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Showing Protein Polypeptide N-acetylgalactosaminyltransferase 35A (HMDBP13965)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 0 metabolites
Identification
HMDB Protein ID HMDBP13965
Secondary Accession Numbers None
Name Polypeptide N-acetylgalactosaminyltransferase 35A
Synonyms
  1. Protein l(2)35Aa
  2. Protein-UDP acetylgalactosaminyltransferase 35A
  3. UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 35A
  4. dGalNAc-T1
  5. pp-GaNTase 35A
Gene Name PGANT35A
Protein Type Unknown
Biological Properties
General Function Not Available
Specific Function Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor (PubMed:11925450, PubMed:12829714). Displays the same enzyme activity toward MUC1, MUC4, and EA2 (PubMed:11925450, PubMed:12829714). Not involved in glycosylation of erythropoietin (EPO) (PubMed:11925450). It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties (PubMed:11925450, PubMed:12829714). Protein modification by this enzyme might be important for cytokinesis and tube formation during embryogenesis (PubMed:16251381, PubMed:20807760). Together with Pgant3, regulates integrin levels and activity-dependent integrin signaling at the synapse in neurons and muscles (PubMed:25253852).
Pathways
  • Mucin type O-glycan biosynthesis
  • Other types of O-glycan biosynthesis
  • protein glycosylation
Reactions Not Available
GO Classification
Biological Process
oligosaccharide biosynthetic process
open tracheal system development
regulation of Notch signaling pathway
protein glycosylation
Cellular Component
Golgi apparatus
Golgi stack
integral to membrane
Golgi membrane
Molecular Function
Notch binding
metal ion binding
carbohydrate binding
polypeptide N-acetylgalactosaminyltransferase activity
Cellular Location Not Available
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues 632
Molecular Weight 71827.745
Theoretical pI 8.816
Pfam Domain Function
Signals Not Available
Transmembrane Regions
  • 7-29;
Protein Sequence Not Available
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID Q8MVS5
UniProtKB/Swiss-Prot Entry Name GLT35_DROME
PDB IDs Not Available
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
  1. Schwientek T, Bennett EP, Flores C, Thacker J, Hollmann M, Reis CA, Behrens J, Mandel U, Keck B, Schafer MA, Haselmann K, Zubarev R, Roepstorff P, Burchell JM, Taylor-Papadimitriou J, Hollingsworth MA, Clausen H: Functional conservation of subfamilies of putative UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases in Drosophila, Caenorhabditis elegans, and mammals. One subfamily composed of l(2)35Aa is essential in Drosophila. J Biol Chem. 2002 Jun 21;277(25):22623-38. Epub 2002 Mar 29. [PubMed:11925450 ]
  2. Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF, George RA, Lewis SE, Richards S, Ashburner M, Henderson SN, Sutton GG, Wortman JR, Yandell MD, Zhang Q, Chen LX, Brandon RC, Rogers YH, Blazej RG, Champe M, Pfeiffer BD, Wan KH, Doyle C, Baxter EG, Helt G, Nelson CR, Gabor GL, Abril JF, Agbayani A, An HJ, Andrews-Pfannkoch C, Baldwin D, Ballew RM, Basu A, Baxendale J, Bayraktaroglu L, Beasley EM, Beeson KY, Benos PV, Berman BP, Bhandari D, Bolshakov S, Borkova D, Botchan MR, Bouck J, Brokstein P, Brottier P, Burtis KC, Busam DA, Butler H, Cadieu E, Center A, Chandra I, Cherry JM, Cawley S, Dahlke C, Davenport LB, Davies P, de Pablos B, Delcher A, Deng Z, Mays AD, Dew I, Dietz SM, Dodson K, Doup LE, Downes M, Dugan-Rocha S, Dunkov BC, Dunn P, Durbin KJ, Evangelista CC, Ferraz C, Ferriera S, Fleischmann W, Fosler C, Gabrielian AE, Garg NS, Gelbart WM, Glasser K, Glodek A, Gong F, Gorrell JH, Gu Z, Guan P, Harris M, Harris NL, Harvey D, Heiman TJ, Hernandez JR, Houck J, Hostin D, Houston KA, Howland TJ, Wei MH, Ibegwam C, Jalali M, Kalush F, Karpen GH, Ke Z, Kennison JA, Ketchum KA, Kimmel BE, Kodira CD, Kraft C, Kravitz S, Kulp D, Lai Z, Lasko P, Lei Y, Levitsky AA, Li J, Li Z, Liang Y, Lin X, Liu X, Mattei B, McIntosh TC, McLeod MP, McPherson D, Merkulov G, Milshina NV, Mobarry C, Morris J, Moshrefi