [PubMed:18407956 ]
  16. Holt LJ, Tuch BB, Villen J, Johnson AD, Gygi SP, Morgan DO: Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution. Science. 2009 Sep 25;325(5948):1682-6. doi: 10.1126/science.1172867. [PubMed:19779198 ]