| Identification |
|---|
| HMDB Protein ID
| HMDBP13367 |
| Secondary Accession Numbers
| None |
| Name
| Cytochrome P450 monooxygenase ecdH |
| Synonyms
|
- Echinocandin B biosynthetic cluster protein H
|
| Gene Name
| ECDH |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of echinocandin B, a fungal lipidated cyclic hexapeptide that acts as an antifungal agent (PubMed:22998630). Linoleoyl-AMP, produced by the fatty-acyl-AMP ligase ecdI, is transferred to the initiation carrier domain (T0) of ecdA (PubMed:22998630). The linoleoyl-S-phosphopantetheinyl-T0 is sequentially extended with L-ornithine, L-threonine, L-proline, L-homotyrosine, L-threonine, and 4R-methyl-L-proline to form the linear hexapeptide (PubMed:22998630). Thereafter, the terminal condensation (C7) performs macrocyclization of the NRPS product and the cyclic scaffold is released from ecdA (PubMed:22998630). All six of the amino acid residues are hydroxylated, including 4R,5R-dihydroxy-L-ornithine, 4R-hydroxyl-L-proline, 3S,4S-dihydroxy-L-homotyrosine, and 3S-hydroxyl-4S-methyl-L-prolin (PubMed:22998630). In the pathway, all the hydroxylation reactions are proposed to occur following completion of the cyclic peptide, so the unhydroxylated precursor produced by ecdA will undergo six rounds of hydroxylation (PubMed:22998630). Five hydroxylase genes (ecdG, ecdH, ecdK, htyE and htyF) are embedded within the echinocandin B (ecd) and L-homotyrosine (hty) clusters (PubMed:22998630). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Cellular Component |
| integral to membrane |
| Molecular Function |
| monooxygenase activity |
| iron ion binding |
| oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| heme binding |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 56634.25 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| K0E684 |
| UniProtKB/Swiss-Prot Entry Name
| ECDH_ASPRU |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Cacho RA, Jiang W, Chooi YH, Walsh CT, Tang Y: Identification and characterization of the echinocandin B biosynthetic gene cluster from Emericella rugulosa NRRL 11440. J Am Chem Soc. 2012 Oct 10;134(40):16781-90. doi: 10.1021/ja307220z. Epub 2012 Oct 1. [PubMed:22998630 ]
|