A, Mount SM, Moy M, Murphy B, Murphy L, Muzny DM, Nelson DL, Nelson DR, Nelson KA, Nixon K, Nusskern DR, Pacleb JM, Palazzolo M, Pittman GS, Pan S, Pollard J, Puri V, Reese MG, Reinert K, Remington K, Saunders RD, Scheeler F, Shen H, Shue BC, Siden-Kiamos I, Simpson M, Skupski MP, Smith T, Spier E, Spradling AC, Stapleton M, Strong R, Sun E, Svirskas R, Tector C, Turner R, Venter E, Wang AH, Wang X, Wang ZY, Wassarman DA, Weinstock GM, Weissenbach J, Williams SM, WoodageT, Worley KC, Wu D, Yang S, Yao QA, Ye J, Yeh RF, Zaveri JS, Zhan M, Zhang G, Zhao Q, Zheng L, Zheng XH, Zhong FN, Zhong W, Zhou X, Zhu S, Zhu X, Smith HO, Gibbs RA, Myers EW, Rubin GM, Venter JC: The genome sequence of Drosophila melanogaster. Science. 2000 Mar 24;287(5461):2185-95. doi: 10.1126/science.287.5461.2185. [PubMed:10731132 ]
  3. Misra S, Crosby MA, Mungall CJ, Matthews BB, Campbell KS, Hradecky P, Huang Y, Kaminker JS, Millburn GH, Prochnik SE, Smith CD, Tupy JL, Whitfied EJ, Bayraktaroglu L, Berman BP, Bettencourt BR, Celniker SE, de Grey AD, Drysdale RA, Harris NL, Richter J, Russo S, Schroeder AJ, Shu SQ, Stapleton M, Yamada C, Ashburner M, Gelbart WM, Rubin GM, Lewis SE: Annotation of the Drosophila melanogaster euchromatic genome: a systematic review. Genome Biol. 2002;3(12):RESEARCH0083. doi: 10.1186/gb-2002-3-12-research0083. Epub 2002 Dec 31. [PubMed:12537572 ]
  4. Stapleton M, Carlson J, Brokstein P, Yu C, Champe M, George R, Guarin H, Kronmiller B, Pacleb J, Park S, Wan K, Rubin GM, Celniker SE: A Drosophila full-length cDNA resource. Genome Biol. 2002;3(12):RESEARCH0080. doi: 10.1186/gb-2002-3-12-research0080. Epub 2002 Dec 23. [PubMed:12537569 ]
  5. Ten Hagen KG, Tran DT: A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is essential for viability in Drosophila melanogaster. J Biol Chem. 2002 Jun 21;277(25):22616-22. doi: 10.1074/jbc.M201807200. Epub 2002 Mar 29. [PubMed:11925446 ]
  6. Ashburner M, Misra S, Roote J, Lewis SE, Blazej R, Davis T, Doyle C, Galle R, George R, Harris N, Hartzell G, Harvey D, Hong L, Houston K, Hoskins R, Johnson G, Martin C, Moshrefi A, Palazzolo M, Reese MG, Spradling A, Tsang G, Wan K, Whitelaw K, Celniker S, et al.: An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region. Genetics. 1999 Sep;153(1):179-219. doi: 10.1093/genetics/153.1.179. [PubMed:10471707 ]
  7. Ten Hagen KG, Tran DT, Gerken TA, Stein DS, Zhang Z: Functional characterization and expression analysis of members of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family from Drosophila melanogaster. J Biol Chem. 2003 Sep 12;278(37):35039-48. doi: 10.1074/jbc.M303836200. Epub 2003 Jun 26. [PubMed:12829714 ]
  8. Tian E, Ten Hagen KG: Expression of the UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferase family is spatially and temporally regulated during Drosophila development. Glycobiology. 2006 Feb;16(2):83-95. doi: 10.1093/glycob/cwj051. Epub 2005 Oct 26. [PubMed:16251381 ]
  9. Koles K, Lim JM, Aoki K, Porterfield M, Tiemeyer M, Wells L, Panin V: Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster. Glycobiology. 2007 Dec;17(12):1388-403. doi: 10.1093/glycob/cwm097. Epub 2007 Sep 23. [PubMed:17893096 ]
  10. Bennett EP, Chen YW, Schwientek T, Mandel U, Schjoldager Kt, Cohen SM, Clausen H: Rescue of Drosophila Melanogaster l(2)35Aa lethality is only mediated by polypeptide GalNAc-transferase pgant35A, but not by the evolutionary conserved human ortholog GalNAc-transferase-T11. Glycoconj J. 2010 May;27(4):435-44. doi: 10.1007/s10719-010-9290-5. Epub 2010 Apr 27. [PubMed:20422447 ]
  11. Zhang L, Ten Hagen KG: Dissecting the biological role of mucin-type O-glycosylation using RNA interference in Drosophila cell culture. J Biol Chem. 2010 Nov 5;285(45):34477-84. doi: 10.1074/jbc.M110.133561. Epub 2010 Aug 31. [PubMed:20807760 ]
  12. Dani N, Zhu H, Broadie K: Two protein N-acetylgalactosaminyl transferases regulate synaptic plasticity by activity-dependent regulation of integrin signaling. J Neurosci. 2014 Sep 24;34(39):13047-65. doi: 10.1523/JNEUROSCI.1484-14.2014. [PubMed:25253852 ]