Hmdb loader
You are using an unsupported browser. Please upgrade your browser to a newer version to get the best experience on Human Metabolome Database.
Identification
HMDB Protein ID HMDBP14357
Secondary Accession Numbers None
Name Glycophorin-A
Synonyms
  1. MN sialoglycoprotein
  2. PAS-2
  3. Sialoglycoprotein alpha
Gene Name GYPA
Protein Type Unknown
Biological Properties
General Function Not Available
Specific Function Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
Pathways
  • Hematopoietic cell lineage
  • Malaria
Reactions Not Available
GO Classification
Biological Process
leukocyte migration
Cellular Component
cytosol
plasma membrane
nucleoplasm
membrane
integral to plasma membrane
Molecular Function
viral receptor activity
identical protein binding
Cellular Location Not Available
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues 117
Molecular Weight 16331.305
Theoretical pI 5.228
Pfam Domain Function Not Available
Signals
  • 1-19;
Transmembrane Regions
  • 92-114;
Protein Sequence Not Available
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID P02724
UniProtKB/Swiss-Prot Entry Name GLPA_HUMAN
PDB IDs
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
  1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [PubMed:14702039 ]
  2. Hillier LW, Graves TA, Fulton RS, Fulton LA, Pepin KH, Minx P, Wagner-McPherson C, Layman D, Wylie K, Sekhon M, Becker MC, Fewell GA, Delehaunty KD, Miner TL, Nash WE, Kremitzki C, Oddy L, Du H, Sun H, Bradshaw-Cordum H, Ali J, Carter J, Cordes M, Harris A, Isak A, van Brunt A, Nguyen C, Du F, Courtney L, Kalicki J, Ozersky P, Abbott S, Armstrong J, Belter EA, Caruso L, Cedroni M, Cotton M, Davidson T, Desai A, Elliott G, Erb T, Fronick C, Gaige T, Haakenson W, Haglund K, Holmes A, Harkins R, Kim K, Kruchowski SS, Strong CM, Grewal N, Goyea E, Hou S, Levy A, Martinka S, Mead K, McLellan MD, Meyer R, Randall-Maher J, Tomlinson C, Dauphin-Kohlberg S, Kozlowicz-Reilly A, Shah N, Swearengen-Shahid S, Snider J, Strong JT, Thompson J, Yoakum M, Leonard S, Pearman C, Trani L, Radionenko M, Waligorski JE, Wang C, Rock SM, Tin-Wollam AM, Maupin R, Latreille P, Wendl MC, Yang SP, Pohl C, Wallis JW, Spieth J, Bieri TA, Berkowicz N, Nelson JO, Osborne J, Ding L, Meyer R, Sabo A, Shotland Y, Sinha P, Wohldmann PE, Cook LL, Hickenbotham MT, Eldred J, Williams D, Jones TA, She X, Ciccarelli FD, Izaurralde E, Taylor J, Schmutz J, Myers RM, Cox DR, Huang X, McPherson JD, Mardis ER, Clifton SW, Warren WC, Chinwalla AT, Eddy SR, Marra MA, Ovcharenko I, Furey TS, Miller W, Eichler EE, Bork P, Suyama M, Torrents D, Waterston RH, Wilson RK: Generation and annotation of the DNA sequences of human chromosomes 2 and 4. Nature. 2005 Apr 7;434(7034):724-31. [PubMed:15815621 ]
  3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  4. Kudo S, Fukuda M: Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4619-23. [PubMed:2734312 ]
  5. Siebert PD, Fukuda M: Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1665-9. [PubMed:3456608 ]
  6. Tate CG, Tanner MJ: Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta. Biochem J. 1988 Sep 15;254(3):743-50. [PubMed:3196288 ]
  7. Hamid J, Burness AT: The mechanism of production of multiple mRNAs for human glycophorin A. Nucleic Acids Res. 1990 Oct 11;18(19):5829-36. [PubMed:2216775 ]
