| Identification |
|---|
| HMDB Protein ID
| HMDBP13968 |
| Secondary Accession Numbers
| None |
| Name
| Beta-hexosaminidase |
| Synonyms
|
- AoHEX
- Beta-N-acetylglucosaminidase
- Beta-N-acetylhexosaminidase
- Hex
|
| Gene Name
| NAGA |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Selectively hydrolyzes GlcNAcbeta(1->4)GlcNAc (N,N'-diacetylchitobiose) and Gal-NAcbeta(1->4)GlcNAc, but not their C-2 epimers GlcNAcbeta(1->4)ManNAc or Gal-NAcbeta(1->4)ManNAc. However, hydrolyzes both GlcNAcbeta(1->6)GlcNAc and GlcNAcbeta(1->6)ManNAc (Ref.7). Part of the binary chitinolytic system. Involved in hydrolyzation of chitobiose and higher chito-oligomers (produced from cell wall chitin by endochitinases), thus contributing to the formation of germ tubes, fruit-bodies and septa during hyphenation (Probable). Hydrolyzes synthetic substrate p-nitrophenyl-beta-N-acetyl-D-glucosaminide (pNP-GlcNAc) (PubMed:17302431, PubMed:12723619, Ref.7). Hydrolyzes synthetic substrate p-nitrophenyl-beta-N-acetyl-D-galactosaminide (pNP-GalNAc) (PubMed:12723619). Hydrolyzes chromogenic substrate 4-nitrophenyl-2-acetamido-2-deoxyglucopyranoside (PubMed:21505251, PubMed:17509134, PubMed:29239122). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| carbohydrate metabolic process |
| Molecular Function |
| N-acetyl-beta-D-galactosaminidase activity |
| beta-N-acetylhexosaminidase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 67524.855 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| Q8J2T0 |
| UniProtKB/Swiss-Prot Entry Name
| HEXA_ASPOZ |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Plihal O, Sklenar J, Kmonickova J, Man P, Pompach P, Havlicek V, Kren V, Bezouska K: N-glycosylated catalytic unit meets O-glycosylated propeptide: complex protein architecture in a fungal hexosaminidase. Biochem Soc Trans. 2004 Nov;32(Pt 5):764-5. doi: 10.1042/BST0320764. [PubMed:15494009 ]
- Plihal O, Sklenar J, Hofbauerova K, Novak P, Man P, Pompach P, Kavan D, Ryslava H, Weignerova L, Charvatova-Pisvejcova A, Kren V, Bezouska K: Large propeptides of fungal beta-N-acetylhexosaminidases are novel enzyme regulators that must be intracellularly processed to control activity, dimerization, and secretion into the extracellular environment. Biochemistry. 2007 Mar 13;46(10):2719-34. doi: 10.1021/bi061828m. Epub 2007 Feb 16. [PubMed:17302431 ]
- Matsuo I, Kim S, Yamamoto Y, Ajisaka K, Maruyama JI, Nakajima H, Kitamoto K: Cloning and overexpression of beta-N-acetylglucosaminidase encoding gene nagA from Aspergillus oryzae and enzyme-catalyzed synthesis of human milk oligosaccharide. Biosci Biotechnol Biochem. 2003 Mar;67(3):646-50. doi: 10.1271/bbb.67.646. [PubMed:12723619 ]
- Thammarongtham C, Nookaew I, Vorapreeda T, Srisuk T, Land ML, Jeennor S, Laoteng K: Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production. Curr Microbiol. 2018 Jan;75(1):57-70. doi: 10.1007/s00284-017-1350-7. Epub 2017 Sep 1. [PubMed:28865010 ]
- Ettrich R, Kopecky V Jr, Hofbauerova K, Baumruk V, Novak P, Pompach P, Man P, Plihal O, Kuty M, Kulik N, Sklenar J, Ryslava H, Kren V, Bezouska K: Structure of the dimeric N-glycosylated form of fungal beta-N-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies. BMC Struct Biol. 2007 May 17;7:32. doi: 10.1186/1472-6807-7-32. [PubMed:17509134 ]
- Weignerova L, Vavruskova P, Pisvejcova A, Thiem J, Kren V: Fungal beta-N-acetylhexosaminidases with high beta-N-acetylgalactosaminidase activity and their use for synthesis of beta-GalNAc-containing oligosaccharides. Carbohydr Res. 2003 Apr 22;338(9):1003-8. doi: 10.1016/s0008-6215(03)00044-2. [PubMed:12681926 ]
- Skerlova J, Blaha J, Pachl P, Hofbauerova K, Kukacka Z, Man P, Pompach P, Novak P, Otwinowski Z, Brynda J, Vanek O, Rezacova P: Crystal structure of native beta-N-acetylhexosaminidase isolated from Aspergillus oryzae sheds light onto its substrate specificity, high stability, and regulation by propeptide. FEBS J. 2018 Feb;285(3):580-598. doi: 10.1111/febs.14360. Epub 2017 Dec 30. [PubMed:29239122 ]
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