| Identification |
|---|
| HMDB Protein ID
| HMDBP13407 |
| Secondary Accession Numbers
| None |
| Name
| Serine/threonine-protein kinase US3 homolog |
| Synonyms
|
- Protein kinase ORF66
|
| Gene Name
| 66 |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Multifunctional serine/threonine kinase that plays a role in several processes including egress of virus particles from the nucleus, modulation of the actin cytoskeleton and inhibition of apoptosis. Phosphorylates proteins 24 and 27, two critical regulators of capsid budding from nucleus to endoplasmic reticulum, thereby facilitating virion egress. Modulates and redistributes host components of the nuclear envelope, including LMNA, emerin/EMD and the nuclear matrix protein MATR3. Phosphorylates envelope glycoprotein B (gB), probably to direct it to the cell surface. Promotes virus intracellular spread by restructuring host cell cytoskeleton. Blocks host apoptosis to extend cell survival and allow efficient viral replication. Promotes viral gene expression by phosphorylating host HDAC2 to reduce viral genome silencing (By similarity). Downregulates class I major histocompatibility complex (MHC-I) surface expression. Additionally, phosphorylates IE62 and targets it to the cytoplasm. The nuclear exclusion of IE62 enables the packaging of abundant levels of IE62 into virions. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| modulation by virus of host apoptotic process |
| modulation by virus of host chromatin organization |
| suppression by virus of host adaptive immune response |
| suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I |
| Cellular Component |
| host cell cytoplasm |
| host cell nucleus |
| Molecular Function |
| ATP binding |
| protein serine kinase activity |
| protein threonine kinase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 393 |
| Molecular Weight
| 43679.27 |
| Theoretical pI
| 6.093 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| P09251 |
| UniProtKB/Swiss-Prot Entry Name
| US03_VZVD |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Davison AJ, Scott JE: The complete DNA sequence of varicella-zoster virus. J Gen Virol. 1986 Sep;67 ( Pt 9):1759-816. doi: 10.1099/0022-1317-67-9-1759. [PubMed:3018124 ]
- Davison AJ: DNA sequence of the US component of the varicella-zoster virus genome. EMBO J. 1983;2(12):2203-9. [PubMed:6321154 ]
- Leader DP, Purves FC: The herpesvirus protein kinase: a new departure in protein phosphorylation? Trends Biochem Sci. 1988 Jul;13(7):244-6. doi: 10.1016/0968-0004(88)90157-0. [PubMed:2855680 ]
- Kinchington PR, Fite K, Seman A, Turse SE: Virion association of IE62, the varicella-zoster virus (VZV) major transcriptional regulatory protein, requires expression of the VZV open reading frame 66 protein kinase. J Virol. 2001 Oct;75(19):9106-13. doi: 10.1128/JVI.75.19.9106-9113.2001. [PubMed:11533174 ]
- Eisfeld AJ, Turse SE, Jackson SA, Lerner EC, Kinchington PR: Phosphorylation of the varicella-zoster virus (VZV) major transcriptional regulatory protein IE62 by the VZV open reading frame 66 protein kinase. J Virol. 2006 Feb;80(4):1710-23. doi: 10.1128/JVI.80.4.1710-1723.2006. [PubMed:16439528 ]
- Eisfeld AJ, Yee MB, Erazo A, Abendroth A, Kinchington PR: Downregulation of class I major histocompatibility complex surface expression by varicella-zoster virus involves open reading frame 66 protein kinase-dependent and -independent mechanisms. J Virol. 2007 Sep;81(17):9034-49. doi: 10.1128/JVI.00711-07. Epub 2007 Jun 13. [PubMed:17567702 ]
- Erazo A, Yee MB, Banfield BW, Kinchington PR: The alphaherpesvirus US3/ORF66 protein kinases direct phosphorylation of the nuclear matrix protein matrin 3. J Virol. 2011 Jan;85(1):568-81. doi: 10.1128/JVI.01611-10. Epub 2010 Oct 20. [PubMed:20962082 ]
|
