| Identification |
|---|
| HMDB Protein ID
| HMDBP14451 |
| Secondary Accession Numbers
| None |
| Name
| 6-methylsalicylic acid synthase |
| Synonyms
|
- 6-MSAS
- Polyketide synthase atX
- Terreic acid biosynthesis protein X
|
| Gene Name
| ATX |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| 6-methylsalicylic acid synthase; part of the gene cluster that mediates the biosynthesis of terreic acid, a quinone epoxide inhibitor of Bruton's tyrosine kinase (PubMed:24534845, PubMed:25265334). The first step of the pathway is the synthesis of 6-methylsalicylic acid (6-MSA) by the 6-methylsalicylic acid synthase atX (PubMed:9003280, PubMed:9438344, PubMed:25265334). In the biosynthesis of 6-MSA, atX utilizes one acetyl-CoA and three malonyl-CoAs as its substrates and catalyzes a series of programmed reactions including Claisen condensation, dehydration, reduction, and cyclization to yield 6-MSA (PubMed:9003280, PubMed:9438344, PubMed:25265334). The 6-methylsalicylic acid decarboxylase atA then catalyzes the decarboxylative hydroxylation of 6-MSA to 3-methylcatechol (PubMed:25265334). The next step is the conversion of 3-methylcatechol to terremutin via several oxidation steps involving the cytochrome P450 monooxygenase atE and probably also the cytochrome P450 monooxygenase atG (PubMed:25265334). Lastly, atC is required for the oxidation of terremutin to terreic acid (PubMed:25265334). No function could be assigned to atD yet, although it is involved in the biosynthesis of terreic acid (PubMed:25265334). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| fatty acid biosynthetic process |
| Molecular Function |
| oxidoreductase activity |
| 6-methylsalicylic acid synthase activity |
| phosphopantetheine binding |
| 3-oxoacyl-[acyl-carrier-protein] synthase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 193792.24 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| Q0CJ59 |
| UniProtKB/Swiss-Prot Entry Name
| ATX_ASPTN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Fujii I, Ono Y, Tada H, Gomi K, Ebizuka Y, Sankawa U: Cloning of the polyketide synthase gene atX from Aspergillus terreus and its identification as the 6-methylsalicylic acid synthase gene by heterologous expression. Mol Gen Genet. 1996 Nov 27;253(1-2):1-10. doi: 10.1007/s004380050289. [PubMed:9003280 ]
- Pazoutova S, Linka M, Storkova S, Schwab H: Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase. Folia Microbiol (Praha). 1997;42(5):419-30. doi: 10.1007/BF02826548. [PubMed:9438344 ]
- Kawakami Y, Hartman SE, Kinoshita E, Suzuki H, Kitaura J, Yao L, Inagaki N, Franco A, Hata D, Maeda-Yamamoto M, Fukamachi H, Nagai H, Kawakami T: Terreic acid, a quinone epoxide inhibitor of Bruton's tyrosine kinase. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2227-32. doi: 10.1073/pnas.96.5.2227. [PubMed:10051623 ]
- Boruta T, Bizukojc M: Culture-based and sequence-based insights into biosynthesis of secondary metabolites by Aspergillus terreus ATCC 20542. J Biotechnol. 2014 Apr 10;175:53-62. doi: 10.1016/j.jbiotec.2014.01.038. Epub 2014 Feb 15. [PubMed:24534845 ]
- Guo CJ, Sun WW, Bruno KS, Wang CC: Molecular genetic characterization of terreic acid pathway in Aspergillus terreus. Org Lett. 2014 Oct 17;16(20):5250-3. doi: 10.1021/ol502242a. Epub 2014 Sep 29. [PubMed:25265334 ]
- Olesen SH, Ingles DJ, Yang Y, Schonbrunn E: Differential antibacterial properties of the MurA inhibitors terreic acid and fosfomycin. J Basic Microbiol. 2014 Apr;54(4):322-6. doi: 10.1002/jobm.201200617. Epub 2013 May 20. [PubMed:23686727 ]
|
