Hmdb loader
Identification
HMDB Protein ID HMDBP13355
Secondary Accession Numbers None
Name Protein arginine N-methyltransferase 8
Synonyms
  1. Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4
Gene Name PRMT8
Protein Type Unknown
Biological Properties
General Function Not Available
Specific Function S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA) in proteins such as NIFK, myelin basic protein, histone H4, H2A and H2A/H2B dimer (PubMed:16051612, PubMed:17925405, PubMed:26876602, PubMed:26529540). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS (PubMed:18320585).
Pathways Not Available
Reactions Not Available
GO Classification
Biological Process
histone methylation
peptidyl-arginine methylation
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
protein methylation
protein homooligomerization
regulation of protein binding
Cellular Component
anchored component of the cytoplasmic side of the plasma membrane
plasma membrane
Molecular Function
S-adenosyl-L-methionine binding
S-adenosylmethionine-dependent methyltransferase activity
histone-arginine N-methyltransferase activity
protein homodimerization activity
protein-arginine omega-N asymmetric methyltransferase activity
protein-arginine omega-N monomethyltransferase activity
enzyme binding
identical protein binding
Cellular Location Not Available
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues 394
Molecular Weight 45290.845
Theoretical pI 6.933
Pfam Domain Function Not Available
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence Not Available
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID Q9NR22
UniProtKB/Swiss-Prot Entry Name ANM8_HUMAN
PDB IDs
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
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  4. Sayegh J, Webb K, Cheng D, Bedford MT, Clarke SG: Regulation of protein arginine methyltransferase 8 (PRMT8) activity by its N-terminal domain. J Biol Chem. 2007 Dec 14;282(50):36444-53. Epub 2007 Oct 9. [PubMed:17925405 ]
  5. Pahlich S, Zakaryan RP, Gehring H: Identification of proteins interacting with protein arginine methyltransferase 8: the Ewing sarcoma (EWS) protein binds independent of its methylation state. Proteins. 2008 Sep;72(4):1125-37. doi: 10.1002/prot.22004. [PubMed:18320585 ]
  6. Lee J, Sayegh J, Daniel J, Clarke S, Bedford MT: PRMT8, a new membrane-bound tissue-specific member of the protein arginine methyltransferase family. J Biol Chem. 2005 Sep 23;280(38):32890-6. doi: 10.1074/jbc.M506944200. Epub 2005 Jul 28. [PubMed:16051612 ]
  7. Toma-Fukai S, Kim JD, Park KE, Kuwabara N, Shimizu N, Krayukhina E, Uchiyama S, Fukamizu A, Shimizu T: Novel helical assembly in arginine methyltransferase 8. J Mol Biol. 2016 Mar 27;428(6):1197-1208. doi: 10.1016/j.jmb.2016.02.007. Epub 2016 Feb 11. [PubMed:26876602 ]
  8. Lee WC, Lin WL, Matsui T, Chen ES, Wei TY, Lin WH, Hu H, Zheng YG, Tsai MD, Ho MC: Protein Arginine Methyltransferase 8: Tetrameric Structure and Protein Substrate Specificity. Biochemistry. 2015 Dec 29;54(51):7514-23. doi: 10.1021/acs.biochem.5b00995. Epub 2015 Dec 15. [PubMed:26529540 ]