  8. Kudo S, Onda M, Fukuda M: Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals. J Biochem. 1994 Jul;116(1):183-92. [PubMed:7798177 ]
  9. Siebert PD, Fukuda M: Molecular biological study of the structure and expression of human glycophorin A. Rev Fr Transfus Immunohematol. 1986 Sep;29(4):251-66. [PubMed:3809885 ]
  10. Rahuel C, London J, d'Auriol L, Mattei MG, Tournamille C, Skrzynia C, Lebouc Y, Galibert F, Cartron JP: Characterization of cDNA clones for human glycophorin A. Use for gene localization and for analysis of normal of glycophorin-A-deficient (Finnish type) genomic DNA. Eur J Biochem. 1988 Feb 15;172(1):147-53. [PubMed:3345758 ]
  11. Tomita M, Marchesi VT: Amino-acid sequence and oligosaccharide attachment sites of human erythrocyte glycophorin. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2964-8. [PubMed:1059087 ]
  12. Dill K, Hu SH, Berman E, Pavia AA, Lacombe JM: One- and two-dimensional NMR studies of the N-terminal portion of glycophorin A at 11.7 Tesla. J Protein Chem. 1990 Apr;9(2):129-36. [PubMed:2386609 ]
  13. MacKenzie KR, Prestegard JH, Engelman DM: A transmembrane helix dimer: structure and implications. Science. 1997 Apr 4;276(5309):131-3. [PubMed:9082985 ]
  14. Adams PD, Engelman DM, Brunger AT: Improved prediction for the structure of the dimeric transmembrane domain of glycophorin A obtained through global searching. Proteins. 1996 Nov;26(3):257-61. [PubMed:8953647 ]
  15. Pisano A, Redmond JW, Williams KL, Gooley AA: Glycosylation sites identified by solid-phase Edman degradation: O-linked glycosylation motifs on human glycophorin A. Glycobiology. 1993 Oct;3(5):429-35. [PubMed:8286855 ]
  16. Huang CH, Spruell P, Moulds JJ, Blumenfeld OO: Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype. Blood. 1992 Jul 1;80(1):257-63. [PubMed:1611092 ]
  17. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [PubMed:24275569 ]
  18. Furthmayr H, Metaxas MN, Metaxas-Buhler M: Mg and Mc: mutations within the amino-terminal region of glycophorin A. Proc Natl Acad Sci U S A. 1981 Jan;78(1):631-5. doi: 10.1073/pnas.78.1.631. [PubMed:6166001 ]
  19. Blumenfeld OO, Adamany AM, Puglia KV: Amino acid and carbohydrate structural variants of glycoprotein products (M-N glycoproteins) of the M-N allelic locus. Proc Natl Acad Sci U S A. 1981 Feb;78(2):747-51. doi: 10.1073/pnas.78.2.747. [PubMed:6940143 ]
  20. Thomas DB, Winzler RJ: Structural studies on human erythrocyte glycoproteins. Alkali-labile oligosaccharides. J Biol Chem. 1969 Nov 10;244(21):5943-6. [PubMed:5350948 ]
  21. Pasvol G, Wainscoat JS, Weatherall DJ: Erythrocytes deficiency in glycophorin resist invasion by the malarial parasite Plasmodium falciparum. Nature. 1982 May 6;297(5861):64-6. doi: 10.1038/297064a0. [PubMed:7040988 ]
  22. Fukuda M, Lauffenburger M, Sasaki H, Rogers ME, Dell A: Structures of novel sialylated O-linked oligosaccharides isolated from human erythrocyte glycophorins. J Biol Chem. 1987 Sep 5;262(25):11952-7. [PubMed:3624241 ]
  23. Treutlein HR, Lemmon MA, Engelman DM, Brunger AT: The glycophorin A transmembrane domain dimer: sequence-specific propensity for a right-handed supercoil of helices. Biochemistry. 1992 Dec 29;31(51):12726-32. doi: 10.1021/bi00166a003. [PubMed:1463744 ]
  24. Sim BK, Chitnis CE, Wasniowska K, Hadley TJ, Miller LH: Receptor and ligand domains for invasion of erythrocytes by Plasmodium falciparum. Science. 1994 Jun 24;264(5167):1941-4. doi: 10.1126/science.8009226. [PubMed:8009226 ]
  25. Podbielska M, Krotkiewski H: Identification of blood group A and B antigens in human glycophorin. Arch Immunol Ther Exp (Warsz). 2000;48(3):211-21. [PubMed:10912628 ]
  26. Young MT, Beckmann R, Toye AM, Tanner MJ: Red-cell glycophorin A-band 3 interactions associated with the movement of band 3 to the cell surface. Biochem J. 2000 Aug 15;350 Pt 1:53-60. [PubMed:10926825 ]
  27. Gerber D, Shai Y: In vivo detection of hetero-association of glycophorin-A and its mutants within the membrane. J Biol Chem. 2001 Aug 17;276(33):31229-32. doi: 10.1074/jbc.M101889200. Epub 2001 Jun 11. [PubMed:11402026 ]
  28. Young MT, Tanner MJ: Distinct regions of human glycophorin A enhance human red cell anion exchanger (band 3; AE1) transport function and surface trafficking. J Biol Chem. 2003 Aug 29;278(35):32954-61. doi: 10.1074/jbc.M302527200. Epub 2003 Jun 17. [PubMed:12813056 ]
  29. Podbielska M, Fredriksson SA, Nilsson B, Lisowska E, Krotkiewski H: ABH blood group antigens in O-glycans of human glycophorin A. Arch Biochem Biophys. 2004 Sep 15;429(2):145-53. doi: 10.1016/j.abb.2004.06.018. [PubMed:15313217 ]
  30. Bruce LJ, Pan RJ, Cope DL, Uchikawa M, Gunn RB, Cherry RJ, Tanner MJ: Altered structure and anion transport properties of band 3 (AE1, SLC4A1) in human red cells lacking glycophorin A. J Biol Chem. 2004 Jan 23;279(4):2414-20. doi: 10.1074/jbc.M309826200. Epub 2003 Nov 5. [PubMed:14604989 ]
  31. Sanchez G, Aragones L, Costafreda MI, Ribes E, Bosch A, Pinto RM: Capsid region involved in hepatitis A virus binding to glycophorin A of the erythrocyte membrane. J Virol. 2004 Sep;78(18):9807-13. doi: 10.1128/JVI.78.18.9807-9813.2004. [PubMed:15331714 ]
  32. Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008 Feb;52(2):69-77. doi: 10.1111/j.1348-0421.2008.00015.x. [PubMed:18380804 ]
  33. Pang AJ, Reithmeier RA: Interaction of anion exchanger 1 and glycophorin A in human erythroleukaemic K562 cells. Biochem J. 2009 Jul 15;421(3):345-56. doi: 10.1042/BJ20090345. [PubMed:19438409 ]
  34. Huang CH, Skov F, Daniels G, Tippett P, Blumenfeld OO: Molecular analysis of human glycophorin MiIX gene shows a silent segment transfer and untemplated mutation resulting from gene conversion via sequence repeats. Blood. 1992 Nov 1;80(9):2379-87. [PubMed:1421409 ]
  35. Huang CH, Reid M, Daniels G, Blumenfeld OO: Alteration of splice site selection by an exon mutation in the human glycophorin A gene. J Biol Chem. 1993 Dec 5;268(34):25902-8. [PubMed:8245024 ]
  36. Poole J, Banks J, Bruce LJ, Ring SM, Levene C, Stern H, Overbeeke MA, Tanner MJ: Glycophorin A mutation Ala65 --> Pro gives rise to a novel pair of MNS alleles ENEP (MNS39) and HAG (MNS41) and altered Wrb expression: direct evidence for GPA/band 3 interaction necessary for normal Wrb expression. Transfus Med. 1999 Jun;9(2):167-74. doi: 10.1046/j.1365-3148.1999.00185.x. [PubMed:10354388 ]
  37. Daniels GL, Bruce LJ, Mawby WJ, Green CA, Petty A, Okubo Y, Kornstad L, Tanner MJ: The low-frequency MNS blood group antigens Ny(a) (MNS18) and Os(a) (MNS38) are associated with GPA amino acid substitutions. Transfusion. 2000 May;40(5):555-9. doi: 10.1046/j.1537-2995.2000.40050555.x. [PubMed:10827258 ]